CENTER FOR HUMAN GENOME RESEARCH

人类基因组研究中心

• 批准号: 2674193
• 负责人: RAYMOND F GESTELAND
• 金额: $ 223.21万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2674193
• 关键词: biomedical facility; genome; nucleic acid sequence

The Utah Center for Human Genome Research is an interdisciplinary group of biologist, engineers and computer scientists that is developing large scale, directed DNA sequencing technology building on accomplishments of the first funding period. The core technology for sequencing is transposon based to organize the input DNA, with automated multiplex sequencing using a new, automated probing instrument. A novel fluorogenic detection system provides on-line capture of sequence information with wholly integrated computer control and base calling. By year five the sequencing system should sustain a rate of 14mb per year of finished sequence. Sequencing of the entire human chromosome 17 will be initiated. This core proving technology will also be developed for rapid genotyping and sequence comparisons as part of a technology access component. Also the interrelated technology of capillary electrophoresis for rapid sequencing and genotyping will be pursued using a fluidics based sample handling. Core components include a program in ethical, legal and social issues.

犹他州人类基因组研究中心是一个跨学科的小组 生物学家，工程师和计算机科学家正在发展大型 比例，定向DNA测序技术建设 第一个资金期。 测序的核心技术是转座子 基于组织输入DNA，使用自动多重测序使用 一种新的自动探测器。 一种新型的荧光检测系统 提供全面集成的序列信息的在线捕获 计算机控制和基础调用。 到五年级，测序系统 应维持每年成品序列的14MB速度。 测序 将启动整个人类17号染色​​体。 该核心证明的技术也将用于快速基因分型 作为技术访问组件的一部分，序列比较。 还 毛细管电泳的相互关联技术用于快速 测序和基因分型将使用基于流体的样品进行 处理。 核心组成部分包括道德，法律和社会的计划 问题。

项目成果

Should we expect substitution rate to depend on population size?

我们是否应该预期替代率取决于人口规模？

• DOI: 10.1093/genetics/150.2.911
• 发表时间: 1998
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Cherry,JL