Developing a Transposon-based Toolkit for Genetic Analysis of Malaria Parasites

开发基于转座子的疟原虫遗传分析工具包

• 批准号: 7267900
• 负责人: John H Adams
• 金额: $ 4.04万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2007-10-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7267900
• 关键词: Africa South of the Sahara; Biology; Cessation of life; Child; Clinical; Codon Nucleotides; Communities; Condition; Development; Elements; Engineering; Epitopes; Exons; Genes; Genetic; Genetic Screening; Genome; Genomics; Goals; Green Fluorescent Proteins; Human; Infection; Insecta; Insertional Mutagenesis; Laboratories; Malaria; Measures; Methods; Molecular; Mutagenesis; Organism; Parasites; Pharmaceutical Preparations; Phenotype; Plasmids; Plasmodium; Plasmodium falciparum; Protocols documentation; Reporter; Research; Research Personnel; Rodent; Rodent Model; Screening procedure; Staging; System; Technology; Testing; Time; Transfection; Transposase; Vaccines; base; c-myc Genes; design; functional genomics; genetic analysis; high throughput screening; high throughput technology; promoter; tool; transgene expression; vector

DESCRIPTION (provided by applicant): Malaria is a leading cause of human death and illness, causing each year 400-600 million cases of clinical malaria and 2-3 million deaths. Traditional measures to control and cure malaria are becoming increasingly ineffective, and there is an urgent need for the development of new drugs and vaccines. Genomic studies and other hi-tech advances have produced a wealth of information about malaria parasites; yet using this information for functional analysis of the Plasmodium genome is hindered by a limited capability to genetically manipulate malaria parasites. We are developing technology for high throughput whole-genome mutagenesis screening of malaria parasites, using an efficient transposon-based method for parasite transformation. We propose developing this system for large-scale transposon mutagenesis of Plasmodium falciparum with the long-term goal of enhancing our understanding of the genetic basis of the biology of the malaria parasite, and greatly accelerating efforts to develop new therapies.

描述（由申请人提供）：疟疾是人类死亡和疾病的主要原因，每年造成4 - 6亿例临床疟疾病例和2-3百万例死亡。控制和治疗疟疾的传统措施越来越无效，迫切需要开发新的药物和疫苗。基因组学研究和其他高科技进步已经产生了丰富的信息疟原虫，但利用这些信息的疟原虫基因组的功能分析是由一个有限的能力，遗传操纵疟原虫的阻碍。我们正在开发疟疾寄生虫的高通量全基因组诱变筛选技术，使用一种有效的基于转座子的寄生虫转化方法。我们建议开发这种系统，用于恶性疟原虫的大规模转座子诱变，其长期目标是提高我们对疟原虫生物学遗传基础的理解，并大大加快开发新疗法的努力。