Physiology Core

生理学核心

基本信息

批准号：
10711694
负责人：
CLIFFORD JAMES ROSEN
金额：
$ 31.05万
依托单位：
MAINEHEALTH
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2028-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10711694
关键词：
3-Dimensional Address Age Animal Model Animals Applications Grants Award Behavior Biochemical Pathway Bioenergetics Body Composition Body Temperature Body Weight Cell physiology Cells Cellular Metabolic Process Centers of Research Excellence Communication Consultations Controlled Environment Cost Analysis Counseling Coupled Data Data Analyses Data Set Digital X-Ray Dual-Energy X-Ray Absorptiometry Education Energy Metabolism Equipment Exercise Experimental Designs Faculty Fee-for-Service Plans Food Funding Future Generations Genus Hippocampus Glycolysis Goals Grant Heart Rate Human Resources Image In Vitro Incubators Institution Leadership Martes zibellina Measures Mesenchymal Metabolic Mitochondria Modeling Mus Nature New England Output Phase Physical activity Physiological Physiology Pilot Projects Positioning Attribute Postdoctoral Fellow Productivity Publications Quality Control Reader Reporting Research Research Design Research Institute Research Personnel Resources Respiration Roentgen Rays Services Site Spirometry Structure Suggestion System Technology Telemetry Temperature Time Tissues Training Universities Water consumption anticancer research behavioral phenotyping behavioral study clinical investigation cold temperature cytate experimental study flexibility food consumption gas analyzer graduate student human disease human model imager in vivo insight medical schools meetings metabolic abnormality assessment metabolic phenotype mouse model mutant neuroregulation new technology novel oxidation programs research study response service utilization sex success tissue culture training opportunity treadmill undergraduate student

项目摘要

Physiology Core (C. Rosen, Core Director) The long-term goal of the Physiology Core is to provide high quality in vivo and in vitro metabolic phenotyping services for COBRE projects, other MaineHealth researchers, and outside investigators. Phase I accomplishments include expanding and upgrading our metabolic phenotyping through the acquisition of 8 G2 HR E-Mitter telemetry systems, a Seahorse XF96 analyzer, a Cytation I cell imager, a Faxitron UltraFocus DXA X-ray machine and a Sable Systems respirometry treadmill, based on our users’ needs. We successfully supported our COBRE investigators and non-COBRE institutional faculty, research staff, and trainees, thereby meeting our targeted metrics for support of funded grant applications and publications. Over the last five years, all four original project leads received independent R01 funding, and were frequent users of the Physiology Core, for experimental services or consultation with Dr. Rosen. Their studies included analysis of cellular bioenergetics, body composition, and metabolic and behavioral studies. Moreover, we have successfully increased outside utilization through collaborative projects with the University of New England, Solarea Bio (Cambridge, MA), Mt. Sinai School of Medicine, Tufts and Cornell Universities. These resulted in steady increases in annual service fee revenue that is integral for sustainability. Use of the Physiology Core was instrumental in the funding of a multi-PI U19 from NIA to Drs. Zaidi (Mt. Sinai) and Rosen (Physiology Core Director). Publications in PNAS, J Biol Chem, J Clinical Investigation and JCI Insight, eLife, Cancer Research, Cell Metabolism, Nature Communications, and Cell Reports were direct results of experiments from this Core. Importantly, the Physiology Core was supportive of young investigators in training, including the successful funding of an F31 and an F32. Most of the pilot project awardees used one of the features of this Core, and educational programs related to new technical aspects for Core equipment were provided to undergraduate and graduate students as well as postdoctoral fellows. Phase II offers new opportunities for the Physiology Core in respect to novel technologies, expanded use of current equipment and greater training opportunities. In addition, we were recently awarded a COBRE equipment supplement to expand the number of metabolic cages and integrate a temperature-controlled incubator within the system. Thus in Phase II, we have expanded our equipment and capacity to meet the high demand for metabolic and behavioral phenotyping, and will also provide new services for in vivo studies at thermoneutrality and at cold temperatures. We will maintain our highly utilized services for advanced cellular and mitochondrial bioenergetic analysis, in addition to providing expert assistance in research study design and implementation and data analysis. The successful leadership, management, and operational principles of this Core will remain in place, and scientifically support the project leads Drs. Lynes, Li, and Agarwal, and other MaineHealth and external investigators.

生理学核心（C. Rosen，核心主任）生理学核心的长期目标是提供体内和体外代谢表型的高质量为山羊皮项目，其他Mainhealth研究人员和外部研究人员提供服务。第一阶段成就包括通过获取8 G2扩大和升级我们的代谢表型 HR e-Mitter遥测系统，Seahorse XF96分析仪，一个细胞I细胞成像仪，Faxitron Ultrafocus DXA X射线机和Sable Systems呼吸测定治疗机，根据我们的用户需求。我们成功支持我们的毛茸茸的调查员和非近代机构的教职员工，研究人员和受训者满足我们的目标指标，以支持资助的赠款申请和出版物。在过去的五年中，所有四个原始项目领导者都获得了独立的R01资金，并且经常是生理学的使用者核心，用于实验服务或与Rosen博士进行咨询。他们的研究包括分析细胞生物能学，身体组成以及代谢和行为研究。而且，我们已经成功通过与新英格兰大学Solarea Bio的协作项目增加了外部利用率（马萨诸塞州剑桥），西奈山医学院，塔夫茨和康奈尔大学。这些导致稳定每年服务费收入增加，这是可持续性不可或缺的。生理核心的使用是从NIA到DRS的多PI U19的资助。 Zaidi（西奈山）和Rosen（生理学核心导演）。 PNAS，J Biol Chem，J临床研究和JCI Insight的出版物，Elife，癌症研究，细胞代谢，自然通信和细胞报告是该核心实验的直接结果。重要的是，生理学核心支持年轻研究人员的培训，包括成功 F31和F32的资金。大多数飞行员项目获奖者都使用了该核心的功能之一，提供了与核心设备新技术方面有关的教育计划以及研究生以及博士后研究员。第二阶段为生理提供了新的机会关于新技术，扩大当前设备的使用以及更大的培训机会的核心。在此外，我们最近被授予了毛绒设备补充剂，以扩大代谢数量笼子并集成了系统内温度控制的孵化器。在第二阶段，我们已经扩展了我们满足对代谢和行为表型的高需求的设备和能力，也将在热温和寒冷温度下为体内研究提供新服务。我们将维护我们的高级使用的服务用于高级蜂窝和线粒体生物能分析，此外还提供研究研究设计，实施和数据分析方面的专家协助。成功的领导管理和该核心的运营原理将保持到位，并科学支持该项目领导博士。 Lynes，Li和Agarwal以及其他Mainhealth和外部研究人员。