The Psp response of Yersinia enterocolitica

小肠结肠炎耶尔森氏菌的 Psp 反应

• 批准号: 7186637
• 负责人: ANDREW J. DARWIN
• 金额: $ 32.05万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-15 至 2008-07-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7186637
• 关键词: Animals; Awareness; Bacteria; Bacteriophages; Binding Sites; Biochemical Genetics; Bioterrorism; Cell membrane; Class; Condition; Cytoplasmic Protein; DNase-I Footprinting; Data; Defect; Disease; Enterobacteriaceae; Escherichia coli; Gastrointestinal Diseases; Genes; Genome; Growth; In Vitro; Infection; Investigation; Location; Modeling; Molecular; Mutagenesis; Mutation; Operon; Organism; Pasteurella pseudotuberculosis; Pathogenesis; Physiological; Plague; Play; Production; Protein Family; Proteins; Regulon; Research Personnel; Role; Secretin; Shock; Signal Transduction; Stimulus; Stress; Symptoms; System; Testing; Transcription Coactivator; Type III Secretion System Pathway; Vibrio cholerae; Virulence; Virulence Factors; Yersinia; Yersinia enterocolitica; Yersinia pestis; biological adaptation to stress; cell envelope; env Gene Products; human disease; in vivo; insight; intracellular protein transport; mouse model; mutant; pathogen; programs; protein localization location; response

DESCRIPTION (provided by applicant): Bacteria of the genus Yersinia are responsible for a variety of human diseases. Y. pestis causes Bubonic Plague, and has recently regained prominence in public awareness due to its potential use as an agent of bioterrorism. In contrast, Y. pseudotuberculosis and Y. enterocolitica cause primarily gastrointestinal disease. However, despite the differences in disease symptoms, the three pathogenic Yersinia species are closely related, and share several common virulence determinants. Yersinia studies have provided fundamental insights into bacterial pathogenesis, including the first example of the widespread type III secretion system (TTSS). In Yersinia, as in all bacterial pathogens, many of the proteins that play important roles in virulence, including components of the TTSS, are located in the cell envelope. Under certain conditions, some envelope proteins become misfolded/mislocalized. Specific stress-response mechanisms deal with this problem, examples of which are the RpoE and Cpx systems of Escherichia coli and related organisms. These extracytoplasmic stress responses play important roles during host infection. The central hypothesis of this proposal is that a different extracytoplasmic stress response system is encoded by the phage-shock-protein locus (psp) of Y. enterocolitica. A Y. enterocolitica psp mutant is avirulent, and homologus psp loci are found in other bacterial pathogens, including Y. pestis and Vibrio cholerae. Our preliminary data indicate that the Psp system responds to mislocalization of several envelope proteins involved in virulence, including at least one component of a TTSS. By studying the Psp system we will gain further insight into the essential ability of bacteria to respond to stressful conditions that occur during host infection. Specifically, we propose to: (1) Analyze the proteins that induce the Psp system and characterize any overlap between Psp inducers and RpoE/Cpx inducers; (2) Determine the topology of the Psp system, and investigate how Psp allows extracytoplasmic stress to be sensed and signaled across the cytoplasmic membrane; (3) Characterize genes directly controlled by the Psp system, in order to identify further stress response components.

描述（由申请人提供）：Yersinia属的细菌负责各种人类疾病。 Y. Pestis引起了鼠疫，并且由于其潜在用作生物恐怖主义的代理人，最近重新获得了公众意识的突出。相比之下，Y. pseudotuberculosis和Y.肠结肠炎主要引起胃肠道疾病。然而，尽管疾病症状存在差异，但三种致病性耶尔森氏菌种类密切相关，并具有几种常见的毒力决定因素。 Yersinia的研究提供了对细菌发病机理的基本见解，包括广泛的III型分泌系统（TTSS）的第一个例子。在耶尔森氏菌中，与所有细菌病原体一样，许多在毒力中起重要作用的蛋白质，包括TTSS的成分，都位于细胞包膜中。在某些条件下，某些包膜蛋白被错误折叠/错误定位。特定的应激响应机制涉及此问题，其中示例是大肠杆菌和相关生物的RPOE和CPX系统。这些外质应力反应在宿主感染过程中起着重要作用。该提案的中心假设是，Y. enterocolitica的噬菌体 - 蛋白基因座（PSP）编码了不同的外胞质应力反应系统。 Y. Enterocolitica PSP突变体是无毒的，并且在其他细菌病原体（包括Y. Pestis和Vibrio Cholerae）中发现了同型PSP基因座。我们的初步数据表明，PSP系统响应了涉及毒力的几种包膜蛋白的错误定位，包括TTSS的至少一个成分。通过研究PSP系统，我们将进一步了解细菌应对宿主感染过程中发生的压力状况的基本能力。具体而言，我们建议：（1）分析诱导PSP系统的蛋白质并表征PSP诱导剂与RPOE/CPX诱导剂之间的任何重叠； （2）确定PSP系统的拓扑，并研究PSP如何在细胞质膜上感测并发出信号； （3）表征由PSP系统直接控制的基因，以识别进一步的应力响应成分。