Gene expression and functional evolution in the Drosophila female reproductive tract
果蝇雌性生殖道的基因表达和功能进化
基本信息
- 批准号:10734757
- 负责人:
- 金额:$ 6.95万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:AffectAllelesBehaviorBiologyBirthCandidate Disease GeneCellsCodeCommunitiesComplementComplexConflict (Psychology)DataDefectDrosophila genusDrosophila melanogasterEjaculationEvolutionExclusionExhibitsExposure toFemaleFertilityGene ExpressionGenesGeneticGlandGoalsInvestigationInvestmentsKnock-outLabelLengthMeasuresMediatingMorphologyOrganOutcomePartner in relationshipPlayPopulationProcessProcessed GenesProteinsPublishingReceptor GeneReproductionReproductive BiologyResearchResolutionResourcesRoleSeminalSeminal fluidSiteTechnologyTestingTestisTimeTissuesTranscriptUnited States National Institutes of HealthUterusWorkcell typeexpectationflygene discoveryinsightinterestmalemodel organismmutantneuralnovelparalogous genepressurereceptorreproductivereproductive functionreproductive tractresponsesexsingle cell sequencingsingle-cell RNA sequencingsperm celltranscriptometranscriptome sequencing
项目摘要
PROJECT SUMMARY
The Drosophila female reproductive tract is morphologically and functionally complex, and as the arena where
high-stakes interactions among multiple male ejaculates and female products play out, it is also likely subject
to strong and conflicting selection pressures. Moreover, the reproductive tract is the initiation site for a cascade
of post-mating responses in female biology, from sequestering sperm and ovipositing to changes in neural
gene expression and behavior. Many of these processes may undergo sexually antagonistic selection, as
outcomes affect the sexes’ divergent reproductive interests. Nevertheless, research investment in the lower
female reproductive tract, including the sperm storage organs and the female accessory glands, severely lags
investment in male reproductive tissues. For example, the seminal receptacle, uterus, and female accessory
glands have been excluded from every major community transcriptome analysis; resources like Gal-4 drivers
or marker gene labels generally do not exist for these tissues; and the function and evolution of several organs
remain poorly described. These omissions limit insight into a range of important questions, including the
mechanisms and evolution of female-ejaculate interactions, the extent of rapid evolution in reproductive tissues
and its drivers, and the outcomes of sexual conflict. Yet these omissions also highlight an opportunity for new
low-barrier, high return investigations using well-established technologies in a leading model organism, which
this proposal pursues. First, single-cell sequencing of mated and unmated females from two diverged natural
populations will be used to 1) characterize cell-type diversity in the female reproductive tract for the first time
and 2) measure gene expression and divergence in the 5 somatic female reproductive tissues at cellular
resolution (Specific Aim 1). These data will also test the hypothesis that gene expression in female
reproductive tissues diverges rapidly owing to coevolving interactions with seminal fluid proteins. Next, genes
that are candidates for important functional evolution will be knocked out and evaluated for reproductive
effects. Specific Aim 2 tests null alleles of 7 receptors that are expressed in the seminal receptacle and show
indications of rapid divergence, to test the hypothesis that these receptors mediate female post-mating
responses. Specific Aim 3 uses a set of de novo genes with novel expression in the seminal receptacle to
explore the mysterious process of gene birth and functional integration. Specifically, the de novo gene
candidates that appear most likely to be functionally integrated based on transcript length, expression level,
structural complexity, and other metrics, will be knocked out and evaluated for effects on female reproductive
function.
