Markers of sensivity to the EGFR inhibitor erlotinib in breast cancer

描述（由申请人提供）：表皮生长因子受体（EGFR）信号通路是细胞生长和增殖的中心调节剂，并调节关键的细胞周期调节分子。这种途径已经成为癌症治疗的一个有希望的靶点。EGFR酪氨酸激酶抑制剂（TKIs），如埃洛替尼（特罗凯）和吉非替尼（易瑞沙），已被批准用于癌症治疗，但仅在一个亚组患者中引起临床反应。因此，EGFR-TKI的分子作用机制还有待进一步研究。我的长期目标是阐明EGFR-TKI的分子作用机制，从而开发出与临床相关的新型治疗方法或诊断工具。我最近表明，体外埃洛替尼敏感性部分依赖于周期蛋白依赖性激酶2 （Cdk2）的活性，这是EGFR途径中最下游的激酶，调节从G1期到S期的转变。本应用的目的是确定EGFR和非EGFR信号通路的哪些下游分子预测了对EGFR- tkis的反应。该提议的中心假设是Cdk2活性对egfr - tki介导的细胞毒性的影响是由细胞信号通路的下游分子，特别是ERK， p27和PEA15调节的。这一假设是基于以下观察。首先，厄洛替尼抑制厄洛替尼敏感和厄洛替尼耐药乳腺癌细胞中EGFR的酪氨酸激酶；然而，厄洛替尼仅在敏感细胞中抑制Cdk2活性。这些发现表明，在厄洛替尼耐药细胞中存在EGFR信号通路异常。其次，在厄洛替尼敏感细胞中，磷酸化的ERK下调，p27上调，p27的磷酸化状态影响其核胞质定位和表达水平。第三，PEA15将ERK从细胞核隔离到细胞质中，减少细胞增殖。我设计了三个独立但相互关联的特定目标，以提供对经EGFR- tki治疗的乳腺癌细胞中下游EGFR和非EGFR信号通路的全面评估。具体目标建立厄洛替尼如何调节p27抑制Cdk2活性的体外实验。具体目标2。建立PEA15在体外和体内如何调节厄洛替尼的敏感性。具体目标3。建立预测厄洛替尼敏感性的体内生物标志物。相关性：本研究将为了解受EGFR-TKI影响的下游分子的作用提供重要见解。我们的研究结果将通过提高EGFR-TKI的疗效和促进选择可能受益于EGFR-TKI治疗的患者来改善癌症患者的预后。

DESCRIPTION (provided by applicant): The epidermal growth factor receptor (EGFR) signaling pathway is a central regulator of cell growth and proliferation and modulates critical cell cycle regulatory molecules. This pathway has emerged as a promising target for cancer therapy. EGFR tyrosine kinase inhibitors (TKIs), such as erlotinib (Tarceva) and gefitinib (Iressa), are approved for cancer treatment but have induced a clinical response in only a subgroup of patients. Therefore, EGFR-TKI's molecular mechanism of action needs to be better understood. My long-term goal is to elucidate the molecular mechanism of action of EGFR-TKI so that novel therapeutic approaches or diagnostic tools that are clinically relevant can be developed. I have recently shown that in vitro erlotinib sensitivity is partially dependent on the activity of cyclin-dependent kinase 2 (Cdk2), which is the most downstream kinase of the EGFR pathway that regulates the transition from the G1 phase to the S phase. The objective of this application is to determine which downstream molecules of the EGFR and non-EGFR signaling pathway predict the response to EGFR-TKIs. The central hypothesis of this proposal is that the effect of Cdk2 activity on EGFR-TKI-mediated cytotoxicity is regulated by downstream molecules of the cell signaling pathway, specifically ERK, p27, and PEA15. This hypothesis is based on the following observations. First, erlotinib inhibits the tyrosine kinase of EGFR in both erlotinib-sensitive and erlotinib-resistant breast cancer cells; however, erlotinib inhibits Cdk2 activity only in sensitive cells. These findings indicate that there is an abnormality in the EGFR signaling pathway in erlotinib-resistant cells. Second, phosphorylated ERK is downregulated and p27 is upregulated in erlotinib-sensitive cells, and the phosphorylation status of p27 affects its nuclear-cytoplasmic localization and expression level. Third, PEA15 sequesters ERK into the cytoplasm from the nucleus and reduces cell proliferation. I have designed three independent but interrelated specific aims to provide a comprehensive assessment of the downstream EGFR and non-EGFR signaling pathway in breast cancer cells treated with EGFR-TKI. Specific Aim 1. Establish how erlotinib regulates p27 to suppress Cdk2 activity in vitro. Specific Aim 2. Establish how PEA15 modulates erlotinib sensitivity in vitro and in vivo. Specific Aim 3. Establish in vivo biomarkers that predict erlotinib sensitivity. Relevance: This study will provide significant insight into the role of downstream molecules affected by EGFR-TKI. Our findings will improve the outcome of cancer patients by increasing EGFR-TKI efficacy and facilitating the selection of patients who may benefit from EGFR-TKI therapy.