Thalamic constraints on cortical organization

丘脑对皮质组织的限制

• 批准号: 10735277
• 负责人: DARIO L RINGACH
• 金额: $ 59.44万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10735277
• 关键词: Address; Animals; Architecture; Cells; Central Nervous System Diseases; Classification; Cognition; Color; Cortical Column; Data; Development; Disease; Equilibrium; Etiology; Experimental Designs; Eye Movements; Frequencies; Goals; Human; Image; Individual; Knowledge; Link; Location; Locomotion; Mammals; Maps; Measures; Medical; Memory; Methods; Mission; Modeling; Mus; Neighborhoods; Neocortex; Neurons; Outcome; Perception; Process; Research; Retinal Ganglion Cells; Science; Signal Transduction; Site; Spatial Distribution; Stimulus; Structure; Surface; Techniques; Testing; Textbooks; Thalamic structure; United States National Institutes of Health; Vertebral column; Visual Fields; Visual System; Work; area striata; cortex mapping; experimental study; ganglion cell; imaging modality; innovation; neocortical; receptive field; response; scaffold; segregation; self organization; sex; two-photon; visual processing; visual stimulus

Project Summary / Abstract A long-term goal of our research is to understand how cortical neurons become tuned to stimulus features and organized into cortical maps. Our working hypothesis is that the spatial distribution of ON/OFF inputs from the geniculate seeds simple-cell receptive fields, cortical columns, and maps. This hypothesis stands in stark contrast with the textbook account that cortical receptive fields and maps develop through self- organizing processes driven by structured, spontaneous activity in the LGN. To test our hypothesis, we seek to investigate predicted correlations between the organization of the thalamocortical projection and the receptive fields and tuning of cortical neurons. In Aim 1, we seek to determine if there is a link between the spatial layout of ON/OFF domains, receptive field structure, and tuning in primary visual cortex. In Aim 2 we will classify and cluster thalamocortical boutons according to their responses to a rich set of visual stimuli, allowing us to reconstruct the tiling of the visual field by different classes of geniculate neurons. We will then study the spatial distribution and clustering of geniculate afferents into the cortex. Finally, in Aim 3, we will investigate a predicted correlation between the organization of thalamocortical projections and cortical organization in single animals. We will achieve this by simultaneously imaging the activity of thalamic boutons and cortical neurons using dual-color imaging. The proposed studies are significant as they test the possibility that an imbalance between the ON and OFF responses from the periphery provides a scaffold for the development of simple cell receptive fields and cortical columns. A positive outcome would prompt a revision of our current views of the development of the cortical architecture.

项目概要/摘要 我们研究的长期目标是了解皮层神经元如何适应刺激特征 并组织成皮质地图。我们的工作假设是开/关输入的空间分布 来自膝状种子的单细胞感受野、皮质柱和图。这个假设成立 与教科书上的说法形成鲜明对比的是，皮质感受野和地图是通过自我发展而发展的。 由 LGN 中结构化、自发的活动驱动的组织流程。为了检验我们的假设，我们 试图研究丘脑皮质投射的组织与 皮质神经元的感受野和调节。在目标 1 中，我们试图确定之间是否存在联系 开/关域的空间布局、感受野结构以及初级视觉皮层的调节。瞄准 2 我们将根据丘脑皮质按钮对丰富视觉的反应进行分类和聚类 刺激，使我们能够通过不同类别的膝状神经元重建视野的平铺。我们 然后将研究进入皮质的膝状传入神经的空间分布和聚集。最后，在目标 3，我们将研究丘脑皮质投射的组织与 单个动物的皮质组织。我们将通过同时对丘脑活动进行成像来实现这一目标 使用双色成像观察按钮和皮质神经元。拟议的研究意义重大，因为它们测试了 来自外围的 ON 和 OFF 响应之间的不平衡提供了一个支架 用于开发简单的细胞感受野和皮质柱。积极的结果将促使 对我们目前对皮质结构发展的看法的修订。