Laser Device for Ion Channel Activation

用于离子通道激活的激光装置

• 批准号: 6693127
• 负责人: Mikhail I. Nemenov
• 金额: $ 10万
• 依托单位: LASMED, LLC
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693127
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; cell membrane; heat stimulus; lasers; light intensity; membrane channels; single cell analysis

DESCRIPTION (provided by applicant): The goal of this Phase 1 SBIR is to design and test a prototype of the Channel Laser Stimulator capable of stimulating single cells or part of the cell. This tool is needed to study dose dependency of heat/capsaicin-sensitive cells/neurons and "calcium" channels. Understanding how these "calcium" channels work and principles of their kinetics is important in neuroscience in general and especially in therapeutics and drug development. Further, the development of this instrument will enable the manipulation of cell activity in culture or in tissues with light, a method that could lead to important new applications for regulating cellular or physiological processes in vivo. This device may also lead to novel methods of drug screening and development as it could provide a rapid, well calibrated, and reproducible method of delivering a thermal pulse to cells or purified ion channel protein in single or multi-well format. We will design and test a Channel Laser Stimulator that (A) will deliver a consistent light intensity directly to the target-membrane and channels of the cell; (B) will enable the operator to precisely control the heat stimulus and thus activate some (predictable) amount of channel activity; and (C) will enable the operator to calibrate heat stimulus by power and pulse duration of the laser pulses. We will design this prototype of the Channel Laser Stimulator with parameters optimized for single cell level research, based on (1) the design of an infrared laser diode pain stimulator, developed by LASMED, and (2) the results of preliminary testing. We will consult with a leading scientist in the field of molecular investigations of thermo sensitive ion channels in the mammalian nervous system. All necessary tests to verify performance of the Channel Laser Stimulator will be carried out in the laboratory of this scientist by his personnel.

描述（由申请人提供）：本阶段1 SBIR的目标是设计和测试能够刺激单细胞或部分细胞的通道激光刺激器原型。需要这种工具来研究热/辣椒素敏感细胞/神经元和“钙”通道的剂量依赖性。了解这些“钙”通道如何工作及其动力学原理在一般神经科学中非常重要，特别是在治疗和药物开发中。此外，该仪器的开发将使在培养或组织中用光操纵细胞活性成为可能，这种方法可能导致用于调节体内细胞或生理过程的重要新应用。该装置还可能导致药物筛选和开发的新方法，因为它可以提供快速、良好校准和可再现的方法，以单孔或多孔形式将热脉冲递送至细胞或纯化的离子通道蛋白。我们将设计并测试通道激光刺激器，其（A）将直接向细胞的靶膜和通道提供一致的光强度;（B）将使操作员能够精确控制热刺激，从而激活一些（可预测的）通道活动量;以及（C）将使操作员能够通过激光脉冲的功率和脉冲持续时间校准热刺激。我们将根据（1）LASMED开发的红外激光二极管疼痛刺激器的设计和（2）初步测试的结果，设计通道激光刺激器的原型，其参数针对单细胞水平的研究进行了优化。我们将与哺乳动物神经系统中热敏离子通道的分子研究领域的领先科学家进行磋商。验证通道激光刺激器性能的所有必要测试将由该科学家的工作人员在其实验室中进行。