Roles of Recoding and Transcription Slippage

重新编码和转录滑动的作用

• 批准号: 7268941
• 负责人: RAYMOND F GESTELAND
• 金额: $ 28.35万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7268941
• 关键词: Amino Acids; DNA; DNA-Directed RNA Polymerase; Fungal Genome; Gene Expression; Genes; Genetic Transcription; Goals; Human; Human Genome; Mammals; Messenger RNA; Minority; Organism; Protein Analysis; Protein Biosynthesis; Proteins; Reading Frames; Ribosomes; Role; Running; Selenocysteine; Sequence Analysis; Signal Transduction; Site; Standards of Weights and Measures; Terminator Codon; Testing; Vertebrates; base; comparative; programs

DESCRIPTION (provided by applicant): The expression of a minority of genes involves a substantial proportion of ribosomes shifting reading frame at specific site(s). This frameshifting occurs at shift-prone sites and is commonly greatly stimulated by signals embedded elsewhere in the mRNA, often nearby; i.e. it is programmed. It is proposed to computationally identify candidates for utilized frameshifting in vertebrates especially mammals, and experimentally test them. The goal is to determine the roles of this type of recoding in mammals. Another type of recoding is the dynamic programming of stop codons. Cases where the redefinition involves insertion of a standard amino acid, rather than selenocysteine, will also be similarly investigated. Finally, the extent and function of slippage of RNA polymerase slippage at runs of repeat bases will be assessed. Some occurrences of translational and transcriptional slippage-prone sequences will not be utilized for gene expression and their consequences will be solely errors. The extent of these errors will be investigated.

描述（由申请人提供）：少数基因的表达涉及在特定位点转移阅读框的相当一部分。这种帧速率发生在易转发的位置，通常受到mRNA其他地方（通常在附近）的信号的极大刺激。即它是编程的。提议在计算上识别用于在脊椎动物（尤其是哺乳动物）中使用的框架的候选者，并在实验中测试它们。目的是确定这种类型的哺乳动物中这种再生的作用。重新编码的另一种类型是终止密码子的动态编程。重新定义涉及插入标准氨基酸而不是硒代半胱氨酸的情况也将被类似地研究。最后，将评估RNA聚合酶滑移的滑倒的程度和功能，将评估重复碱基的运行。转移和转录易受滑移序列的某些出现将不会用于基因表达，它们的后果将完全是错误。这些错误的程度将被研究。

项目成果

The potential role of ribosomal frameshifting in generating aberrant proteins implicated in neurodegenerative diseases.

• DOI: 10.1261/rna.84406
• 发表时间: 2006-07
• 期刊: RNA
• 影响因子: 4.5
• 作者: N. Wills;J. F. Atkins