REGULATION OF PROLACTIN GENE EXPRESSION

描述：（改编自研究者摘要）：具体目标 包括：1）检测SLB在调控中的作用 催乳素的转录。2)评估的功能意义 Pit-1与CBP的相互作用，通过使用遗传筛选来鉴定 Pit-1 POU结构域中的突变，其破坏与CBP的结合。3)确定是否 cAMP和TRH信号通路导致染色质结构的变化 催乳素基因4)确定是否刺激催乳素转录 涉及转录辅激活因子募集的变化，或 辅阻遏物和组蛋白修饰的变化。5)测试角色， 特异性转录因子、辅激活因子和辅阻遏因子在 组织的染色质结构和转录潜力， 催乳素基因这将涉及使用体内重组酶进行染色质重建。 非洲爪蟾卵母细胞系统以及体外果蝇胚胎系统。

DESCRIPTION: (Adapted from the Investigator's Abstract): The specific aims include: 1) Test the role of SLB, a putative new co-activator in the regulation of prolactin transcription. 2) Evaluate the functional significance of the interaction of Pit-1 with CBP through the use of a genetic screen to identify mutations in the Pit-1 POU domain which disrupt bindind to CBP. 3) Determine if the cAMP and TRH signaling pathways lead to changes in the chromatin structure of the PRL gene. 4) Determine if stimulation of prolactin transcription involves changes in the recruitment of transcriptional co-activators or co-repressors and changes in histone modification. 5) Test the role that specific transcription factors, co-activators and co-repressors play in organizing the chromatin structure and transcriptional potential of the prolactin gene. This will involve chromatin reconstitution using an in vivo Xenopus oocyte system as well in vitro using a Drosophila embryo system.