Local translation and viral infection in the airway epithelium

气道上皮的局部翻译和病毒感染

• 批准号: 10736284
• 负责人: Wellington V. Cardoso
• 金额: $ 65.23万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-09 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10736284
• 关键词: Active Sites; Address; Adult; Air; Airway Disease; Apical; Biological Products; Cell Differentiation process; Cells; Centrioles; Chimeric Proteins; Cilia; Cytoplasm; Cytoplasmic Granules; Data; Defect; Defense Mechanisms; Enzymes; Epithelium; Event; Fluorescent in Situ Hybridization; Human; Infant; Influenza A virus; Inhalation; Knowledge; Label; Liquid substance; Lung; Lung diseases; Mammalian Cell; Mediating; Messenger RNA; MicroRNAs; Morbidity - disease rate; Morphology; Mucociliary Clearance; Mus; Names; Pathogenesis; Pathologic; Pathway interactions; Peptide Initiation Factors; Phenotype; Play; Poly(A)-Binding Proteins; Production; Protein Biosynthesis; Proteins; Public Health; Puromycin; RNA immunoprecipitation sequencing; Reporting; Respiratory Syncytial Virus Infections; Respiratory System; Respiratory Tract Infections; Respiratory physiology; Respiratory syncytial virus; Ribonucleoproteins; Ribosomal Proteins; Role; Severe Acute Respiratory Syndrome; Signal Transduction; System; Testing; Translating; Translations; Trinucleotide Repeats; Viral Proteins; Virus Diseases; airway epithelium; cilium biogenesis; coronavirus disease; long-term sequelae; mRNA Transcript Degradation; mutant; novel; pathogen; pathogenic virus; polypeptide; progenitor; programs; recruit; respiratory; respiratory virus; ribosome profiling; single molecule; transcriptome

Project Summary - Abstract Multiciliated cells (MCCs) are key components of the airway epithelium playing a major role in mucociliary clearance, the first line of lung defense against inhaled pathogens. MCCs are well-known targets of viral pathogens, including influenza A, SARS-COVID2 and respiratory syncytial virus (RSV), often resulting in severe respiratory conditions with long-term sequelae and morbidity. Still there are major gaps of knowledge on the mechanisms by which these pathogens trigger airway disease. MCCs require production and apical localization of a large number of proteins essential for the assembly of hundreds of cilia per cell during multiciliogenesis. The mechanisms that allow efficient production and local translation of these proteins in MCCs are still poorly understood. There is evidence that during viral infection, the host’s cellular translation machinery is hijacked to produce viral proteins for replication. How local translation is established in MCCs and targeted in viral infection will be studied in this proposal. Our preliminary studies revealed a striking expression of translation initiation factors (eIFs), ribosomal proteins and nascent polypeptides colocalized with miRNAs, Trinucleotide repeat- containing 6a (TNRC6a) and Argonaute 2 (AGO2) in immature MCCs undergoing multiciliogenesis. These signals were concentrated in not yet reported apical cytoplasmic granules, which we named as Localized Translation granules (LT granules). Surprisingly, unlike other granules traditionally associated with miRNA function, no enzymes required for mRNA degradation were detected in LT granules. Instead, LT granules were highly active sites of protein translation. Notably, disrupting Tnrc6a expression, disturbed local translation and resulted in defective multicilia formation, a phenotype we also observed in RSV-infected MCCs. Here we will test the hypotheses that i) components of the miRNA pathway recruit subsets of mRNAs and the translation machinery to LT granules for local efficient large-scale protein synthesis during MCC differentiation; ii) disruption of this local translation program is a key determinant in the pathogenesis of RSV infection. Thus, we propose to identify mechanisms that target mRNAs (Aim 1) and recruit the translation machinery (Aim 2) to LT granules in MCCs, and determine how RSV disrupts these mechanisms to infect the human airway epithelium (Aim 3). The knowledge generated from these studies will significantly advance our understanding of the mechanisms of local translation in multiciliogenesis and the pathogenesis of RSV infection in the respiratory tract. RELEVANCE TO PUBLIC HEALTH: Multiciliated cells (MCCs) are crucial components of the defense mechanisms of the lung. Viral infections in the immature epithelium of conducting airways can have devastating effects in infants or adults with compromised respiratory function. Novel information generated from these studies will advance our understanding of how proper ciliogenesis is regulated, and how viral infections, such as RSV disrupt cilia formation in immature respiratory tract.

项目摘要-摘要