Cardiorenal and Metabolic Diseases Research Center

项目摘要/摘要 心血管疾病、肾脏疾病和代谢性疾病在美国非常普遍，尤其是在密西西比州。我们 在密西西比大学建立了心脏肾脏和代谢性疾病研究中心 医学中心将一个由基础、临床和人口科学家组成的多学科小组聚集在一起，致力于 心血管疾病、肾脏疾病和代谢疾病。了解这些慢性病之间的复杂关系 需要基础、临床和人口科学家的共同努力，使用创新的多学科 综合的研究方法和最先进的技术，促进发现。分子 心血管疾病、肾脏疾病和代谢性疾病相互依赖的基础已经通过基因组学进行了经典的研究， 转录学，以及评估DNA序列和RNA和蛋白质水平的蛋白质组学平台 表情。虽然这些研究产生的数据是信息性的，但对复杂生物系统的调节 还需要了解蛋白质的活性。蛋白激酶是控制蛋白质活性网络的核心 并连接几个调控元件，包括转录因子、伴侣和结构蛋白。这个 Kinome指的是一整套蛋白激酶的广泛活性。最近有几种方法 是根据蛋白激酶的巨大活性来研究动态组的。其中一项新技术 是PamChip，它使用数百个~13个残基长的报告多肽，这些多肽被磷酸化 从细胞培养、组织或临床活检中提取的样本。磷酸化程度是以 实时使用荧光抗体。该技术包含在PamGene的PAMStation12中。 本设备补充申请是为CMDRC购买PAMStation 12的资金。此次收购 这一最先进的系统将使父母眼镜蛇的调查人员能够确定Kinome的角色 在心血管疾病、肾脏疾病和代谢疾病方面。这一新设备还将增加 实验方法和加强来自CMDRC研究人员和其他人员的未来NIH拨款 UMMC的调查人员，包括由NIGMS资助的其他IDEA中心支持的调查人员。CMDRC已经被 这是密西西比州心脏肾脏和代谢性疾病研究显著扩大的主要驱动力。这么大 设备赠款将扩大并确保我们努力发展一个高生产率的CMDRC的可持续性 致力于通过研究、发现和创新来改善生活。

PROJECT SUMMARY/ABSTRACT Cardiovascular (CV), renal and metabolic diseases are highly prevalent in the U.S., especially in Mississippi. We established the Cardiorenal and Metabolic Diseases Research Center (CMDRC) at the University of Mississippi Medical Center to bring together a multidisciplinary group of basic, clinical and population scientists working on CV, renal, and metabolic diseases. Understanding the complex relationship between these chronic diseases requires the combined efforts of basic, clinical and population scientists using innovative multidisciplinary integrative approaches to research and state-of-the-art technologies that facilitate discovery. The molecular bases for interdependence of CV, renal, and metabolic diseases have classically been studied via genomics, transcriptomics, and proteomics platforms that assess DNA sequences and levels of RNA and protein expression. While data generated from these studies are informative, regulation of complex biological systems also requires understanding protein activity. Protein kinases are central to governing protein activity networks and link several regulatory elements, including transcription factors, chaperones, and structural proteins. The kinome refers to the broad-based activity of the complete set of protein kinases. Several methods have recently been developed to study the kinome in light of the vast activity of protein kinases. One of these new technologies is the PamChip which uses hundreds of ~13 residue-long reporter peptides which are phosphorylated with samples derived from cell cultures, tissues, or clinical biopsies. The degree of phosphorylation is measured in real-time using fluorescent antibodies. This technology is encompassed in the PAMStation 12 from Pamgene. This equipment supplement application is for funds to purchase a PAMStation 12 for the CMDRC. The acquisition of this state-of-the-art system will allow investigators of the parent COBRE to determine the role of the kinome in the areas of CV, renal, and metabolic diseases. This new equipment will also increase the sophistication of experimental approaches and strengthen future NIH grants from CMDRC investigators as well as other investigators at UMMC, including those supported by other NIGMS funded IDeA centers. The CMDRC has been a major driver for marked expansion of cardiorenal and metabolic diseases research in Mississippi. This large equipment grant will amplify and ensure sustainability of our efforts to develop a highly productive CMDRC dedicated to improving lives through research, discovery, and innovation.