OLFACTORY AXON REGULATE OLFACTORY BULB CYTOGENESIS

描述（由申请人提供）：神经发生和 发育差异化对于形成精准的 神经回路最近的研究提供了证据， 与靶组织的相互作用可以调节神经发生。的具体目标 这个应用程序被设计来研究嗅觉轴突 与发育中的端脑相互作用，以调节 嗅球原基，从嗅球的喙端发育而来。 端脑我们将使用体外实验方法，并将这些方法联合收割机 细胞周期动力学分析。主要的实验目标是：1） 研究早期嗅觉轴突在调节细胞周期动力学中的作用 在嗅球原基中; 2）研究早期嗅觉细胞是否 轴突在决定嗅球中的神经元 原基成为僧帽细胞; 3）决定先驱嗅觉细胞是否 轴突在调节嗅觉神经元的形态发生方面是独特的 灯泡这项工作的长期目标是阐明外在因素的作用 信号调节机制的神经生产中枢神经系统，重点是 嗅球我们希望通过这项研究获得的证据将 提供了关于中枢神经系统细胞的细胞周期是如何调节的见解。的 确定细胞发生过程中的关键调控步骤将有助于 进一步分析参与这一过程的分子。

DESCRIPTION (provided by applicant): The coordination of neurogenesis and differentiation in development is important for the formation of precise neuronal circuitry. Recent studies have provided evidence that axonal interaction with target tissue can regulate neurogenesis. The specific aims of this application are designed to examine the mechanisms by which olfactory axons interact with the developing telencephalon to regulate neurogenesis in the olfactory bulb primordium that develops from the rostral end of the telencephalon. We will use in vitro experimental approaches and combine these with cell cycle kinetics analysis. The major experimental goals are to: 1) examine the role of the early olfactory axons in regulating cell cycle kinetics in the olfactory bulb primordium; 2) investigate whether the early olfactory axons play a role in determining whether neurons in the olfactory bulb primordium become mitral cells; and 3) determine whether the pioneer olfactory axons are unique in their ability to regulate the morphogenesis of the olfactory bulb. The long-term goals of this work are to elucidate the roles of extrinsic signals in regulating mechanisms of neural production in CNS, with a focus on the olfactory bulb. We expect that evidence obtained through this study will provide insights into how the cell cycles of CNS cells are regulated. The determination of critical regulatory steps during cytogenesis will facilitate further analysis of the molecular players involved in this process.