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Fluorescence Sensor Probe for Glucose Sensing

用于葡萄糖传感的荧光传感器探头

基本信息

批准号：
6788923
负责人：
Ralph Ballerstadt
金额：
$ 10万
依托单位：
BIOTEX, INC.
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-05-01 至 2005-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6788923
关键词：
biomedical equipment development biosensor device biotechnology clinical biomedical equipment computer assisted medical decision making concanavalin A fluorescence glucose implant laboratory rat optics patient monitoring device skin circulation

项目摘要

DESCRIPTION (provided by applicant): Diabetes mellitus is a metabolic disease, which currently afflicts some 16 million in the US and 100 million people worldwide. In the US, diabetes and its associated complications are the seventh leading cause of death. Effective management of diabetes requires regular measurement of blood sugar, and improvements in glucose sensing technology will enable superior care and regulation of diabetic blood sugar. The focus of this research project is to develop and characterize a minimally invasive near-infrared fluorescence affinity sensor designed for transcutaneous glucose monitoring in interstitial fluid in subdermal skin tissue. The concept of the proposed implant device is one of the most promising technologies currently pursued in glucose sensor research. Significant long-term performance tests have been previously conducted by one of the principle investigators demonstrating that the glucose-sensitive protein Concanavalin A, the primary component of the sensor, has the capability to stay functional at body temperature for over one year. These stability test data are pivotal for ensuring reliable and accurate performance of the fluorescence affinity sensor over many months. We plan to develop, build, and test a small subcutaneously implantable sensor that will allow rapid and painless glucose sensing based on fluorescence measurements through the skin. The sensor will be specifically designed to be compatible with skin optics and will be amenable to inexpensive instrumentation. In this work, we will test the sensor in vitro and preliminarily in vivo in a rat model. The anticipated results of this study will be a miniaturized, rugged, biocompatible prototype of the affinity sensor for glucose monitoring that remains functional for at least 4 weeks in the hairless rat model with a low re-calibration frequency of approximately once a week.

描述（由申请人提供）：糖尿病是一种代谢性疾病，目前美国约有1600万人、全球约有1亿人患有糖尿病。在美国，糖尿病及其相关并发症是第七大死亡原因。糖尿病的有效管理需要定期测量血糖，葡萄糖传感技术的改进将使糖尿病血糖的上级护理和调节成为可能。本研究项目的重点是开发和表征一种微创近红外荧光亲和传感器，用于经皮监测皮下皮肤组织间质液中的葡萄糖。所提出的植入装置的概念是葡萄糖传感器研究中目前最有前途的技术之一。一位主要研究者之前已经进行了重要的长期性能测试，证明葡萄糖敏感蛋白伴刀豆球蛋白A（传感器的主要成分）能够在体温下保持功能超过一年。这些稳定性测试数据对于确保荧光亲和传感器在多个月内的可靠和准确性能至关重要。我们计划开发、构建和测试一种小型皮下植入式传感器，该传感器将允许基于通过皮肤的荧光测量的快速无痛葡萄糖感测。该传感器将专门设计为与皮肤光学兼容，并将适用于廉价的仪器。在这项工作中，我们将测试传感器在体外和初步在体内大鼠模型。本研究的预期结果将是用于葡萄糖监测的亲和传感器的小型化、坚固耐用、生物相容性原型，其在无毛大鼠模型中保持功能至少4周，重新校准频率较低，约为每周一次。