Mechanisms of fibrin action in multiple sclerosis

纤维蛋白在多发性硬化症中的作用机制

• 批准号: 7255825
• 负责人: Katerina Akassoglou
• 金额: $ 24.73万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7255825
• 关键词: Alzheimer' s Disease; Ancrod; Animal Model; Anticoagulants; Anticoagulation; Applications Grants; Binding; Binding Sites; Blood - brain barrier anatomy; Blood coagulation; Cell Surface Receptors; Cells; Cessation of life; Chronic; Coagulants; Data; Demyelinations; Deposition; Disease; Disruption; Doctor of Philosophy; Encephalomyelitis; Endopeptidases; Event; Experimental Autoimmune Encephalomyelitis; Fibrin; Fibrin split products; Fibrinogen; Genes; Genetic; Goals; Hemorrhage; Hemostatic Agents; ITGAM gene; In Vitro; Inflammatory; Inflammatory Response; Injury; Knock-in Mouse; Knock-out; Low-Molecular-Weight Heparin; Macrophage-1 Antigen; Mediating; Microglia; Modeling; Molecular; Multiple Sclerosis; Mus; Mutant Strains Mice; Mutation; Natural regeneration; Nervous system structure; Neurodegenerative Disorders; Neurologic; Neurons; None or Not Applicable; Paralysed; Pathogenesis; Pathology; Peptide Hydrolases; Peptides; Personal Satisfaction; Phagocytosis; Play; Process; Receptor Signaling; Relapse; Research Personnel; Risk; Role; Signal Pathway; Signal Transduction; Site; Snake Venoms; System; Testing; Therapeutic; Therapeutic Effect; Tissues; Treatment Efficacy; Warfarin; Work; cytokine; design; fibrin receptor; in vivo; inhibitor/antagonist; neuroinflammation; novel therapeutics; receptor; repaired

DESCRIPTION (provided by applicant): The widespread deposition of fibrin and fibrin degradation products (FDPs) within the nervous system is well documented in demyelinating plaques in Multiple Sclerosis (MS). Given that fibrin, FDPs and their cell surface receptors play a role in both the inflammatory response and tissue remodeling/repair, they are prime candidates to be critical determinants of inflammatory demyelination. Our major hypothesis is that fibrin utilizes receptors of nervous system cells to exert deleterious effects in nervous system pathology. Our preliminary data demonstrate that: 1. Pharmacologic depletion of fibrin reverses relapsing paralysis and ameliorates inflammatory demyelination in autoimmune encephalomyelitis (EAE); 2. Fibrin induces microglia activation both in vivo and in vitro; 3. Blocking the Mac-1 (CD11b/CD18) fibrin receptor ameliorates fibrin-induced microglia activation in vitro. Our ultimate goal is to design a novel therapeutic approach for fibrin depletion with potential application in MS and other neurologic diseases associated with fibrin deposition. In this grant proposal we will determine the impact of genetic depletion of fibrinogen or genetic elimination of the fibrinogen Mac-1 binding site in animal models for MS (Aim 1). We will examine the involvement of fibrin/Mac-1 interactions in functions of microglia activation, such as proliferation, phagocytosis and cytokine release (Aims 2 and 3). The therapeutic efficacy of 2 known anticoagulants, as well as the efficacy of a specific fibrin/Mac-1 inhibitor will be assessed in 2 animal models of MS (Aim 4). The proposed studies will provide a cellular and molecular definition of the role of hemostatic factors in the pathogenesis of inflammatory demyelination and will determine the therapeutic efficacy of anticoagulants in animal models for MS. Identifying the receptors of fibrin actions in the nervous system could ultimately illuminate new therapeutic strategies for the treatment of MS.

描述（由申请方提供）：在多发性硬化症（MS）的脱髓鞘斑块中，充分记录了神经系统内纤维蛋白和纤维蛋白降解产物（FDP）的广泛沉积。鉴于纤维蛋白、FDP及其细胞表面受体在炎症反应和组织重塑/修复中起作用，它们是炎症性脱髓鞘的关键决定因素的主要候选者。我们的主要假设是，纤维蛋白利用神经系统细胞的受体在神经系统病理中发挥有害作用。我们的初步数据表明：1。纤维蛋白的药理学消耗逆转复发性麻痹并改善自身免疫性脑脊髓炎（EAE）中的炎性脱髓鞘; 2.在体内和体外，fietamine均诱导小胶质细胞活化; 3.阻断Mac-1（CD 11b/CD 18）纤维蛋白受体可改善纤维蛋白诱导的小胶质细胞体外活化。我们的最终目标是设计一种新的治疗方法，纤维蛋白消耗与MS和其他神经系统疾病与纤维蛋白沉积的潜在应用。在本资助提案中，我们将确定MS动物模型中纤维蛋白原遗传耗竭或纤维蛋白原Mac-1结合位点遗传消除的影响（目的1）。我们将研究纤维蛋白/Mac-1相互作用在小胶质细胞活化功能中的参与，如增殖、吞噬作用和细胞因子释放（目的2和3）。将在2种MS动物模型中评估2种已知抗凝剂的疗效以及特异性纤维蛋白/Mac-1抑制剂的疗效（目的4）。拟议的研究将提供止血因子在炎症性脱髓鞘发病机制中的作用的细胞和分子定义，并将确定抗凝血剂在MS动物模型中的治疗效果。确定神经系统中纤维蛋白作用的受体最终可能阐明治疗MS的新治疗策略。