Inhibition of Carcinogenesis by Tea and Tea Constituents

茶和茶成分抑制致癌作用

• 批准号: 7367995
• 负责人: Zigang Dong
• 金额: $ 26.03万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7367995
• 关键词: Binding; Binding Proteins; Black Tea; Cancer Model; Carcinogenesis Inhibition; Chemoprevention; Chemopreventive Agent; Condition; Cultured Cells; Development; Duodenum; Epigallocatechin Gallate; Esophagus; Exhibits; Goals; Growth Factor; Insulin; Insulin-Like Growth Factor I; Investigation; Knowledge; Lead; Liver; Lung; Malignant Neoplasms; Mammary gland; Modeling; Molecular; Molecular Target; Neoplastic Cell Transformation; Pancreas; Play; Preparation; Protein Analysis; Proteins; Rodent Model; Role; Skin Cancer; Small Intestines; Stomach; Tea; Testing; Tumor Promoters; Tumor Promotion; Tumorigenicity; Vimentin; Xenograft Model; base; cancer chemoprevention; carcinogenesis; cell growth; cell transformation; design; gallocatechol; in vivo; mouse model; novel; polyphenol; receptor; tumor; tumor xenograft; tumorigenesis

This is a new R01 application to investigate the molecular targets of the chemopreventive effect on cancer by tea polyphenols. Recently, we have identified a few polyphenol (-)epigallocatechin 3-gallate (EGCG) binding proteins. Because these EGCG-binding proteins are critical for cell growth, neoplastic cell transformation and tumorigenicity, we hypothesize that the chemopreventive effect of the tea polyphenol EGCG and its derivatives is derived from their target on these proteins. We will use 3H-EGCG cell culture and protein analysis to examine the anti-tumor promotion activity of tea polyphenols. The specific aiss to addrress the hypothesis are 1) to study the role of newly identified EGCG-binding proteins in tumor promoter-induced cell growth and cell transformation; 2) to study the role of EGCG-binding proteins in chemopreventive activity of EGCG and other tea polyphenols by using cell culture models; 3) to study the interactions of EGCG with its binding protein fyn and IGF-1R; 4) to study the role of fyn and IGF-1R as molecular targets fo rthe anti-tumor effect of EGCG in vivo in tumor xenograft model. We have established experimental conditions through preliminary investigations and expect that the proposed molecular mechanism studies will lead to the development of better chemopreventive agents and facilitate the design of more effective chemoprevention trials.

这是一种新的R01应用，通过以下方式研究癌症化学预防作用的分子靶点 茶多酚。最近，我们发现了一些多酚(-)表没食子儿茶素没食子酸酯(EGCG)结合 蛋白质。因为这些EGCG结合蛋白对细胞生长、肿瘤细胞转化和 致瘤性，我们假设茶多酚EGCG及其化合物的化学预防作用 衍生物是从它们在这些蛋白质上的靶标衍生出来的。我们将使用~H-EGCG细胞培养和蛋白质 分析检测茶多酚的抗肿瘤促进活性。地址的具体地址 假设1)研究新发现的EGCG结合蛋白在肿瘤启动子诱导的细胞中的作用 2)研究EGCG结合蛋白在细胞化学预防中的作用。 利用细胞培养模型研究EGCG与茶多酚的相互作用 结合蛋白Fyn和IGF-1R；4)研究Fyn和IGF-1R作为抗肿瘤分子靶点的作用 EGCG在异种移植瘤模型中的体内作用我们已经建立了实验条件 初步调查，并预期拟议的分子机制研究将导致 开发更好的化学预防药物，促进设计更有效的化学预防 审判。