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Molecular Mechanisms and Targets of Soy Compounds in the Prevention of Colon Canc

大豆化合物预防结肠癌的分子机制和靶点

基本信息

批准号：
8422792
负责人：
Zigang Dong
金额：
$ 16.58万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-01-01 至 2014-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8422792
关键词：
Asia Binding Biological Assay Bladder Breast Cancer Etiology Cell Culture Techniques Cessation of life Chemopreventive Agent Colon Colon Carcinoma Computer Simulation Computers Consumption Country Diet Docking Effectiveness Endometrial Epidemiologic Studies Esophageal Exhibits Food Genetic Goals Health Human Isoflavones Life Style Lung Malignant Neoplasms Methods Modeling Molecular Molecular Biology Molecular Target NIH Program Announcements Nutritional Obesity Observational Study Ovarian Pattern Physical activity Population Preclinical Drug Evaluation Prevention Prostate Protein Structure Databases Proteins Risk Safety Shapes Skin Stomach Technology Testing Thyroid Gland United States base bioactive food component cancer prevention cancer risk cancer therapy cancer type cellular targeting daidzein data management equol in vivo in vivo Model lifestyle factors models and simulation mouse model protein structure function public health relevance response screening soy soy protein isolate supercomputer

项目摘要

DESCRIPTION (provided by applicant): This R21 proposal is submitted in response to Program Announcement Number PA-10-088 entitled "Exploratory Cancer Prevention Studies Involving Molecular Targets for Bioactive Food Components." Consumption of soy based foods has been shown to exhibit varying degrees of inhibitory effects on numerous types of cancers. Colon cancer is a common cause of cancer death in the United States. Diet and lifestyle factors, including obesity and lack of physical activity, are believed to be associated with increased colon cancer risk. Numerous observational studies in humans have indicated that a high consumption of isoflavones or soy in certain countries is associated with a reduced risk of colon cancer. However, the evidence for positive health effects of the isoflavones in soy is quite inconsistent. Rigorous mechanistic studies are needed to elucidate the cellular targets and mechanisms of action of soy compounds against colon and other cancers. We propose to combine analyses of protein structure and function with advanced methods of data management and drug screening to identify potential target proteins for three isoflavone soy compounds. We will use supercomputer technology combined with molecular biology, cell culture models and an in vivo mouse model to test and validate the computer prediction of the molecular target(s) of daidzein and three of its metabolites.

描述（由申请人提供）：本 R21 提案是为了响应编号为 PA-10-088 的项目公告而提交，题为“涉及生物活性食品成分的分子靶标的探索性癌症预防研究”。食用大豆食品已被证明对多种癌症具有不同程度的抑制作用。结肠癌是美国癌症死亡的常见原因。饮食和生活方式因素，包括肥胖和缺乏体力活动，被认为与结肠癌风险增加有关。大量人类观察研究表明，在某些国家，大量食用异黄酮或大豆与降低结肠癌风险有关。然而，关于大豆中异黄酮对健康产生积极影响的证据却相当不一致。需要进行严格的机制研究来阐明大豆化合物对抗结肠癌和其他癌症的细胞靶点和作用机制。我们建议将蛋白质结构和功能分析与先进的数据管理和药物筛选方法相结合，以确定三种异黄酮大豆化合物的潜在靶蛋白。我们将利用超级计算机技术结合分子生物学、细胞培养模型和体内小鼠模型来测试和验证计算机对大豆苷元及其三种代谢物分子靶点的预测。