Quantitative Assessment of Murine Tumors with MicroPET.

使用 MicroPET 对小鼠肿瘤进行定量评估。

• 批准号: 6677249
• 负责人: Craig Kendall Abbey
• 金额: $ 18.07万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6677249
• 关键词: antineoplastics; breast neoplasms; disease /disorder model; histochemistry /cytochemistry; hyperplasia; laboratory mouse; neoplasm /cancer chemotherapy; neoplastic growth; nonhuman therapy evaluation; positron emission tomography; preneoplastic state; tamoxifen

DESCRIPTION (provided by applicant): This proposal seeks to investigate molecular imaging as an efficient tool to assess the progression of precancerous hyperplastic outgrowths and the initiation of tumors in a mouse model of breast cancer. By mimicking the neoplastic progression of breast cancer in humans, mouse models of this type have tremendous potential as a proving ground for preclinical evaluations of the efficacy of new drugs and other novel therapeutics. However, standard histological techniques for assessing are labor intensive and invasive, and therefore limit the use of mouse models for finding new treatments and diagnostic agents in the effort to fight cancer. Small-animal positron emission tomography (microPET) imaging can make a substantial impact in this area by providing in-vivo functional measurements of disease progression, and by following single animals over multiple time points during this period. In order to realize this potential, accurate quantitative descriptions of disease progression and proliferation must be developed and validated. Here, we hypothesize that quantitative methods applied to in-vivo microPET images can be used to efficaciously track the proliferation of precancerous hyperplastic outgrowths as well as the initiation and growth of cancerous tumors in mice. This hypothesis is addressed through three studies. The first proposes collection of microPET data on realistic tumor phantoms embedded in background activity. Since ground truth information about the tumor phantoms will be known, these data will form an ideal dataset for developing and testing the accuracy of quantitation algorithms. The second study proposes a microPET study of longitudinal disease progression in mice along with some tissue harvesting for histological comparisons. The data from this study will allow for a validation of in-vivo microPET quantitation approaches. It will also form a baseline for disease progression against which future datasets can be compared to determine if a therapeutic effect has been achieved. The third study proposes a study in which a well-known therapeutic agent, tamoxifen, is introduced. The data from this study will allow evaluation of the magnitude and statistical significance of a therapeutic effect.

描述(由申请人提供)： 这项建议旨在研究分子成像作为一种有效的工具来评估癌前增生性突起的进展和乳腺癌小鼠模型的肿瘤起始。通过模拟人类乳腺癌的肿瘤进展，这种类型的小鼠模型具有巨大的潜力，可以作为临床前评估新药和其他新疗法有效性的试验场。然而，用于评估的标准组织学技术是劳动密集型和侵入性的，因此限制了在抗击癌症的努力中使用小鼠模型来寻找新的治疗方法和诊断试剂。小动物正电子发射断层扫描(MicroPET)成像通过提供疾病进展的体内功能测量以及在此期间对单个动物的多个时间点进行跟踪，可以在这一领域产生重大影响。为了实现这一潜力，必须开发和验证对疾病进展和扩散的准确定量描述。在这里，我们假设应用于体内microPET图像的定量方法可以有效地跟踪癌前增生性突起的增殖以及小鼠癌症肿瘤的开始和生长。这一假说通过三项研究得到解决。第一个建议收集嵌入在背景活动中的真实肿瘤幻影的微型PET数据。由于有关肿瘤模体的基本事实信息将是已知的，这些数据将形成一个理想的数据集，用于开发和测试定量算法的准确性。第二项研究提出了一项关于小鼠纵向疾病进展的微型PET研究，并采集了一些组织用于组织学比较。这项研究的数据将使体内microPET定量方法的验证成为可能。它还将形成疾病进展的基线，未来的数据集可以与之进行比较，以确定是否已经取得治疗效果。第三项研究提出了一项研究，其中介绍了一种著名的治疗剂--他莫昔芬。这项研究的数据将允许评估治疗效果的大小和统计意义。