CYTOGENETIC EFFECTS OF ALCOHOL ON A HUMAN CHROMOSOME

酒精对人类染色体的细胞遗传学影响

• 批准号: 2457497
• 负责人: KRISHAN M SAXENA
• 金额: $ 9.6万
• 依托单位: GRAMBLING STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2457497
• 关键词: DNA damage; X ray; alcohol dehydrogenase; alcoholism /alcohol abuse; aneuploidy; animal tissue; chromosome aberrations; chromosome deletion; chromosome translocation; cytogenetics; density gradient ultracentrifugation; drug interactions; enzyme activity; ethanol; fluorescent in situ hybridization; genetic library; human genetic material tag; human tissue; hybrid cells; mutagens; nucleic acid probes; statistics /biometry

APPLICANT'S ABSTRACT: Grambling State University (GSU), Grambling, Louisiana, one of the Historically Black Colleges and Universities. The tradition of scientific research at GSU is in its early development. Therefore, this application consists of two components: (1) a RESEARCH PROJECT: Cytogenetic Effects of Alcohol on a Human Chromosome, and (11) a RESEARCH DEVELOPMENT PLAN for alcohol and bio-behavioral research at GSU. The purpose of the research project is to study, by using fluorescence in situ hybridization (FISH) with chromosome-specific DNA probe, the cytogenetic effects of ethanol on hum in chromosome 4. For this study a special Chinese hamster - human hybrid cell line that contains 21-26 Chinese hamster chromosomes and a single copy of human chromosome 4 will be used. Human chromosome 4 has special significance; it carries a cluster of ADH genes which may modulate the effects of ethanol. Chromosome aberrations are cytogenetic end points that indicate cellular exposure to DNA damaging agents and are currently the most reliable indicators of mutagenic and carcinogenic compounds. The use of the FISH technique in this study is a novel approach. The research project will be carried out at GSU in collaboration with the Laboratory of Radiobiology and Environmental Health, University of California, San Francisco. The objectives of RESEARCH DEVELOPMENT PLAN for alcohol and bio-behavioral sciences at GSU are: (i) to strengthen alcohol and bio-behavioral research; (ii) to develop, expand and increase the capacity of faculty to conduct research in those areas; (iii) to augment and strengthen the institutional research infrastructure; (iv) to encourage minority students and women to conduct research in those areas; and (v) to increase the number of minority and women students who will enter research careers in those areas.

申请者摘要：格拉姆林州立大学(GSU)， 路易斯安那州，历史上的黑人学院和大学之一。这个 格拉斯哥州立大学的科学研究传统处于早期发展阶段。 因此，本申请由两部分组成：(1)研究 项目：酒精对人类染色体的细胞遗传学效应，以及(11)a 格拉斯哥州立大学酒精和生物行为研究的研究发展计划。 这项研究项目的目的是研究通过使用荧光在 与染色体特异DNA探针的原位杂交(FISH) 乙醇对4号染色体HUM的细胞遗传学效应 特殊的中国仓鼠-人杂交细胞系，含有21-26个中国人 将使用仓鼠染色体和人类4号染色体的单一副本。 人类4号染色体具有特殊的意义；它携带一簇ADH 可能调节乙醇效应的基因。染色体的异常是 表明细胞暴露于DNA损伤的细胞遗传学终点 目前是最可靠的致突变和 致癌化合物。在这项研究中使用FISH技术是一种 新的方法。该研究项目将于#年在格陵兰州立大学进行。 与放射生物学和环境健康实验室合作， 加州大学旧金山分校。研究的目标 格拉斯哥州立大学酒精和生物行为科学的发展计划是：(I) 加强酒精和生物行为研究；(2)开发、扩大和 提高教员在这些领域进行研究的能力； 扩充和加强院校研究基础设施；(4) 鼓励少数族裔学生和妇女在这些领域开展研究； 和(5)增加少数族裔和女学生的入学人数 在这些领域的研究事业。