The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

锻炼和身体活动协作团队 (ExPACT)：拟建 MoTrPAC 临床中心

• 批准号: 10893749
• 负责人: Thomas W Buford
• 金额: $ 9.78万
• 依托单位: BALL STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10893749
• 关键词: Acute; Aerobic; Alabama; Ancillary Study; Biological; Clinical; Clinical Trials; Collaborations; Collection; Coupled; Data; Deposition; Diabetes Mellitus; Disease; Enrollment; Ethnic Origin; Exercise; Florida; Future; Geography; Goals; Health; Health Benefit; Human; Information Dissemination; Knowledge; Laboratories; Leadership; Link; Manuscripts; Maps; Medicine; Metabolism; Methodology; Molecular; Outcome; Performance; Phase; Phenotype; Physical activity; Population; Procedures; Public Health; Quality Control; Race; Research Institute; Resistance; Rural; Seasons; Structure; Testing; Tissues; Training; Transducers; Translational Research; United States National Institutes of Health; Universities; Woman; Work; Writing; circadian; clinical center; clinical training; design; exercise training; experience; fitness; human tissue; improved; innovation; men; preservation; randomized trial; recruit; resistance exercise; response; sedentary; suburb

Project Summary The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the ‘molecular map’) that underlie the effects of physical activity (PA) in humans. Our Exercise and Physical Activity Collaborative Team (ExPACT) –an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) –has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. The overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase 1 has already been completed and consisted of: Aim 1) Effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). In the remaining years of the project, the ExPACT will implement and complete Phase II and Phase III, as follows: Phase II (Recruitment and Testing Years) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=140), RT (n=140), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre-and posttraining, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=25) or RT (n=25)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will continue to work with the consortium to conduct ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.

项目总结

项目成果

Human adipose and skeletal muscle tissue DNA, RNA, and protein content.

人体脂肪和骨骼肌组织 DNA、RNA 和蛋白质含量。

• DOI: 10.1152/japplphysiol.00343.2021
• 发表时间: 2021
• 期刊: Journal of applied physiology (Bethesda, Md. : 1985)
• 作者: Stroh,AndrewM;Lynch,ColleenE;Lester,BridgetE;Minchev,Kiril;Chambers,TobyL;Montenegro,CristhianF;ChavezMartinez,Clarisa;Fountain,WilliamA;Trappe,ToddA;Trappe,ScottW
• 通讯作者: Trappe,ScottW

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling.

• DOI: 10.1016/j.xpro.2023.102054
• 发表时间: 2023-03-17
• 期刊: STAR PROTOCOLS
• 作者: Whytock, Katie L.;Divoux, Adeline;Sun, Yifei;Hopf, Meghan;Yeo, Reichelle X.;Pino, Maria F.;Yu, Gongxin;Smith, Steven R.;Walsh, Martin J.;Sparks, Lauren M.
• 通讯作者: Sparks, Lauren M.