A quick, and robust, and unbiased platform for circular RNAs isolation, discovery and profiling.

一个快速、强大且公正的循环 RNA 分离、发现和分析平台。

• 批准号: 10761203
• 负责人: Prashant K. Khade
• 金额: $ 35.29万
• 依托单位: RIBO-THERAPEUTICS LLC
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-09 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10761203
• 关键词: Acceleration; Address; Affect; Benchmarking; Biochemical; Biochemistry; Bioinformatics; Biology; Biotinylation; Buffers; California; Cell physiology; Cells; Complex; Computational Biology; DNA; Data; Detection; Development; Digestion; Disease; Environment; Eukaryotic Cell; Exons; Gene Expression; Genomics; Goals; Hour; Human; Hydroxyl Radical; Immunity; Introns; Ligation; Malignant Neoplasms; Maps; Medical; Messenger RNA; Methodology; Methods; MicroRNAs; Modification; Natural Immunity; Nature; Pathologic; Pathway interactions; Phase; Physiological; Poly A; Poly(A) Tail; Positioning Attribute; Primary carcinoma of the liver cells; Principal Investigator; Process; Publications; Pulmonary Fibrosis; RNA; RNA Sequences; RNA Splicing; Regulator Genes; Reporting; Reproducibility; Role; Sampling; Sensitivity and Specificity; Specificity; Streptavidin; Structure; Technology; Tissues; Universities; Virus Diseases; bioinformatics pipeline; cell type; cellular pathology; circular RNA; comparative efficacy; comparison control; efficacy evaluation; improved; insight; magnetic beads; nervous system disorder; novel; nuclease; predictive tools; ribonuclease R; transcriptome; transcriptome sequencing

The goal of this proposal is to develop and validate a novel platform for the enrichment of the complete repertoire of circular RNAs (circRNAs). Thousands of circRNAs have been reported since their discovery >40 years ago from human transcriptome. The importance of circRNAs can be gauged from the fact that they are broadly expressed in eukaryotic cells, show cell and tissue-type specificity; and their expression is often conserved across species. CircRNAs have been implicated in neurological diseases, innate immunity, hepatocellular carcinoma and lung fibrosis demonstrating their role in cellular physiological and pathological pathways. The present approaches are reported to be biased in enriching circRNAs repertoire. Given their importance in various diseases, there is an urgent and unmet need for efficient circRNA isolation technology. In Phase-I application, we proposed two specific aims to develop Quick-circRNA platform, validate and compare its efficacy with current methods. In aim-1 we will develop and establish Quick-circRNA platform in various conditions and cell types in obtaining uniform an unbiased enrichment of circRNAs. In aim-2 we will compare the efficacy of Quick-circRNA with known methods in various cellular conditions. We believe that phase-I proposal will provide a benchmark for phase-II, which can streamline the Quick-circRNA platform for enriching circRNAs with high reproducibility and efficiency. In the end, we will develop a quick, and robust, and unbiased platform for circular RNAs isolation, discovery and profiling.

该提案的目标是开发和验证一个新的平台，以丰富完整的曲目 环状RNA（CircRNA）自40多年前发现以来，已有数千种circRNA被报道 从人类转录组中circRNA的重要性可以从它们广泛存在于 在真核细胞中表达，显示细胞和组织类型特异性;并且它们的表达通常是保守的 跨越物种。CircRNA与神经系统疾病、先天性免疫、肝细胞疾病、肝细胞癌和肝细胞癌有关。 癌和肺纤维化，表明它们在细胞生理和病理途径中的作用。的 据报道，目前的方法偏向于富集circRNA库。鉴于其在各种领域的重要性， 疾病，对有效的circRNA分离技术存在迫切且未满足的需求。在第一阶段应用中， 我们提出了两个具体目标，以开发Quick-circRNA平台，验证并比较其与当前 方法.在aim-1中，我们将在不同的条件和细胞类型下开发和建立Quick-circRNA平台， 获得均匀无偏的circRNA富集。在aim-2中，我们将比较Quick-circRNA 在各种细胞条件下使用已知方法。我们认为，第一阶段建议将提供一个基准 对于II期，它可以简化Quick-circRNA平台，以高重现性富集circRNA 效能最后，我们将开发一个快速，稳健，无偏见的环状RNA分离平台， 发现和分析