VOLATILE MARKERS OF ORAL MALODOR IN THE BREATH

呼吸中口腔恶臭的挥发性标志物

• 批准号: 6788505
• 负责人: Michael Phillips
• 金额: $ 10万
• 依托单位: MENSSANA RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6788505
• 关键词: biomarker; breath composition; breath tests; clinical research; diagnosis design /evaluation; disease /disorder etiology; gas chromatography mass spectrometry; human subject; odors; oral bacteria; oral pharyngeal disorder

DESCRIPTION: The problem: Oral malodor causes much despair, and treatment is frequently empirical and ineffective because the specific cause is not usually known. The basic problem that has retarded progress in the treatment of oral malodor is the lack of an objective method for evaluating the severity of the condition, identifying the specific etiologies, and measuring response to treatment. A new solution to the problem: An advanced new breath test employing gas chromatography and mass spectroscopy (GC/MS) can detect low concentrations of volatile organic compounds (VOCs) in the breath. This breath test could identify the VOCs responsible for oral malodor, and determine whether treatment reduces their concentrations. Experimental plan to address the problem: The intensity of offensiveness of oral malodor will be rated by an organoleptic panel of human "sniffers" in two groups of human volunteers: subjects with oral malodor, and control subjects with no oral malodor. In addition, breath samples from all subjects will be analyzed by GC/MS, in order to identify the VOCs associated with oral malodor. The short-term Aim of the research is to correlate the subjective assessment by trained and calibrated odor judges with the objective results of a breath test, in order to identify the chemical composition of the VOCs responsible for oral malodor. The long-term Aims of the research are: 1) Diagnostic - to correlate specific causes of oral malodor (e.g. bacterial putrefaction on the dorsum of the tongue) with their breath VOC signature; 2) Therapeutic - to determine the effectiveness of treatments for oral malodor by measuring the reduction in the concentrations of breath VOC signatures; 3) Technological - to develop a mobile GC/MS instrument for breath analysis which could be employed in Dental and medical practices for the evaluation and treatment of oral malodor.

产品说明：问题是：口腔恶臭导致很多绝望，治疗往往是经验和无效的，因为具体原因通常是不知道。阻碍口腔恶臭治疗进展的基本问题是缺乏客观的方法来评估病情的严重程度，识别特定的病因，并测量对治疗的反应。 一种新的解决方案：采用气相色谱和质谱（GC/MS）的先进的新呼吸测试可以检测呼吸中低浓度的挥发性有机化合物（VOC）。这种呼吸测试可以识别导致口腔恶臭的VOC，并确定治疗是否会降低其浓度。 解决该问题的实验计划：口腔恶臭的攻击性强度将由两组人类志愿者中的人类“嗅探者”的感官小组评定：口腔恶臭的受试者和没有口腔恶臭的对照受试者。此外，将通过GC/MS分析所有受试者的呼吸样本，以确定与口腔恶臭相关的VOC。 研究的短期目标是将经过培训和校准的气味判断者的主观评估与呼吸测试的客观结果相关联，以确定导致口腔恶臭的VOC的化学成分。研究的长期目标是：1）诊断-将口腔恶臭的具体原因联系起来2）治疗性-通过测量呼吸VOC特征的浓度的降低来确定口腔恶臭的治疗的有效性; 3）技术方面-发展一套移动的气相色谱/质谱分析呼气的仪器，可应用于牙科及医疗工作，以评估及治疗口腔异味。