ACT 1: PROJ 2: TARGETED HIGH-FREQUENCY ULTRASOUND-CONTRAST AGENT IMAGING

行动 1：项目 2：定向高频超声造影剂成像

• 批准号: 7561547
• 负责人: John A Allen
• 金额: $ 16.58万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561547
• 关键词: Acute; Binding; Biological Process; Blood; Blood Vessels; Cardiovascular Diseases; Cause of Death; Cells; Computer Retrieval of Information on Scientific Projects Database; Condition; Contracts; Contrast Media; Detection; Development; Diagnostic Imaging; Encapsulated; Equilibrium; Fostering; Foundations; Frequencies; Functional disorder; Funding; Grant; Hawaii; Image; Individual; Institution; Leukocytes; Ligands; Methods; Molecular; Physiological; Relative (related person); Research; Research Personnel; Resources; Site; Source; Staging; Techniques; Tissues; Translations; Ultrasonography; United States; United States National Institutes of Health; cost; improved; molecular imaging; novel; portability; radius bone structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Cardiovascular disease is the leading cause of death in the United States and it is particularly acute in the state of Hawaii. An urgent need exists for the development of diagnostic imaging methods capable of revealing the associated biological processes involved at the molecular and cellular levels of blood vessels. Targeted ultrasound contrast agents add molecular imaging capabilities to diagnostic ultrasound. The unique advantages of ultrasound in terms of robustness, portability and relative low cost are thus incorporated into techniques to foster rapid translation. Ultrasound contrast agents are encapsulated micro-bubbles which oscillate nonlinearly about their equilibrium radius when acoustically excited. The agents produce unique scattering signatures which foster novel imaging methods that allow for the scattering signatures of blood to be distinguished from that of the surrounding tissue for freely circulating agents. A recent development has been contrast agents, which have specific ligands attached on their shell allowing binding at specific targeted sites. Though still in the early stages of development, targeted ultrasound contrast agents offer great potential for improved molecular imaging capabilities for diagnostic ultrasound. The specific aims of this study are to: 1) experimentally and theoretically characterize individual and groups of targeted agents near vascular walls and under physiological flow conditions from their scattering signatures for high frequency ultrasound and develop robust detection methods; and 2) investigate methods for image-guided assessment of leukocyte dysfunction for high frequencies. An understanding of how the close proximity of cell layers and likewise vessel walls change an ultrasound contract agents dynamics and scattering signature will provide the foundation for detection methods that can discriminate between bound and unbound agents.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 心血管疾病是美国的主要死因，在夏威夷州尤为严重。迫切需要开发能够揭示在血管的分子和细胞水平上涉及的相关生物过程的诊断成像方法。靶向超声造影剂增加了诊断超声的分子成像能力。因此，超声在鲁棒性、便携性和相对低成本方面的独特优势被纳入促进快速翻译的技术中。 超声造影剂是封装的微泡，当声激励时，微泡围绕其平衡半径非线性振荡。 所述试剂产生独特的散射特征，其促进新的成像方法，所述新的成像方法允许将血液的散射特征与周围组织的散射特征区分开以用于自由循环的试剂。 最近的发展是造影剂，其具有连接在其壳上的特异性配体，允许结合在特异性靶位点。尽管仍处于早期发展阶段，但靶向超声造影剂为改善超声诊断的分子成像能力提供了巨大潜力。 本研究的具体目的是：1）从实验和理论上表征血管壁附近和生理流动条件下的单个和组靶向药物，从它们的高频超声散射特征，并开发稳健的检测方法; 2）研究高频白细胞功能障碍的图像引导评估方法。 了解细胞层和血管壁的紧密接近如何改变超声接触剂的动力学和散射签名将提供基础的检测方法，可以区分绑定和未绑定的代理。