Effect of Dietary Omega-3 Fatty Acid Supplement on Prostate Cancer Metastasis
膳食 Omega-3 脂肪酸补充剂对前列腺癌转移的影响
基本信息
- 批准号:7176369
- 负责人:
- 金额:$ 14.78万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-05-01 至 2009-04-30
- 项目状态:已结题
- 来源:
- 关键词:Animal ModelAnimalsBiological AssayCancer PatientCarcinomaCarcinoma in SituClinicalConsumptionDataDevelopmentDietEatingFatty AcidsFemurFishesFollow-Up StudiesHealth ProfessionalInguinal lymph node groupInvasiveKnock-outKnockout MiceKnowledgeLacZ GenesLifeLungMalignant NeoplasmsMalignant neoplasm of prostateMetastatic Prostate CancerModelingMolecularMonitorMorbidity - disease rateNeoplasm MetastasisOmega-3 Fatty AcidsOrganPrimary NeoplasmProcessProspective StudiesProstateProstatic Intraepithelial NeoplasiasProstatic NeoplasmsProteinsRelative RisksResearch PersonnelRiskSiteSourceTimeTransgenesTumor BurdenWeekbonefeedinglymph nodesmenmortalitymouse modelneoplastic cellprogramstibiatumortumorigenesis
项目摘要
Metastasis, especially to the bone, is the most significant cause of morbidity and mortality in prostate cancer
patients. Therefore, development of relevant metastasis models for prostate cancer is of critical importance
for delineating the molecular mechanism underlining this process.
In our preliminary studies, we showed that omega-3 (n-3) polyunstatured fatty acids (PUFAs) can inhibit the
progression of prostate cancer in a Pten knockout mouse model, and increase survival of tumor-bearing
animals. However, the effect of n-3 PUFA on prostate cancer metastasis was not assessed. Epidemiological
data suggest that higher consumption of fish, a rich source of n-3 PUFA, is strongly associated with a
reduced risk of metastatic prostate cancer.
In this proposal, we will develop an animal model for spontaneous prostate cancer metastasis, and use this
model to determine the potential effect of n-3 PUFA on metastasis.
Aim 1. Develop a quantitative, live-observable model of prostate cancer metastasis. Enhanced yellow
fluorescent protein (EYFP) and lacZ markers will be used for live-monitoring and quantification of tumor
burden, respectively, at the primary tumor site and at the site of metastases. The transgenes will be
introduced into prostate-specific Pten knockout mice that mimic well the clinical course of human prostate
cancer, from prostatic intraepithelial neoplasia, carcinoma in situ, invasive carcinoma to metastasis. The
EYFP and LacZ markers will be expressed only in prostate tumor cells. Importantly, their levels of expression
will not be reduced or extinguished overtime or when prostate tumor cells metastasize to other organs such
as the bone, lung, and lymph nodes.
Aim 2. Determine the effect of dietary n-3 fatty acid supplement on prostate cancer metastasis.
Animals will be fed with diets containing various amounts of n-3 PUFA. Development of cancer in the
prostate and metastasis in the lung, inguinal lymph nodes and tibia/femur will be monitored. Tumor burden
will be quantified.
转移,尤其是转移到骨,是前列腺癌发病率和死亡率的最重要原因。
病人。因此,前列腺癌相关转移模型的建立具有重要意义。
来描述这一过程的分子机制。
在我们的初步研究中,我们发现omega-3(n-3)多不饱和脂肪酸(PUFAs)可以抑制
前列腺癌在Pten基因敲除小鼠模型中的进展和提高荷瘤小鼠的存活率
动物。然而,n-3多不饱和脂肪酸在前列腺癌转移中的作用尚未得到评估。流行病学
数据表明,更高的鱼类消费量,n-3多不饱和脂肪酸的丰富来源,与
降低转移性前列腺癌的风险。
在这项提案中,我们将建立一种前列腺癌自发转移的动物模型,并使用
模型以确定n-3多不饱和脂肪酸在转移中的潜在作用。
目的1.建立前列腺癌转移的定量、活体观察模型。增强型黄色
荧光蛋白(EYFP)和LacZ标记物将用于肿瘤的实时监测和量化
负荷分别位于原发肿瘤部位和转移部位。转基因将是
引入前列腺特异性Pten基因敲除小鼠,很好地模拟了人类前列腺的临床过程
癌症,从前列腺上皮内瘤变、原位癌、浸润性癌到转移。这个
EYFP和LacZ标记物将只在前列腺肿瘤细胞中表达。重要的是,他们的表达水平
不会随着时间的推移而减少或熄灭,或者当前列腺肿瘤细胞转移到其他器官时
如骨、肺和淋巴结节。
目的2.探讨饮食中补充n-3脂肪酸对前列腺癌转移的影响。
动物将被喂以含有不同数量的n-3多不饱和脂肪酸的饲料。癌症在中国的发展
将监测前列腺癌和肺、腹股沟淋巴结和胫骨/股骨的转移。肿瘤负担
将会被量化。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YONG Q CHEN其他文献
YONG Q CHEN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YONG Q CHEN', 18)}}的其他基金
Omega-3 PUFA-gene interaction in prostate cancer
Omega-3 PUFA 与前列腺癌中基因的相互作用
- 批准号:
8215565 - 财政年份:2012
- 资助金额:
$ 14.78万 - 项目类别:
Omega-3 PUFA-gene interaction in prostate cancer
Omega-3 PUFA 与前列腺癌中基因的相互作用
- 批准号:
8607164 - 财政年份:2012
- 资助金额:
$ 14.78万 - 项目类别:
Omega-3 PUFA-gene interaction in prostate cancer
Omega-3 PUFA 与前列腺癌中基因的相互作用
- 批准号:
8434839 - 财政年份:2012
- 资助金额:
$ 14.78万 - 项目类别:
Omega-3 PUFA-gene interaction in prostate cancer
Omega-3 PUFA 与前列腺癌中基因的相互作用
- 批准号:
8815275 - 财政年份:2012
- 资助金额:
$ 14.78万 - 项目类别:
North Carolina A&T University-Wake Forest Cancer Center partnership (2 of 2)
北卡罗来纳州A
- 批准号:
8135336 - 财政年份:2008
- 资助金额:
$ 14.78万 - 项目类别:
Effect of Dietary Omega-3 Fatty Acid Supplement on Prostate Cancer Metastasis
膳食 Omega-3 脂肪酸补充剂对前列腺癌转移的影响
- 批准号:
7393162 - 财政年份:2007
- 资助金额:
$ 14.78万 - 项目类别:
Mechanism of Fish Oil Supplements in Prevention of Cance
鱼油补充剂预防癌症的机制
- 批准号:
7136894 - 财政年份:2006
- 资助金额:
$ 14.78万 - 项目类别:
Anti-cancer mechanisms of n-3 PUFA mediated by syndecan
Syndecan介导的n-3 PUFA抗癌机制
- 批准号:
7152260 - 财政年份:2006
- 资助金额:
$ 14.78万 - 项目类别:
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 14.78万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 14.78万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 14.78万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 14.78万 - 项目类别:
Discovery Early Career Researcher Award
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Continuing Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
Analysis of thermoregulatory mechanisms by the CNS using model animals of female-dominant infectious hypothermia
使用雌性传染性低体温模型动物分析中枢神经系统的体温调节机制
- 批准号:
23KK0126 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Fund for the Promotion of Joint International Research (International Collaborative Research)
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 14.78万 - 项目类别:
Training Grant