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Research Supplements to Promote Diversity

促进多样性的研究补充

基本信息

批准号：
10889729
负责人：
Mathew Thoppil Mathew
金额：
$ 8.47万
依托单位：
ILLINOIS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10889729
关键词：
Actinobacillus actinomycetemcomitans Address Award Biological Markers Categories Cells Clinical Collection Complex Data Detection Development Devices Dimensions Enzymes Event Forsythia Fusobacterium nucleatum Future Gingiva Glass Goals Health Health Sciences Individual Industry Inflammation Ions Knowledge Label Lasers Machine Learning Measurement Measures Mediator Medical Metals Methods MicroRNAs Microfluidic Microchips Microfluidics Modality Molecular Monitor Output Parents Particle Size Patients Periodontal Diseases Periodontal Pocket Periodontitis Phenotype Play Porphyromonas gingivalis Preparation Prevotella intermedia Protocols documentation Raman Spectrum Analysis Reaction Research Research Project Grants Research Support Resolution Role Saliva Salivary Sampling Signal Transduction Signaling Protein Slide Spectrum Analysis Streptococcus mutans Structure Surface Testing Tissues Tooth structure Training Translational Research Treponema denticola bone career clinical application comorbidity density design detection method doctoral student experience high throughput screening imaging approach improved insight interdisciplinary collaboration lithography machine learning method machine learning model medical specialties microbial microorganism mobile sensor multiplex detection nano nanoparticle novel parent grant pathogen periodontopathogen personalized medicine point of care potential biomarker pre-doctoral prototype saliva sample sensor soft tissue tool

项目摘要

On-chip multiplex detection of periodontal pathogens by Raman imaging at single cell level Project Summary: Periodontitis is initiated by complex bacterial interaction in tissues surrounding the teeth. Periodontal pathogens accumulate in periodontal pockets and induce periodontal destruction in a cooperative manner, leading to gingival inflammation, soft tissue destruction, and bone destruction. These pathogens can serve as biomarkers to inform periodontal status. Surface- enhanced Raman spectroscopy (SERS) has emerged as a powerful label-free detection tool to discriminate bacterial phenotypes due to the Raman signatures of their unique molecular composition. To facilitate SERS utility in clinical applications, it is imperative to develop a platform that warrants simple and universal sample preparation method with minimal technical demand. We address this critical need in this study by pre-loading Ag nanoparticles (Ag-NPs) on all bacterial cells in a sample in a one-pot reaction. The reaction is quick and no species-specific label is needed. Following the parent grant's microbial compartmentalization concept, the pathogens will be disseminated to microchambers that are interconnected by parallel microchannels. By design, each microchamber accommodates one cell. Ag-NP enables the collection of well-resolved Raman spectrum at a single-cell level (Aim 1). Machine learning in combination with the snap-shot hyperspectral imaging approach will enable confident identification and enumeration of pathogen species with spatial resolution to facilitate on-chip multiplex detection (Aim 2). This is a versatile detection method that can be integrated on a portable sensor device as proposed in the parent grant. Integrating with other components of the parent grant, this research contributes to the development of a prototype salivary sensor that will offer detailed medical data from frequent and progressive monitoring, and it will be an effective tool for future personalized medicine and is expected to dramatically improve the patient's overall health. In addition to technical training, the predoctoral student will gain experience through engaging in interdisciplinary collaborations, providing him with insights into the intricacies of workflow within an interdisciplinary project and the pivotal role that each specialty subject would play in supporting research in diverse fields, preparing him to become capable of tackling multifaceted challenges in his future career.

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