Lipidomic Core

该实验室的主要功能是为研究人员提供质谱分析 参与该计划项目。具体而言，该核心将对以下内容进行定性和定量分析： 磷脂亚类和这些亚类的分子种类 串联质谱（ESI/MS/MS）。此外，该设施将量化5-HETE，PGE 2和其他 由组织培养样品产生的氧化多不饱和脂肪酸。样本的来源将是 从组织培养物和从动物模型收集的组织中。的灵敏度和动态范围 方法学可以测量从pmole到fmole的脂质量。根据要求，核心小组将执行并 开发先进的方法，以促进该计划项目的脂质分析。

The principle function of this laboratory is to provide mass spectrometry analysis for the investigators participating in the program project. Specifically this Core will perform qualitative and quantitative analysis of phospholipid subclasses and molecular species of these subclasses using state-of-the-art electrospray tandem mass spectrometry (ESI/MS/MS). In addition, the facility will quantify 5-HETE, PGE2, and other oxidized polyunsaturated fatty acids generated by tissue culture samples. The source of the samples will be from tissue culture and tissue collected from animal models. The sensitivity and dynamic range of the methodology can measure from pmole to fmole amounts of lipid. As required, the Core will implement and develop advanced methodologies to facilitate the analysis of lipids for the program project.