Quantitative Assessment of Murine Tumors with MicroPET.

使用 MicroPET 对小鼠肿瘤进行定量评估。

• 批准号: 6932211
• 负责人: Craig Kendall Abbey
• 金额: $ 18.07万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932211
• 关键词: antineoplastics; breast neoplasms; disease /disorder model; histochemistry /cytochemistry; hyperplasia; laboratory mouse; neoplasm /cancer chemotherapy; neoplastic growth; nonhuman therapy evaluation; positron emission tomography; preneoplastic state; tamoxifen

DESCRIPTION (provided by applicant): This proposal seeks to investigate molecular imaging as an efficient tool to assess the progression of precancerous hyperplastic outgrowths and the initiation of tumors in a mouse model of breast cancer. By mimicking the neoplastic progression of breast cancer in humans, mouse models of this type have tremendous potential as a proving ground for preclinical evaluations of the efficacy of new drugs and other novel therapeutics. However, standard histological techniques for assessing are labor intensive and invasive, and therefore limit the use of mouse models for finding new treatments and diagnostic agents in the effort to fight cancer. Small-animal positron emission tomography (microPET) imaging can make a substantial impact in this area by providing in-vivo functional measurements of disease progression, and by following single animals over multiple time points during this period. In order to realize this potential, accurate quantitative descriptions of disease progression and proliferation must be developed and validated. Here, we hypothesize that quantitative methods applied to in-vivo microPET images can be used to efficaciously track the proliferation of precancerous hyperplastic outgrowths as well as the initiation and growth of cancerous tumors in mice. This hypothesis is addressed through three studies. The first proposes collection of microPET data on realistic tumor phantoms embedded in background activity. Since ground truth information about the tumor phantoms will be known, these data will form an ideal dataset for developing and testing the accuracy of quantitation algorithms. The second study proposes a microPET study of longitudinal disease progression in mice along with some tissue harvesting for histological comparisons. The data from this study will allow for a validation of in-vivo microPET quantitation approaches. It will also form a baseline for disease progression against which future datasets can be compared to determine if a therapeutic effect has been achieved. The third study proposes a study in which a well-known therapeutic agent, tamoxifen, is introduced. The data from this study will allow evaluation of the magnitude and statistical significance of a therapeutic effect.

描述（由申请人提供）： 该建议旨在研究分子成像作为一种有效的工具，以评估癌前增生的发展和肿瘤的启动在小鼠模型的乳腺癌。通过模拟人类乳腺癌的肿瘤进展，这种类型的小鼠模型具有巨大的潜力，可作为新药和其他新型疗法疗效的临床前评价的试验场。然而，用于评估的标准组织学技术是劳动密集型和侵入性的，因此限制了小鼠模型用于寻找新的治疗和诊断剂以对抗癌症的使用。小动物正电子发射断层扫描（microPET）成像可以通过提供疾病进展的体内功能测量，并在此期间的多个时间点跟踪单个动物，在这一领域产生重大影响。为了实现这一潜力，必须开发和验证疾病进展和增殖的准确定量描述。在这里，我们假设应用于体内microPET图像的定量方法可用于有效地跟踪癌前增生的增殖以及小鼠癌性肿瘤的起始和生长。这一假设是通过三项研究。第一个建议收集嵌入背景活动中的真实肿瘤模型的microPET数据。由于关于肿瘤体模的地面实况信息将是已知的，因此这些数据将形成用于开发和测试定量算法的准确性的理想数据集。第二项研究提出了一项小鼠纵向疾病进展的microPET研究，沿着采集了一些组织进行组织学比较。本研究的数据将允许验证体内microPET定量方法。它还将形成疾病进展的基线，未来的数据集可以与之进行比较，以确定是否已经达到治疗效果。第三项研究提出了一项研究，其中一个众所周知的治疗剂，他莫昔芬，介绍。本研究的数据将允许评价治疗效果的程度和统计学显著性。