The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

项目摘要 体力活动联盟的分子传感器(MoTrPAC)旨在发现 并描述了分子换能器的范围(分子图谱) 身体活动(PA)对人类的影响。我们的运动和身体活动协同 团队(ExPACT)-阿拉巴马大学伯明翰中心锻炼联盟 医学(UAB)，奥兰多新陈代谢和糖尿病转化研究所， 佛罗里达(TRI-MD)和鲍尔州立大学人类行为实验室(BSU)-有 具有进行高度可控的有氧(AT)和阻力(RT)运动的丰富经验 培训健康和患病人群的临床试验。ExPACT的总体目标 是为MoTrPAC的成功规划、执行和创新做出实质性贡献。 第一阶段已经完成，包括：目标1)有效协作 MoTrPAC领导人和NIH领导人帮助指导和最终确定结构和标准 操作程序(SOP)。在该项目的剩余几年中，ExPACT将实施 并完成第二阶段和第三阶段，具体如下：第二阶段(征聘和考试年)目标 1.我们将招募420名久坐但身体健康的18+岁男性和女性参加严格控制的 12周，随机试验，AT(n=140)，RT(n=140)，或不运动对照(Con，n=50)。 ExPACT地理将丰富MoTrPAC的种族、民族、城市、郊区和农村多样性。 该设计结合了全面的表型分析来评估健康状况和临床结果， 以及人类组织表型分析，将分子换能器与关键的生物反应联系起来 锻炼身体。目标2.在培训前和培训后，我们将在培训前和培训后获得具有战略意义的定时生物武器 在剧烈运动之后。为了更好地了解生物极端情况，我们将进行 在训练有素的运动员(AT(n=25)或RT(n=25))中进行相同的急性反应研究。连载 在CON中收集生物样品将能够估计生物、季节、昼夜和 方法论的可变性。目标3.为了加强MoTrPAC的整体科学影响，我们将 继续与财团合作进行辅助研究，这将显著 增强受试者和组织的表型和分子图谱的解释。第三阶段 目标1.我们将完成所有ExPACT数据录入、质量控制和集中存放 与MoTrPAC协调中心合作的程序，我们将积极 协助完成生物谱学分析、手稿撰写和传播 结果。我们将非常荣幸地与MoTrPAC合作，贡献未来关于 体力活动可以改善或保持健康的分子机制。

Project Summary The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the ‘molecular map’) that underlie the effects of physical activity (PA) in humans. Our Exercise and Physical Activity Collaborative Team (ExPACT) –an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) –has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. The overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase 1 has already been completed and consisted of: Aim 1) Effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). In the remaining years of the project, the ExPACT will implement and complete Phase II and Phase III, as follows: Phase II (Recruitment and Testing Years) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=140), RT (n=140), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre-and posttraining, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=25) or RT (n=25)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will continue to work with the consortium to conduct ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.