项目摘要
果蝇雌性生殖道在形态上和功能上都很复杂,作为果蝇进行生殖活动的竞技场,
多个男性射精和女性产品之间的高风险相互作用发挥出来,它也可能是主题
强大而矛盾的选择压力。此外,生殖道是级联反应的起始部位,
交配后女性生物学反应,从隔离精子和产卵到神经系统的变化,
基因表达和行为。这些过程中的许多过程可能经历性拮抗选择,
结果影响到两性不同的生殖利益。尽管如此,研究投资在较低的
雌性生殖道,包括精子储存器官和雌性附腺,严重滞后
对雄性生殖组织的投资。例如,贮精器、子宫和女性附件
腺体已被排除在每一个主要的社区转录组分析;资源,如Gal-4驱动程序
或标记基因标签一般不存在这些组织;和几个器官的功能和进化
仍然描述得很差。这些遗漏限制了对一系列重要问题的深入了解,包括
雌性与射精相互作用的机制和进化,生殖组织快速进化的程度
及其驱动因素,以及性冲突的结果。然而,这些遗漏也凸显了新的机会,
在领先的模式生物中使用成熟的技术进行低障碍、高回报的研究,
这一建议继续下去。首先,对来自两个不同的自然群体的交配和未交配的雌性进行单细胞测序,
种群将被用于1)首次表征女性生殖道中的细胞类型多样性
和2)在细胞水平上测量5个体细胞雌性生殖组织中的基因表达和差异。
具体目标(1)。这些数据也将检验女性中基因表达的假设,
生殖组织由于与精液蛋白的共同进化相互作用而迅速分化。接下来是基因
重要功能进化的候选者将被淘汰并进行生殖评估
方面的影响.特异性目的2检测在贮精器中表达的7种受体的无效等位基因,并显示
快速分化的迹象,以检验这些受体介导雌性交配后的假设
应答Specific Aim 3使用一组在贮精器中具有新表达的从头基因,
探索基因诞生和功能整合的神秘过程。具体来说,
基于转录本长度,表达水平,
结构复杂性和其他指标,将被淘汰,并评估对女性生殖的影响,
功能
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Rachel C Thayer其他文献
Rachel C Thayer的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似海外基金
Linkage of HIV amino acid variants to protective host alleles at CHD1L and HLA class I loci in an African population
非洲人群中 HIV 氨基酸变异与 CHD1L 和 HLA I 类基因座的保护性宿主等位基因的关联
- 批准号:
502556 - 财政年份:2024
- 资助金额:
$ 6.95万 - 项目类别:
Olfactory Epithelium Responses to Human APOE Alleles
嗅觉上皮对人类 APOE 等位基因的反应
- 批准号:
10659303 - 财政年份:2023
- 资助金额:
$ 6.95万 - 项目类别:
Deeply analyzing MHC class I-restricted peptide presentation mechanistics across alleles, pathways, and disease coupled with TCR discovery/characterization
深入分析跨等位基因、通路和疾病的 MHC I 类限制性肽呈递机制以及 TCR 发现/表征
- 批准号:
10674405 - 财政年份:2023
- 资助金额:
$ 6.95万 - 项目类别:
An off-the-shelf tumor cell vaccine with HLA-matching alleles for the personalized treatment of advanced solid tumors
具有 HLA 匹配等位基因的现成肿瘤细胞疫苗,用于晚期实体瘤的个性化治疗
- 批准号:
10758772 - 财政年份:2023
- 资助金额:
$ 6.95万 - 项目类别:
Identifying genetic variants that modify the effect size of ApoE alleles on late-onset Alzheimer's disease risk
识别改变 ApoE 等位基因对迟发性阿尔茨海默病风险影响大小的遗传变异
- 批准号:
10676499 - 财政年份:2023
- 资助金额:
$ 6.95万 - 项目类别:
New statistical approaches to mapping the functional impact of HLA alleles in multimodal complex disease datasets
绘制多模式复杂疾病数据集中 HLA 等位基因功能影响的新统计方法
- 批准号:
2748611 - 财政年份:2022
- 资助金额:
$ 6.95万 - 项目类别:
Studentship
Genome and epigenome editing of induced pluripotent stem cells for investigating osteoarthritis risk alleles
诱导多能干细胞的基因组和表观基因组编辑用于研究骨关节炎风险等位基因
- 批准号:
10532032 - 财政年份:2022
- 资助金额:
$ 6.95万 - 项目类别:
Recessive lethal alleles linked to seed abortion and their effect on fruit development in blueberries
与种子败育相关的隐性致死等位基因及其对蓝莓果实发育的影响
- 批准号:
22K05630 - 财政年份:2022
- 资助金额:
$ 6.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigating the Effect of APOE Alleles on Neuro-Immunity of Human Brain Borders in Normal Aging and Alzheimer's Disease Using Single-Cell Multi-Omics and In Vitro Organoids
使用单细胞多组学和体外类器官研究 APOE 等位基因对正常衰老和阿尔茨海默病中人脑边界神经免疫的影响
- 批准号:
10525070 - 财政年份:2022
- 资助金额:
$ 6.95万 - 项目类别:
Leveraging the Evolutionary History to Improve Identification of Trait-Associated Alleles and Risk Stratification Models in Native Hawaiians
利用进化历史来改进夏威夷原住民性状相关等位基因的识别和风险分层模型
- 批准号:
10689017 - 财政年份:2022
- 资助金额:
$ 6.95万 - 项目类别: