Synaptic connectivity in accessory olfactory bulb

附件嗅球中的突触连接

• 批准号: 6650550
• 负责人: Ricardo C Araneda
• 金额: $ 8.18万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6650550
• 关键词: biological signal transduction; cell cell interaction; chemoreceptors; confocal scanning microscopy; electrophysiology; fluorescent dye /probe; genetically modified animals; green fluorescent proteins; morphology; neural information processing; neuroanatomy; neurons; neuropharmacology; neurophysiology; olfactions; olfactory lobe; sectioning; synapses; vomeronasal systems

DESCRIPTION (provided by applicant): The accessory olfactory bulb (AOB) is the first relay of chemosensory information in the vomeronasal system, yet very few studies have addressed cell physiology and synaptic connectivity of cells in the AOB. Principal neurons in the AOB, unlike cells in the main olfactory bulb (MOB), send primary dendrites to more than one glomerulus, and peripheral sensory neurons expressing a single vomeronasal receptor (VR) in the vomeronasal organ (VNO) project to multiple glomeruli. There is also a segregation of projections from sensory neurons in the VNO into the anterior and posterior aspects of the AOB, and this segregation may also result in differential patterns of activity in these regions. The overall aim of this application is to study the physiological properties of principal neurons in the AOB. We will relate physiological characteristics to anatomical parameters of the cells, particularly glomerular connectivity, and their distribution in the anterior-posterior axis of the AOB. In Specific Aim 1, we will determine if mitral cells are connected only to glomeruli that receive inputs from the same receptor, or if the same mitral cell maintains connection with a mixed population of receptors. We will use two lines of transgenic mice, the V1r and V2r-GFP mice. Recording from mitral cells connected to GFP-labeled glomeruli and including a fluorescent dye in the intracellular recording solution will allow identification of the cell's glomerular connection(s). We will compare the number of GFP-labeled glomeruli with those labeled by the intracellular dye. In Specific Aim 2, we will study the pharmacological and physiological properties of dendrodendritic inhibition in mitral cells. Because VNO neurons expressing the V1r and V2r project to the anterior and posterior AOB respectively, the use of these transgenic mice will allow us to compare these subdivisions of the AOB. It is anticipated that these studies will have an impact on our understanding of the sensory coding of pheromonal information and other olfactory information by the accessory olfactory system.

描述（由申请人提供）：辅助嗅球（AOB）是犁鼻系统中化学感应信息的第一个中继站，但很少有研究涉及 AOB 中细胞的生理学和突触连接。与主嗅球 (MOB) 中的细胞不同，AOB 中的主要神经元将初级树突发送到多个肾小球，而犁鼻器官 (VNO) 中表达单个犁鼻受体 (VR) 的外周感觉神经元投射到多个肾小球。 VNO 中的感觉神经元到 AOB 的前部和后部的投射也存在分离，这种分离也可能导致这些区域的活动模式不同。该应用的总体目标是研究 AOB 中主要神经元的生理特性。我们将把生理特征与细胞的解剖参数联系起来，特别是肾小球的连接性，以及它们在 AOB 前后轴上的分布。在具体目标 1 中，我们将确定二尖瓣细胞是否仅与从同一受体接收输入的肾小球连接，或者同一二尖瓣细胞是否与混合的受体群保持连接。我们将使用两个转基因小鼠品系，V1r 和 V2r-GFP 小鼠。从连接到 GFP 标记的肾小球的二尖瓣细胞进行记录，并在细胞内记录溶液中加入荧光染料，将允许识别细胞的肾小球连接。我们将比较 GFP 标记的肾小球数量与细胞内染料标记的肾小球数量。在具体目标 2 中，我们将研究二尖瓣细胞中树突状细胞抑制的药理学和生理学特性。由于表达 V1r 和 V2r 的 VNO 神经元分别投射到前 AOB 和后 AOB，因此使用这些转基因小鼠将使我们能够比较 AOB 的这些细分。预计这些研究将影响我们对辅助嗅觉系统对信息素信息和其他嗅觉信息的感觉编码的理解。