MOLECULAR BIOLOGY AND TISSUE ENGINEERING CORE

分子生物学和组织工程核心

• 批准号: 7561486
• 负责人: KASTURI RANGANNA
• 金额: $ 20.96万
• 依托单位: TEXAS SOUTHERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561486
• 关键词: 3-Dimensional; Animals; Architecture; Area; Biological; Biological Models; Biomedical Research; Cell Communication; Cells; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Condition; Core Facility; Cultured Cells; Development; Disease; Drug Design; Faculty; Fostering; Funding; Gene Expression; Gene Proteins; Genomics; Goals; Grant; Hand; Health; Human; In Vitro; Institution; Investigation; Laboratories; Molecular; Molecular Biology; Organism; Pattern; Play; Purpose; Research; Research Activity; Research Infrastructure; Research Personnel; Resources; Role; Services; Simulate; Source; Technology; Texas; Tissue Engineering; Tissue Grafts; Tissue Model; Tissues; Training; United States National Institutes of Health; Universities; anticancer research; in vivo; instrumentation; laboratory facility; novel; protein expression; receptor expression; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The existing Molecular Biology and Tissue Culture Laboratory (MBL/TCL) core unit has played a key role in fostering research activities of various faculty and research investigators at Texas Southern University (TSU). The main purpose of the MBL/TCL was to enhance research investigations at the cellular and molecular level by providing resources and technical support, as well as hands-on-training in these areas. In order to increase competitiveness in the biomedical research arena, support new academic goals, and conform to post-genomic technological advances, the existing MBL/TCL requires continued support to enhance and expand the available resources. Enhancement of this facility will enable the MBL/TCL core to provide critical post-genomic technical and instrumentation services. The 3-dimensional (3-D) tissue culture technology has recently revolutionized biomedical research, particularly in the areas of tissue grafting, cancer research, and drug design. This 3-D tissue culture technology provides a novel in vitro tissue model system for understanding cell-cell interactions with respect to gene, protein, and receptor expression. The need for developing such a capability is timely as more researchers are increasingly turning to 3-D cell culture since patterns of gene expression and other biological activities more closely mirror what happens in a living system. Recent developments in tissue engineering clearly suggest that there are numerous differences between a 2-D flat layer of cells and a complex 3-D tissue. Expansion of the MBL/TCL facility involves the addition of a new Tissue Engineering (TE) component. This component will provide the training, technical support, and infrastructure to grow the complex 3-D tissue-equivalent architecture, which simulates in vivo, conditions for studying gene/protein expression and cell-cell interactions in human health and disease states. Therefore, the purpose of this project is to maintain funds to enhance, and expand the existing MBL/TCL core facility with a new TE technology to form the Molecular Biology and Tissue Engineering (MBTE) Core Facility. Integration of a tissue engineering component will complement the existing MBL/TCL core facility and strengthen its service capability to conduct advanced in vitro studies before proceeding to in vivo studies using whole animals.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 现有的分子生物学和组织培养实验室 (MBL/TCL) 核心单位在促进德克萨斯南方大学 (TSU) 各教师和研究人员的研究活动方面发挥了关键作用。 MBL/TCL 的主要目的是通过提供资源和技术支持以及这些领域的实践培训来加强细胞和分子水平的研究调查。为了提高生物医学研究领域的竞争力，支持新的学术目标，并顺应后基因组技术的进步，现有的 MBL/TCL 需要持续的支持，以增强和扩大可用资源。该设施的增强将使 MBL/TCL 核心能够提供关键的后基因组技术和仪器服务。 3 维 (3-D) 组织培养技术最近彻底改变了生物医学研究，特别是在组织移植、癌症研究和药物设计领域。这种 3D 组织培养技术提供了一种新颖的体外组织模型系统，用于了解基因、蛋白质和受体表达方面的细胞间相互作用。随着越来越多的研究人员越来越多地转向 3D 细胞培养，因为基因表达和其他生物活动的模式更接近地反映生命系统中发生的情况，开发这种能力的需求是及时的。组织工程的最新发展清楚地表明，2D 平坦细胞层和复杂的 3D 组织之间存在许多差异。 MBL/TCL 设施的扩建涉及增加新的组织工程 (TE) 组件。该组件将提供培训、技术支持和基础设施来培养复杂的 3D 组织等效架构，该架构模拟体内研究人类健康和疾病状态下基因/蛋白质表达和细胞间相互作用的条件。因此，该项目的目的是维持资金，利用新的 TE 技术增强和扩展现有的 MBL/TCL 核心设施，形成分子生物学和组织工程 (MBTE) 核心设施。组织工程组件的整合将补充现有的 MBL/TCL 核心设施，并增强其在使用整只动物进行体内研究之前进行先进体外研究的服务能力。