Implantable Microarray Probe for Real-Time Glutamate and GABA Detection

用于实时谷氨酸和 GABA 检测的植入式微阵列探针

基本信息

  • 批准号:
    10761486
  • 负责人:
  • 金额:
    $ 90.4万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-09-18 至 2025-08-31
  • 项目状态:
    未结题

项目摘要

Project Summary This STTR proposal will focus on developing and testing a novel, first-on-the-market implantable biosensor for in vivo, real-time sensing of gamma-aminobutyric acid (GABA) and glutamate (GLU) for animal studies. GABA and GLU are neurotransmitters (NTs) that are essential for information processing and plasticity, memory, and other functions. GLU is the major excitatory NT and GABA is the major inhibitory NT; a proper balance between them is vital for normal brain function. GLU-GABA dysregulation plays a critical role in several brain disorders, including epilepsy (a disease affecting 1.2% of Americans), dementia (which will affect 130M worldwide by 2050) and Parkinson’s (which affects 1.5M Americans today). A fundamental understanding of NT homeostasis including its temporal components and its role on behavioral events within and across brain areas would lead to a better understanding of human brain function and to new and more effective treatments. Existing NT sensing methods measure only one NT at a time, suffer from poor spatiotemporal resolution, are unable to measure NT dynamics at the circuit level, continuously in real time. Our goal is to develop an ultra-small, flexible (50µm) neural probe for chronic, direct and simultaneous amperometric detection of GLU&GABA, with sub-second temporal resolution and with no externally applied reagents. Phase I focused on manufacturing a prototype Si penetrating shank-type probe with 4 micropatterned sensors and one microfluidic on-demand in-situ calibrator (ODIC), optimizing the enzyme functionalization process, and performing a feasibility study on the measurement of physiologically-relevant changes in the levels of GLU&GABA in real time for freely moving rats for up to 2 weeks. The objectives of Phase II are an upgrade of the probe into a brain micromotion-resistant hybrid Si- flexible polymer probe of higher functionality (octrode + 2 ODIC micro-channels), improvements in selective functionalization, and validation of the probes in a rat model of temporal lobe epilepsy. The multifunctional ODICs will be applied to perform in-situ calibrations for chronic measurements and to investigate the circuit activity of 3 adjacent cortical layers in the whisker barrel cortex via chemical modulation. Upon completion, we expect to deliver a unique-on-the-market dual NT probe of excellent reliability and superior sensitivity, selectivity, and stability, with all performance parameters equal to or better than those offered by current technologies. To achieve this, Alcorix will partner with experts in amperometric NT sensing research from Louisiana Tech U. and experts in the manufacture of advanced neuroprobes from NeuroNexus, who will assist with hybrid Si-flexible polymer integration, in vitro and in vivo evaluation, and eventual market entry. The proposed research will also enable alternative uses such as point-of-use sensors for neurotoxins or disease bio-markers, and neural signal recording or neurostimulation in conjunction with specific locally-injected drugs. The 2023 world market for electrophysiological neuro-probes (Grand View Research) is about $266M, CAGR of 3.1%. If only 1% of that market can be captured by this technology it would be more than sufficient justification for the proposed work.
项目摘要 该STTR提案将专注于开发和测试一种新型的,市场上第一个可植入的生物传感器, 用于动物研究的γ-氨基丁酸(GABA)和谷氨酸(GLU)的体内实时感测。GABA 和GLU是神经递质(NT),对信息处理和可塑性、记忆和 其他功能。GLU是主要的兴奋性NT,GABA是主要的抑制性NT; 它们对正常的大脑功能至关重要。GLU-GABA失调在多种脑部疾病中发挥着关键作用, 包括癫痫(影响1.2%的美国人的疾病),痴呆症(到2050年将影响全球1.3亿人) 和帕金森氏症(今天影响着150万美国人)。对NT稳态的基本理解 包括它的时间成分和它对大脑区域内和跨大脑区域的行为事件的作用, 更好地了解人类大脑的功能,以及更有效的新治疗方法。现有NT传感 方法一次只能测量一个NT,时空分辨率差,不能测量NT 电路级的动态,连续的真实的时间。我们的目标是开发一种超小型、灵活的(150 µm) 神经探针,用于GLU和GABA的慢性、直接和同时安培检测,亚秒 时间分辨率和没有外部施加的试剂。第一阶段的重点是制造一个原型硅 具有4个微图案化传感器和一个微流体按需原位校准器的穿透柄型探针 (ODIC),优化酶功能化过程,并对测量进行可行性研究 自由活动大鼠在真实的时间内GLU和GABA水平的生理相关变化, 周第二阶段的目标是将探测器升级为大脑微动抵抗混合硅- 更高功能性的柔性聚合物探针(八极+ 2个ODIC微通道), 功能化和在颞叶癫痫大鼠模型中验证探针。多功能ODIC 将用于执行慢性测量的原位校准,并调查3 通过化学调制在须桶皮质中的相邻皮质层。完成后,我们预计 提供市场上独一无二的双NT探头,具有出色的可靠性和上级灵敏度、选择性, 稳定性,所有性能参数等于或优于当前技术提供的性能参数。到 为了实现这一目标,Alcorix将与路易斯安那理工大学的电流NT传感研究专家合作。和 来自NeuroNexus的先进神经探针制造专家,他们将协助混合硅柔性 聚合物整合、体外和体内评估以及最终的市场进入。拟议的研究还将 实现替代用途,例如用于神经毒素或疾病生物标志物和神经信号的使用点传感器 记录或神经刺激结合特定的局部注射药物。2023年世界市场 电生理神经探针(Grand View Research)约为2.66亿美元,复合年增长率为3.1%。如果只有百分之一 市场可以捕捉到这种技术,这将是足够的理由,拟议的工作。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Prabhu Arumugam其他文献

Prabhu Arumugam的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

相似海外基金

RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Standard Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Standard Grant
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Training Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Studentship
ERI: Developing a Trust-supporting Design Framework with Affect for Human-AI Collaboration
ERI:开发一个支持信任的设计框架,影响人类与人工智能的协作
  • 批准号:
    2301846
  • 财政年份:
    2023
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Standard Grant
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Operating Grants
How motor impairments due to neurodegenerative diseases affect masticatory movements
神经退行性疾病引起的运动障碍如何影响咀嚼运动
  • 批准号:
    23K16076
  • 财政年份:
    2023
  • 资助金额:
    $ 90.4万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了