Animal Models and Histology Core

动物模型和组织学核心

• 批准号: 10715404
• 负责人: Karen Y Stokes
• 金额: $ 42.64万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10715404
• 关键词: Address; Animal Model; Animals; Area; Awareness; Biology; Blood Pressure; Blood Vessels; Blood flow; Blood gas; Breeding; Calorimetry; Cardiac; Cardiovascular Diseases; Cardiovascular system; Cause of Death; Centers of Research Excellence; Charge; Chemistry; Clinical Chemistry; Collaborations; Complement; Death Rate; Development; Disease model; Equipment; Exercise; Faculty; Feedback; Frequencies; Funding; Funding Agency; Future; Future Teacher; Generations; Genotype; Goals; Grant; Growth; Heart; Histologic; Histology; Human Resources; Immunohistochemistry; Implant; Income; Infrastructure; Institution; Intervention; Lipids; Louisiana; Measurement; Measures; Mentors; Metabolic; Modality; Modeling; Modification; Mus; Operative Surgical Procedures; Organ; Oxidation-Reduction; Pathologic; Pathology; Pathway interactions; Phase; Phenotype; Pilot Projects; Positioning Attribute; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Price; Protein Structure Initiative; Rattus; Regulation; Research; Research Personnel; Rodent; Rodent Model; Role; Running; Schools; Science; Services; Signal Transduction; Source; Stains; Study models; Surgical Models; Surveys; System; Techniques; Technology; Telemetry; Temperature; Testing; Time; Tissues; Training; United States; United States National Institutes of Health; VO2max; Wages; cost; exercise regimen; experimental study; heart function; implantation; improved; in vivo Model; instrument; laboratory experience; member; non-invasive imaging; pressure; programs; skills; tissue processing; treadmill; ultrasound

PROJECT ABSTRACT The goal of the Center for Redox Biology and Cardiovascular Disease is to improve the infrastructure for junior and senior investigators and to provide mentoring for the junior faculty members so that they become more competitive for independent NIH funding. The Animal Models & Histology Core B, led by Dr Karen Stokes, provides services to both the COBRE members and others at our institute, with 28 users thus far in Phase 1. Core services are also available to nearby institutes at which our cardiovascular researchers have collaborations. A needs survey of faculty identified some key new equipment/services that faculty require for growth of their research programs, with an anticipated increase to 32 users in Phase 2. Dr Hugh Price will continue to lead the Histology and Genotyping Subcore. This will build upon current existing services that provide tissue processing, cardiovascular-related stains, and a genotyping service, to add an immunohistochemistry service (with an automated stainer to help maintain robust histology and genotyping services). The Cardiovascular Phenotyping Subcore, led by Dr Shenu Bhuiyan, will continue to provide capabilities to characterize the disease models. Current equipment includes a Peri-med PSI system and Periflux 5000 to non-invasively image and measure blood flow, a VisualSonics Vevo 3100 Ultra High Frequency Ultrasound to study cardiac and vascular function, an AD Instruments MPVS-Ultra single segment Pressure-Volume Unit for in depth cardiac function measurements, two TSE telemetry systems for continuous recording of blood pressure, activity, temperature, and biopotentials, and an OxyletPro System with a single lane mouse calorimetry treadmill for VO2 max determination during forced exercise. In Phase 2, the telemetry and OxyletPro systems will be complemented by the addition of a CODA High Throughput Noninvasive blood pressure system, for low-cost simultaneous blood pressure measurements in 8 mice/rats, and a 5-lane treadmill for higher throughput exercise regimens respectively, and a blood gas/basic clinical chemistry analyzer will be added. This subcore will also facilitate more in depth clinical chemistry analysis (e.g. organ specific or lipid panels) by external sources. Full-time research technicians have been well-trained on all current modalities and will be trained on future services, so that they can continue to either provide the services, or train lab personnel to perform their experiments using the available technologies. The CCDS Surgical Core will continue to liaise with the CoBRE Core B to implant telemetry probes, and to set up surgical rodent models that undergo subsequent phenotyping in Core B. Taken together, the services offered by the Animal Models and Histology Core will help PIs generate animal models suitable for their research questions, and provide cutting edge phenotypic and pathological analysis to maximize the competitiveness of our junior faculty for future funding in the area of redox regulation of cardiovascular disease.

项目摘要 氧化还原生物学和心血管疾病中心的目标是改善初级保健的基础设施， 和高级研究人员，并为初级教师提供指导，使他们成为更多的 竞争独立的NIH资金。由凯伦·斯托克斯博士领导的动物模型和组织学核心B， 为COBRE成员和我们研究所的其他人提供服务，到目前为止，第一阶段有28个用户。 我们的心血管研究人员合作的附近机构也可以提供核心服务。 对教师进行的需求调查确定了教师发展其业务所需的一些关键新设备/服务。 研究计划，预计在第二阶段将增加到32个用户。休·普莱斯博士将继续领导 组织学和基因分型亚核心。这将建立在目前现有的提供组织处理的服务基础上， 心血管相关染色和基因分型服务，以增加免疫组织化学服务（ 自动染色机，以帮助维持稳健的组织学和基因分型服务）。心血管表型 由Shenu Bhuiyan博士领导的Subcore将继续提供表征疾病模型的能力。 目前的设备包括Perimed PSI系统和Periflux 5000，用于非侵入性成像和测量 血流，VisualSonics Vevo 3100超高频超声，用于研究心脏和血管功能， AD Instruments MPVS-Ultra单段压力-容积装置，用于深入心脏功能 测量，两个TSE遥测系统，用于连续记录血压，活动，温度， 和生物电位，以及OxyletPro系统，配有单通道小鼠量热跑步机，用于VO 2 max 在强制锻炼中的决心。在第2阶段，将补充遥测和OxyletPro系统 通过增加CODA高重复性无创血压系统，实现低成本的同时采血 在8只小鼠/大鼠中进行压力测量，以及用于更高通量运动方案的5车道跑步机 并增加血气/基础临床生化分析仪。该子核心还将促进 通过外部来源进行更深入的临床化学分析（例如，器官特异性或脂质检测）。全职 研究技术人员已经接受了关于所有现有模式的良好培训，并将接受关于未来服务的培训， 他们可以继续提供服务，或培训实验室人员使用 可用的技术。CCDS外科核心将继续与CoBRE核心B联系， 遥测探针，并建立外科啮齿动物模型，随后在核心B中进行表型分析。采取 动物模型和组织学中心共同提供的服务将帮助PI生成动物模型 适合他们的研究问题，并提供尖端的表型和病理分析，以最大限度地提高 我们的初级教师在心血管氧化还原调节领域的未来资金的竞争力 疾病