Development and Regeneration of the Endocrine Pancreas

概述了胰岛发育和再生领域的一项研究计划，该计划结合了基因/蛋白质发现计划、转录调控和细胞信号传导研究，并解决了通过离体扩增和分化将β细胞移植到人类受试者中的实际目标：项目1（Hutton/Duncan）：发育中内分泌胰腺的蛋白质组学和基因组分析。 PROJECT 2 (Sussell/Lee): Analysis of the Functions of NeuroD and Nkx2.2 in the Maintenance and Regeneration of the Adult Islet. PROJECT 3 (Levine/Stuart): Human beta-Cell Lines. PROJECT 4 (Jensen): Induction of endocrine neogenesis of endocrine neogenesis from adult pancreas.支持两个核心，其中一个是管理信息学，第二个为 β 细胞分离和功能分析领域的所有项目提供服务。该项目直接涉及 Beta 细胞生物学联盟中概述的 6 个科学研究目标领域中的 2 个（Beta 细胞生物学联盟中概述的科学研究的 β 细胞发育/细胞培养（人类胰腺 β 细胞的 β 细胞发育/细胞培养模型））。此外，他们将使用与 RFA 中两个进一步目标相关的方法生成数据（干/祖细胞的识别/移植胰岛的评估）。项目中使用的蛋白质组和基因组方法1 将产生大量数据，这些数据将放置在由核心 A 的信息学部分支持的网站上，可供联盟成员和广大糖尿病研究界进行挖掘。主要研究人员和共同研究人员包括来自各个学科（生物化学/遗传学/分子生物学/细胞生物学/发育生物学）的高级和初级研究人员，该计划将主要位于 1 型糖尿病的细胞疗法。丹佛芭芭拉·戴维斯儿童糖尿病中心将在免疫学、人类遗传学和移植生物学领域与临床医生、临床研究人员和基础科学家进行进一步的互动。BDC 是 NIH 糖尿病和内分泌研究中心，将通过动物资源、细胞计数、分子资源和临床核心为该计划提供支持。

A Program of research is outlined in the area of pancreatic islet development and regeneration that incorporates a gene/protein discovery program, studies in transcription regulation and cell signaling and addresses the practical goal of engineering beta-cell for transplantation into human subjects through expansion and differentiation ex vivo: PROJECT 1 (Hutton/Duncan): Proteomic and Genomic Analysis of the Developing Endocrine Pancreas. PROJECT 2 (Sussell/Lee): Analysis of the Functions of NeuroD and Nkx2.2 in the Maintenance and Regeneration of the Adult Islet. PROJECT 3 (Levine/Stuart): Human beta-Cell Lines. PROJECT 4 (Jensen): Induction of endocrine neogenesis of endocrine neogenesis from adult pancreas. Two Cores are supported one in are of Administration Informatics), a second providing services to all the projects in the area beta-cell isolation and function analysis. The project directly addresses 2 of the 6 target areas of scientific investigation outlined in the Beta Cell Biology Consortium (beta cell development/cell culture of scientific investigation outlined in the Beta Cell Biology Consortium (beta cell development/cell culture model of the human pancreatic-beta cell). In addition they will generate data using methodologies of relevance to two further aims in the RFA (identification of stem/progenitor cells/evaluation of pancreatic islets for transplantation). The proteomic and genomic approach used in Project 1 will produce a wealth of data that will be placed on a web site supported by the informatics component of Core A that will be accessible for mining by Consortium members and the diabetes research community at large. The principal investigators and co-investigators include both senior and more junior investigators who come from a wide range of disciplines (biochemistry/genetics/molecular biology/cell biology/developmental biology) linked by the common goal of producing cell-based therapy for type 1 diabetes. The Program will be located principally at the Barbara Davis Center for Childhood Diabetes in Denver which will provide further interactions with clinicians, clinical researchers and basic scientists in the area of immunology, human genetics and transplant biology. The BDC is a NIH Diabetes and Endocrine Research Center that will provide support for the Program through Animal Resources, Cytometry, Molecular Resources and a Clinical Core.