Next Generation ALPHA Zebrafish Tank Washer: High Throughput with Reduced Environmental Impact
下一代 ALPHA 斑马鱼槽清洗机:高吞吐量,减少环境影响
基本信息
- 批准号:10737523
- 负责人:
- 金额:$ 22.32万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-07-01 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:AdultAnimalsBedsBiomechanicsBreedingCarbonChemicalsCongenital AbnormalityConsumptionDevelopmentDiseaseDoseEducation and OutreachElectricityEmbryoEmbryo ResearchEnsureEnvironmental ImpactEquipmentExhibitsFacultyFiltrationFishesFoodFresh WaterGenome engineeringGrantHeatingHourHousingHydrogen PeroxideLaboratoriesLaboratory ResearchLifeLiquid substanceMaintenanceManualsMicroinjectionsMicroscopeModelingModernizationMovementMusOperations ResearchOrganismOsmosisPartner in relationshipPathologicPostdoctoral FellowProductivityQuarantineResearchResearch ActivityResearch PersonnelResearch SupportRoboticsScientistServicesSourceSteamSystemTechnologyTestingTimeUnited States National Institutes of HealthUniversitiesWalkingWashingtonWaterWater PurificationZebrafishfeedingfine particlesgenetic analysisgraduate studenthuman modelimprovedmanufacturemedical schoolsmembernext generationoperationoutreach programparticleprogramsprototyperepairedsensorsperm cryopreservationultraviolet irradiationundergraduate studentwater flowwater qualityzygote
项目摘要
Zebrafish is a premier research organism for the genetic analysis of vertebrate development and modeling
human birth defects and disease. The Zebrafish Facility at Washington University School of Medicine in St. Louis
is a shared-used facility. This 5,644 sf state-of-the-art facility accommodates a total of nearly 9,000 tanks in four
animal holding rooms with independent recirculating water systems. The Facility provides optimal conditions for
the rearing and maintenance of up to 200,000 fish. Starting with four zebrafish laboratories in 2011, the Facility
currently supports research of 33 independent laboratories. Presently, over 100 graduate and undergraduate
students, postdoctoral fellows and scientists carry out research in the Facility that is supported by R00, R01,
R54, and P01 grants from the National Institutes of Health. The Facility also supports other educational and
outreach programs. The Facility enables these programs by ensuring superior husbandry with automatic feeding
that halves the time zebrafish grow to adulthood; supplying wild-type lines and embryos for experimentation,
cryopreserving sperm to minimize the number of lines maintained as life stocks, conducting pathological testing;
maintaining Quarantine System for imported lines, providing research services such as genome engineering,
and washing and disinfecting holding and breeding tanks and their components to ensure safe and efficient
research operations.
The continued increase of the zebrafish users and expansion to 9,000 tanks requires modern, technologically
advanced equipment to clean and disinfect, on a daily basis, hundreds of holding and breeding tanks, and tank
components as well as fish nets to ensure safe and efficient zebrafish research. Here we propose replacing the
existing 900 GP Walk in Washer (Tecniplast) with the next generation ALPHA Walk in Washer model
(Tecniplast). The existing 900 GP Washer is a prototype aquatics washer with several limitations: it uses steam
lines to heat water, is energetically inefficient, requires long wash cycles, uses an external chemical dosing
system, and breaks down frequently. These inefficiencies and frequent downtimes increase manual labor,
limiting and slowing down the Facility operations and research activities it supports.
The ALPHA model deploys adaptive cleaning technology that combines independent vertical and oscillating
movements allowing for superior water coverage. The ALPHA washer uses electricity to heat water, shorter
wash cycles and thus higher throughput that would meet the continually growing washing needs of the zebrafish
researchers. The reservoir for the chemicals used during wash cycles fit into the machine footprint. Operations
with the ALPHA Washer would reduce environmental impact with a shorter wash cycle and consuming smaller
amounts of chemicals per cycle. The proposed upgrade to the modern ALPHA Walk in Washer would
dramatically improve the washing capacity of zebrafish holding and breeding tanks; would meet the needs of the
growing zebrafish research operations and would also reduce the Facility’s environmental impact.
