Physiology Core

生理学核心（C. Rosen，核心主任） 生理学核心的长期目标是提供高质量的体内和体外代谢表型分析 为 COBRE 项目、其他缅因州健康研究人员和外部研究人员提供服务。第一阶段 成就包括通过收购 8 G2 扩展和升级我们的代谢表型 HR E-Mitter 遥测系统、Seahorse XF96 分析仪、Cytation I 细胞成像仪、Faxitron UltraFocus 根据用户的需求提供 DXA X 射线机和 Sable Systems 呼吸测定跑步机。我们成功地 支持我们的 COBRE 研究人员和非 COBRE 机构教职人员、研究人员和实习生，从而 满足我们支持资助拨款申请和出版物的目标指标。在过去的五年里， 所有四个原始项目负责人都获得了独立的 R01 资助，并且是 Physiology 的频繁用户 核心，用于实验服务或咨询罗森博士。他们的研究包括细胞分析 生物能量学、身体成分以及代谢和行为研究。此外，我们还成功地 通过与新英格兰大学 Solarea Bio 的合作项目增加外部利用率 （马萨诸塞州剑桥）、西奈山医学院、塔夫茨大学和康奈尔大学。这些导致了稳定的 年度服务费收入的增加对于可持续发展至关重要。生理学核心的使用是 在从 NIA 到 Drs 的多 PI U19 资助中发挥了重要作用。 Zaidi（西奈山）和 Rosen（生理学核心） 导演）。发表于 PNAS、J Biol Chem、J Clinical Investigation 和 JCI Insight、eLife、Cancer Research、 《细胞代谢》、《自然通讯》和《细胞报告》是该核心实验的直接结果。 重要的是，生理学核心支持年轻研究者的培训，包括成功的 为 F31 和 F32 提供资金。大多数试点项目获奖者都使用了该核心的功能之一，并且 向本科生提供了与核心设备新技术相关的教育计划 以及研究生和博士后研究员。第二阶段为生理学提供了新的机遇 核心在于新技术、扩大现有设备的使用和更多的培训机会。在 此外，我们最近获得了 COBRE 设备补充品，以扩大代谢数量 笼子并在系统内集成一个温控培养箱。因此，在第二阶段，我们扩大了 我们的设备和能力可以满足代谢和行为表型分析的高需求，并且还将 为热中性和低温下的体内研究提供新服务。我们将维持我们的 高度利用高级细胞和线粒体生物能分析服务，此外还提供 研究设计和实施以及数据分析方面的专家协助。成功的领导， 该核心的管理和运作原则将保持不变，并科学地支持该项目 领导博士。 Lynes、Li 和 Agarwal，以及其他缅因州卫生局和外部调查人员。

Physiology Core (C. Rosen, Core Director) The long-term goal of the Physiology Core is to provide high quality in vivo and in vitro metabolic phenotyping services for COBRE projects, other MaineHealth researchers, and outside investigators. Phase I accomplishments include expanding and upgrading our metabolic phenotyping through the acquisition of 8 G2 HR E-Mitter telemetry systems, a Seahorse XF96 analyzer, a Cytation I cell imager, a Faxitron UltraFocus DXA X-ray machine and a Sable Systems respirometry treadmill, based on our users’ needs. We successfully supported our COBRE investigators and non-COBRE institutional faculty, research staff, and trainees, thereby meeting our targeted metrics for support of funded grant applications and publications. Over the last five years, all four original project leads received independent R01 funding, and were frequent users of the Physiology Core, for experimental services or consultation with Dr. Rosen. Their studies included analysis of cellular bioenergetics, body composition, and metabolic and behavioral studies. Moreover, we have successfully increased outside utilization through collaborative projects with the University of New England, Solarea Bio (Cambridge, MA), Mt. Sinai School of Medicine, Tufts and Cornell Universities. These resulted in steady increases in annual service fee revenue that is integral for sustainability. Use of the Physiology Core was instrumental in the funding of a multi-PI U19 from NIA to Drs. Zaidi (Mt. Sinai) and Rosen (Physiology Core Director). Publications in PNAS, J Biol Chem, J Clinical Investigation and JCI Insight, eLife, Cancer Research, Cell Metabolism, Nature Communications, and Cell Reports were direct results of experiments from this Core. Importantly, the Physiology Core was supportive of young investigators in training, including the successful funding of an F31 and an F32. Most of the pilot project awardees used one of the features of this Core, and educational programs related to new technical aspects for Core equipment were provided to undergraduate and graduate students as well as postdoctoral fellows. Phase II offers new opportunities for the Physiology Core in respect to novel technologies, expanded use of current equipment and greater training opportunities. In addition, we were recently awarded a COBRE equipment supplement to expand the number of metabolic cages and integrate a temperature-controlled incubator within the system. Thus in Phase II, we have expanded our equipment and capacity to meet the high demand for metabolic and behavioral phenotyping, and will also provide new services for in vivo studies at thermoneutrality and at cold temperatures. We will maintain our highly utilized services for advanced cellular and mitochondrial bioenergetic analysis, in addition to providing expert assistance in research study design and implementation and data analysis. The successful leadership, management, and operational principles of this Core will remain in place, and scientifically support the project leads Drs. Lynes, Li, and Agarwal, and other MaineHealth and external investigators.