The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

项目摘要 物理活动联盟的分子传感器（MoTrPAC）旨在发现 和表征的范围内的分子换能器（“分子地图”），其基础 身体活动（PA）对人体的影响。我们的运动和体育活动协作 团队（ExPACT）-伯明翰亚拉巴马大学运动中心联盟 医学（UAB），奥兰多代谢和糖尿病转化研究所， 佛罗里达（TRI-MD）和鲍尔州立大学人类行为实验室（BSU）-已经 在进行高度控制的有氧（AT）和阻力（RT）运动方面拥有丰富的经验 在健康和患病人群中进行临床试验。ExPACT的总体目标 为成功的MoTrPAC规划、执行和创新做出实质性贡献。 第一阶段已经完成，包括：目标1）有效地与 MoTrPAC领导和NIH领导帮助指导和最终确定结构和标准 操作程序（SOP）。在项目的剩余年份，ExPACT将实施 并完成第二阶段和第三阶段，如下：第二阶段（招募和测试年） 1.我们将招募420名久坐但健康的18岁以上男性和女性， AT（n=140）、RT（n = 140）或无运动对照（CON，n=50）的12周随机试验。 ExPACT地理将丰富MoTrPAC种族，民族，城市，郊区和农村的多样性。 该设计结合了全面的表型分析，以评估健身和临床结果， 和人体组织表型分析，将分子传感器与关键生物反应联系起来， 锻炼身体目标二。训练前和训练后，我们将获得战略性定时生物标本， 急性运动后。为了更好地了解生物极端，我们将进行 在训练有素的运动员中进行的相同急性反应研究（AT（n=25）或RT（n=25））。串行 CON中的生物标本采集将允许估计生物学、季节性、昼夜节律和 方法的可变性。目标3.为了提高MoTrPAC的整体科学影响力，我们将 继续与财团合作进行辅助研究， 增加受试者和组织表型和分子图谱的解释。III期 （结束语）目标1。我们将完成所有ExPACT数据输入、质量控制和中央存款 与MoTrPAC协调中心合作，我们将积极 有助于完成生物标本分析、撰写手稿和传播 结果我们很荣幸能与MoTrPAC合作，为未来的知识贡献力量。 身体活动可以改善或保持健康的分子机制。

Project Summary The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the ‘molecular map’) that underlie the effects of physical activity (PA) in humans. Our Exercise and Physical Activity Collaborative Team (ExPACT) –an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) –has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. The overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase 1 has already been completed and consisted of: Aim 1) Effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). In the remaining years of the project, the ExPACT will implement and complete Phase II and Phase III, as follows: Phase II (Recruitment and Testing Years) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=140), RT (n=140), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre-and posttraining, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=25) or RT (n=25)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will continue to work with the consortium to conduct ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.