CYTOGENETIC EFFECTS OF ALCOHOL ON A HUMAN CHROMOSOME

申请人摘要：格兰布林州立大学，格兰布林， 路易斯安那州，历史上的黑人学院和大学之一。 的 GSU的科学研究传统处于早期发展阶段。 因此，该应用程序由两个部分组成：（1）研究 项目：酒精对人类染色体的细胞遗传学影响，以及（11）a GSU酒精和生物行为研究的研究开发计划。 该研究项目的目的是研究，通过使用荧光在 用染色体特异性DNA探针进行原位杂交， 乙醇对人类4号染色体的细胞遗传学影响。 在这项研究中， 一种特殊的中国仓鼠-人杂交细胞系，含有21-26个中国人 将使用仓鼠染色体和人4号染色体的单拷贝。 人类4号染色体具有特殊的意义，它携带一簇ADH 可能调节乙醇作用的基因。 染色体畸变 表明细胞暴露于DNA损伤的细胞遗传学终点 是目前最可靠的诱变和 致癌化合物 本研究中使用FISH技术是一种 新颖的方法。 该研究项目将于年在GSU进行。 与放射生物学和环境卫生实验室合作， 加州大学旧金山分校弗朗西斯科。 研究的目标 GSU酒精和生物行为科学的发展方向是：（i） 加强酒精和生物行为研究;（二）发展、扩大和 提高教师在这些领域开展研究的能力; ㈢ 扩大和加强机构研究基础设施;（iv） 鼓励少数民族学生和妇女在这些领域进行研究; 以及（v）增加少数民族和女学生的入学人数 这些领域的研究工作。

APPLICANT'S ABSTRACT: Grambling State University (GSU), Grambling, Louisiana, one of the Historically Black Colleges and Universities. The tradition of scientific research at GSU is in its early development. Therefore, this application consists of two components: (1) a RESEARCH PROJECT: Cytogenetic Effects of Alcohol on a Human Chromosome, and (11) a RESEARCH DEVELOPMENT PLAN for alcohol and bio-behavioral research at GSU. The purpose of the research project is to study, by using fluorescence in situ hybridization (FISH) with chromosome-specific DNA probe, the cytogenetic effects of ethanol on hum in chromosome 4. For this study a special Chinese hamster - human hybrid cell line that contains 21-26 Chinese hamster chromosomes and a single copy of human chromosome 4 will be used. Human chromosome 4 has special significance; it carries a cluster of ADH genes which may modulate the effects of ethanol. Chromosome aberrations are cytogenetic end points that indicate cellular exposure to DNA damaging agents and are currently the most reliable indicators of mutagenic and carcinogenic compounds. The use of the FISH technique in this study is a novel approach. The research project will be carried out at GSU in collaboration with the Laboratory of Radiobiology and Environmental Health, University of California, San Francisco. The objectives of RESEARCH DEVELOPMENT PLAN for alcohol and bio-behavioral sciences at GSU are: (i) to strengthen alcohol and bio-behavioral research; (ii) to develop, expand and increase the capacity of faculty to conduct research in those areas; (iii) to augment and strengthen the institutional research infrastructure; (iv) to encourage minority students and women to conduct research in those areas; and (v) to increase the number of minority and women students who will enter research careers in those areas.