Automation of Liquid Nitrogen Freezer for Cryopreservation of Unique Human Biospecimens at the Vanderbilt Biospecimen Storage Facility

在范德比尔特生物样本储存设施中使用液氮冷冻机自动化冷冻保存独特的人类生物样本

• 批准号: 10736276
• 负责人: Alexander Bick
• 金额: $ 25.28万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10736276
• 关键词: Academic Medical Centers; Acceleration; Automation; Automobile Driving; Award; Basic Science; Biological; Biological Markers; Biological Sciences; COVID-19 pandemic; COVID-19 patient; COVID-19 prevention; Canada; Clinic; Clinical; Clinical Research; Clinical Sciences; Collection; Cooperative Human Tissue Network; Cryopreservation; Data; Disease; Disease Progression; Ensure; Environment; Friends; Funding; Future; Genetic; Genotype; Glass; Human; Individual; Infrastructure; Institution; International; Investigation; Investments; Link; Liquid substance; Monitor; Monoclonal Antibodies; National Cancer Institute; Nitrogen; Phenotype; Reagent; Recovery; Research Personnel; Resource Sharing; Resources; Retrieval; Risk; Robotics; SARS-CoV-2 antibody; Safety; Sampling; Services; Specimen; System; Technology; Temperature; Tissue Sample; Tissues; Transition Temperature; Translational Research; Translations; United States; United States National Institutes of Health; Vaccines; Water; Withdrawal; animal tissue; anticancer research; biobank; cancer care; cell preparation; cost; cost effective; cryogenics; disorder risk; high dimensionality; human disease; human tissue; improved; migration; personalized medicine; preservation; repository; treatment response; vaccinology

Project Summary Large, high-dimensional clinical, genetic and biomarker data linked to bio-repositories are driving basic, clinical and translational research. For the past 20 years, Vanderbilt University Medical Center (VUMC) has made long-term strategic investments in translational sciences as a platform for excellence across clinical and basic science departments and is internationally recognized as a leader in such fields as personalized medicine, vaccinology, and cancer care and research. As examples, BioVU, Vanderbilt’s large-scale biorepository, contains detailed de-identified human phenotype information linked to de-identified discarded samples and genotypes. Viably cryopreserved human specimens enabled the Vanderbilt Vaccine Center to isolate monoclonal antibodies against COVID-19 that were developed into Evusheld, a therapy to prevent COVID-19 in at-risk individuals. Cooperative Human Tissue Network at VUMC is one of only six funded by the National Cancer Institute to procure and distribute human tissues to researchers throughout the US, Canada, and internationally. These resources and others provide an accelerating engine of discovery. Collectively these endeavors are providing powerful predictors of disease risk, disease progression or response to therapy and underpinning studies delineating the fundamental mechanism of disease as well as translation to the clinic. These large-scale initiatives generate a massive number of valuable human and animal tissue samples. These tissues can only be viably cryopreserved when stored in liquid nitrogen. The size of these biorepositories is exceeding the capacity of individual investigators or groups to effectively curate, maintain sample integrity and rapidly retrieve samples for further study. Many of these materials could not be replaced and are of international importance. To meet this large and growing need, a specialized automated -190°C Cryogenic Freezer for Sample Storage system is necessary to manage, store and retrieve the tens of thousands of biorepository samples. This proposal seeks to obtain funding for the purchase of an Azenta BioStore Cryo M60 robotic liquid nitrogen freezer system that can accommodate all large biorepositories at Vanderbilt. With such a system Vanderbilt can provide the necessary institutional support, environment and expertise to ensure cost- effective, quality assured management, storage and retrieval of these critical samples and reagents. The technology is durable and scalable and therefore it can be predicted that this automated system will enable a broad range of investigators and propel basic, clinical and translational research long into the future.

项目概要 与生物存储库相关的大型、高维临床、遗传和生物标志物数据正在推动基础、临床 和转化研究。过去 20 年来，范德比尔特大学医学中心 (VUMC) 取得了 对转化科学的长期战略投资，作为跨临床和基础的卓越平台 科学部门，是国际公认的个性化医疗等领域的领导者， 疫苗学、癌症护理和研究。例如，范德比尔特大学的大型生物样本库 BioVU， 包含与未识别的废弃样本相关的详细的未识别的人类表型信息，以及 基因型。范德比尔特疫苗中心通过有效冷冻保存的人类标本来隔离 针对 COVID-19 的单克隆抗体被开发为 Evusheld，一种预防 COVID-19 的疗法 在高危人群中。 VUMC 合作人体组织网络是国家资助的仅有的六个组织之一 癌症研究所将采购人体组织并将其分发给美国、加拿大和美国的研究人员 国际上。这些资源和其他资源提供了加速发现的引擎。统称为这些 努力提供疾病风险、疾病进展或治疗反应的有力预测因子， 支持描述疾病基本机制的研究以及临床转化。 这些大规模举措产生了大量有价值的人类和动物组织样本。这些 组织只有在液氮中才能有效冷冻保存。这些生物样本库的规模为 超出了个别研究者或团体有效策划、维持样本完整性和 快速检索样本以供进一步研究。其中许多材料无法替代，并且 国际重要性。为了满足这一巨大且不断增长的需求，专门的自动化 -190°C 低温 冷冻样品存储系统对于管理、存储和检索数以万计的样本是必要的。 生物样本库。该提案旨在获得购买 Azenta BioStore Cryo M60 的资金 机器人液氮冷冻系统，可容纳范德比尔特所有大型生物样本库。有了这样一个 系统 范德比尔特可以提供必要的机构支持、环境和专业知识，以确保成本- 对这些关键样品和试剂进行有效、有质量保证的管理、储存和检索。这 技术是耐用且可扩展的，因此可以预测该自动化系统将能够实现 广泛的研究人员，推动基础、临床和转化研究的长远发展。