Development and Regeneration of the Endocrine Pancreas

概述了胰岛发育和再生领域的研究计划，该计划包括基因/蛋白质发现计划，转录调节和细胞信号传导研究，并解决了通过体外扩增和分化将工程β细胞移植到人类受试者中的实际目标：项目1（赫顿/邓肯）：发育中的内分泌胰腺的蛋白质组学和基因组学分析。项目2（Sussell/Lee）：NeuroD和Nkx2.2在成人胰岛维持和再生中的功能分析。项目3（Levine/Stuart）：人β细胞系。项目4（詹森）：诱导成年胰腺内分泌新生。两个核心得到支持，一个是行政信息学，另一个为β细胞分离和功能分析领域的所有项目提供服务。该项目直接涉及β细胞生物学联盟概述的6个科学调查目标领域中的2个（β细胞发育/细胞培养），以及β细胞生物学联盟概述的科学调查目标领域（人类胰腺β细胞的β细胞发育/细胞培养模型）。此外，他们将使用与RFA中的另外两个目标相关的方法生成数据（干细胞/祖细胞的鉴定/胰岛移植的评价）。项目1中使用的蛋白质组学和基因组学方法将产生大量数据，这些数据将被放置在由核心A的信息学组成部分支持的网站上，该网站将被联盟成员和糖尿病研究界广泛访问以进行挖掘。主要研究者和共同研究者包括来自广泛学科（生物化学/遗传学/分子生物学/细胞生物学/发育生物学）的高级和初级研究者，这些学科由生产1型糖尿病细胞疗法的共同目标联系在一起。该计划将主要位于丹佛的芭芭拉戴维斯儿童糖尿病中心，该中心将与免疫学，人类遗传学和移植生物学领域的临床医生，临床研究人员和基础科学家进行进一步的互动。BDC是NIH糖尿病和内分泌研究中心，将通过动物资源，细胞计数，分子资源和临床核心为该计划提供支持。

A Program of research is outlined in the area of pancreatic islet development and regeneration that incorporates a gene/protein discovery program, studies in transcription regulation and cell signaling and addresses the practical goal of engineering beta-cell for transplantation into human subjects through expansion and differentiation ex vivo: PROJECT 1 (Hutton/Duncan): Proteomic and Genomic Analysis of the Developing Endocrine Pancreas. PROJECT 2 (Sussell/Lee): Analysis of the Functions of NeuroD and Nkx2.2 in the Maintenance and Regeneration of the Adult Islet. PROJECT 3 (Levine/Stuart): Human beta-Cell Lines. PROJECT 4 (Jensen): Induction of endocrine neogenesis of endocrine neogenesis from adult pancreas. Two Cores are supported one in are of Administration Informatics), a second providing services to all the projects in the area beta-cell isolation and function analysis. The project directly addresses 2 of the 6 target areas of scientific investigation outlined in the Beta Cell Biology Consortium (beta cell development/cell culture of scientific investigation outlined in the Beta Cell Biology Consortium (beta cell development/cell culture model of the human pancreatic-beta cell). In addition they will generate data using methodologies of relevance to two further aims in the RFA (identification of stem/progenitor cells/evaluation of pancreatic islets for transplantation). The proteomic and genomic approach used in Project 1 will produce a wealth of data that will be placed on a web site supported by the informatics component of Core A that will be accessible for mining by Consortium members and the diabetes research community at large. The principal investigators and co-investigators include both senior and more junior investigators who come from a wide range of disciplines (biochemistry/genetics/molecular biology/cell biology/developmental biology) linked by the common goal of producing cell-based therapy for type 1 diabetes. The Program will be located principally at the Barbara Davis Center for Childhood Diabetes in Denver which will provide further interactions with clinicians, clinical researchers and basic scientists in the area of immunology, human genetics and transplant biology. The BDC is a NIH Diabetes and Endocrine Research Center that will provide support for the Program through Animal Resources, Cytometry, Molecular Resources and a Clinical Core.