斑马鱼是脊椎动物发育和建模遗传分析的首要研究生物
人类出生缺陷和疾病。位于圣路易斯的华盛顿大学医学院的斑马鱼实验室
是一个共享的设施。这个5,644平方英尺的最先进的设施可容纳近9,000个坦克在四个
动物饲养室有独立的循环水系统。该设施提供了最佳条件,
饲养和维护多达20万条鱼。从2011年的四个斑马鱼实验室开始,该设施
目前支持33个独立实验室的研究。目前,超过100名研究生和本科生
学生、博士后研究员和科学家在R 00、R 01支持的设施中开展研究,
R54和P01赠款从美国国立卫生研究院。该基金还支持其他教育和
外展计划该设施通过确保自动饲喂的上级饲养来实现这些计划
这将使斑马鱼成年的时间减半;为实验提供野生型品系和胚胎,
冷冻保存精子,以最大限度地减少作为生命储备的品系数量,进行病理学检查;
保持进口品系的检疫制度,提供基因组工程等研究服务,
对养殖池及其组件进行清洗消毒,确保安全、高效
研究业务。
斑马鱼用户的持续增加和扩大到9,000个水箱需要现代化的技术,
先进的设备,每天清洁和消毒,数百个持有和繁殖坦克,坦克和坦克
这些设备包括组件和渔网,以确保安全和高效的斑马鱼研究。在这里,我们建议取代
现有的900 GP Walk in Washer(Tecniplast)和下一代ALPHA Walk in Washer型号
(Tecniplast).现有的900 GP洗衣机是一个原型的水产清洗机有几个限制:它使用蒸汽
加热水的管道,能量效率低,需要长时间的洗涤周期,使用外部化学剂量
系统,并经常崩溃。这些低效率和频繁的停机时间增加了体力劳动,
限制和减缓该基金的运作及其支持的研究活动。
ALPHA车型部署了自适应清洁技术,结合了独立垂直和摆动
允许上级水覆盖的运动。阿尔法洗衣机使用电力来加热水,
洗涤周期和因此更高的生产量,
研究人员清洗周期中使用的化学品的储存器适合机器占地面积。操作
与阿尔法洗衣机将减少对环境的影响,更短的洗涤周期和消耗更小
化学品每循环拟议的升级到现代阿尔法步行在洗衣机将
大大提高了斑马鱼饲养池和养殖池的清洗能力,将满足
这将有助于不断增长的斑马鱼研究业务,也将减少该基金对环境的影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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LILIANNA SOLNICAKREZEL其他文献
LILIANNA SOLNICAKREZEL的其他文献
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{{ truncateString('LILIANNA SOLNICAKREZEL', 18)}}的其他基金
Increasing the efficiency and range of prime editing for disease modeling in zebrafish
提高斑马鱼疾病建模的主要编辑效率和范围
- 批准号:
10667988 - 财政年份:2023
- 资助金额:
$ 22.32万 - 项目类别:
Society for Developmental Biology Annual Meetings 2019-2023
发育生物学学会年会 2019-2023
- 批准号:
9978076 - 财政年份:2019
- 资助金额:
$ 22.32万 - 项目类别:
Society for Developmental Biology Annual Meetings 2019-2023
发育生物学学会年会 2019-2023
- 批准号:
10653212 - 财政年份:2019
- 资助金额:
$ 22.32万 - 项目类别:
Society for Developmental Biology Annual Meetings 2019-2023
发育生物学学会年会 2019-2023
- 批准号:
10454887 - 财政年份:2019
- 资助金额:
$ 22.32万 - 项目类别:
Society for Developmental Biology Annual Meetings 2019-2023
发育生物学学会年会 2019-2023
- 批准号:
10201701 - 财政年份:2019
- 资助金额:
$ 22.32万 - 项目类别:
Inductive and morphogenetic processes shaping the zebrafish embryonic axes
塑造斑马鱼胚胎轴的诱导和形态发生过程
- 批准号:
9918423 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
Inductive and morphogenetic processes shaping the zebrafish embryonic axes
塑造斑马鱼胚胎轴的诱导和形态发生过程
- 批准号:
10614512 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
Forward and Reverse Genetic Studies of AIS and Spine Development in Zebrafish
斑马鱼 AIS 和脊柱发育的正向和反向遗传学研究
- 批准号:
10458401 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
Inductive and morphogenetic processes shaping the zebrafish embryonic axes
塑造斑马鱼胚胎轴的诱导和形态发生过程
- 批准号:
10396580 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
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