Identification of Free Radical Induced Biomarkers of Exposure to Electronic Cigarette Aerosol
暴露于电子烟气溶胶的自由基诱导生物标志物的鉴定
基本信息
- 批准号:10771404
- 负责人:
- 金额:$ 24.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-04-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAdoptionAerosolsAnimalsAntibodiesAreaAwardBiologicalBiological MarkersC57BL/6 MouseCharacteristicsChemicalsChronic Obstructive Pulmonary DiseaseDNA AdductionDNA AdductsDataDetectionDevelopmentDiseaseElectron Spin Resonance SpectroscopyElectronic cigaretteEnsureExposure toFree RadicalsFreezingGlycerolHarm ReductionHealthInflammationLong-Term EffectsLungMalignant NeoplasmsMass Spectrum AnalysisMeasuresMentorshipModelingMusNoseOxidantsOxidesPathway interactionsPhasePhysiologic pulseProliferatingPropylene GlycolsProteinsReactionResearchResearch PersonnelRodentRodent ModelSafetyScienceSeriesSerumSolventsSpin TrappingStructureTechniquesTimeTissuesTobaccoTobacco smokeTrainingTranslational ResearchWorkYouthadductassaultbiomarker developmente-cigarette aerosolselectronic cigarette useelectronic liquidenvironmental tobacco smoke exposureexperiencein vivoinsightmetabolomicsnoveloxidative damagepyrrolinespecific biomarkerssymposiumtobacco regulatory sciencetooltoxicanttumor progressionvaping
项目摘要
Project Summary/Abstract: Electronic cigarette (e-cig) usage is on the rise, particularly among youths;
however, their potential for harm is not understood, complicating development of informed regulatory strategies.
The lack of data on e-cig related harm is, in part, due to the lack of specific biomarkers for exposure to e-cig
aerosols. We found that e-cig aerosol contains highly reactive free radicals that can cause oxidative damage to
the user. Free radicals can damage numerous cellular pathways possibly contributing to the progression of
cancers and other diseases. Detection of e-cig free radicals can be accomplished by trapping with spin traps
(e.g., DMPO) and analysis by electron paramagnetic resonance (EPR) spectroscopy. Preliminary research with
EPR shows that free radicals produced in the e-cig aerosol by e-liquid solvents, propylene glycol (PG) and
glycerin (GLY), common to all e-cigs, display unique structural characteristics. Our objective is to identify these
free radical structures and utilize their unique structure to develop an e-cig specific biomarker of exposure. The
specific aims of the proposed research are: (Aim 1) To determine the structures of the free radicals produced
by PG and GLY in e-cigs; (Aim 2) To determine the primary targets of and adducts formed from free radical
assault in the tissue of e-cig exposures in rodent models and possible metabolites formed from the these radical
adducts in the serum of e-cig exposed rodent. This project represents an important research direction where a
chemical/biological approach can inform tobacco regulatory science. As such, an important aspect of this
application it to extend my background in areas relevant to translational science in addition to providing specific
training in new biomarker-relevant research areas including metabolomics and free radical structural
identification. To this end, my training will occur through a series of courses, relevant mentorship, and practical
experience, each geared to ensure my transition to an independent researcher in the fields of biomarker
development and regulatory science. Coursework, mentorship, conference participation, and practical
training/experience will be completed during the K99 phase. During this phase, Aim 1 of the research plan will
be completed and Aim 2 will be initiated (for completion during the R00 phase of the award). To accomplish the
research aims, advanced pulsed EPR and mass spectroscopy approaches will be utilized for radicals produced
by PG and GLY in e-cig aerosols. In a mouse exposure model, free radical exposure and targets of attack in
the lung will be determined using a novel in vivo DMPO/anti-DMPO antibody approach. This will allow for the
identification of specific protein and DNA adducts by TOF-MS. A post-DMPO exposure study will consist of an
untargeted pairwise metabolomics approach to look at changes in metabolite profiles before and after e-cig
exposure. By leveraging the unique structures of e-cig produced free radicals and their targets of attack in the
lung, biomarkers of exposure specific to e-cig aerosols can be identified and used to develop regulatory
strategies aimed at reducing harm from e-cig exposure.
项目摘要/摘要:电子烟(e- cigg)的使用量呈上升趋势,尤其是在青少年中;
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Zachary T Bitzer其他文献
Zachary T Bitzer的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Zachary T Bitzer', 18)}}的其他基金
Electronic Cigarette-derived Oxidants and their Impact on Lung Cancer Development
电子烟衍生的氧化剂及其对肺癌发展的影响
- 批准号:
10631182 - 财政年份:2020
- 资助金额:
$ 24.9万 - 项目类别:
Identification of Free Radical Induced Biomarkers of Exposure to Electronic Cigarette Aerosol
暴露于电子烟气溶胶的自由基诱导生物标志物的鉴定
- 批准号:
9981091 - 财政年份:2020
- 资助金额:
$ 24.9万 - 项目类别:
Electronic Cigarette-derived Oxidants and their Impact on Lung Cancer Development
电子烟衍生的氧化剂及其对肺癌发展的影响
- 批准号:
10413133 - 财政年份:2020
- 资助金额:
$ 24.9万 - 项目类别:
Identification of Free Radical Induced Biomarkers of Exposure to Electronic Cigarette Aerosol
暴露于电子烟气溶胶的自由基诱导生物标志物的鉴定
- 批准号:
10363128 - 财政年份:2020
- 资助金额:
$ 24.9万 - 项目类别:
Oxidant Exposure and Related Harm from Tobacco Smoke
烟草烟雾中的氧化剂暴露和相关危害
- 批准号:
10191024 - 财政年份:2019
- 资助金额:
$ 24.9万 - 项目类别:
相似海外基金
WELL-CALF: optimising accuracy for commercial adoption
WELL-CALF:优化商业采用的准确性
- 批准号:
10093543 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
Collaborative R&D
Investigating the Adoption, Actual Usage, and Outcomes of Enterprise Collaboration Systems in Remote Work Settings.
调查远程工作环境中企业协作系统的采用、实际使用和结果。
- 批准号:
24K16436 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Unraveling the Dynamics of International Accounting: Exploring the Impact of IFRS Adoption on Firms' Financial Reporting and Business Strategies
揭示国际会计的动态:探索采用 IFRS 对公司财务报告和业务战略的影响
- 批准号:
24K16488 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10107647 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
EU-Funded
Assessing the Coordination of Electric Vehicle Adoption on Urban Energy Transition: A Geospatial Machine Learning Framework
评估电动汽车采用对城市能源转型的协调:地理空间机器学习框架
- 批准号:
24K20973 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
- 批准号:
10106221 - 财政年份:2024
- 资助金额:
$ 24.9万 - 项目类别:
EU-Funded
Our focus for this project is accelerating the development and adoption of resource efficient solutions like fashion rental through technological advancement, addressing longer in use and reuse
我们该项目的重点是通过技术进步加快时装租赁等资源高效解决方案的开发和采用,解决更长的使用和重复使用问题
- 批准号:
10075502 - 财政年份:2023
- 资助金额:
$ 24.9万 - 项目类别:
Grant for R&D
Engage2innovate – Enhancing security solution design, adoption and impact through effective engagement and social innovation (E2i)
Engage2innovate — 通过有效参与和社会创新增强安全解决方案的设计、采用和影响 (E2i)
- 批准号:
10089082 - 财政年份:2023
- 资助金额:
$ 24.9万 - 项目类别:
EU-Funded
De-Adoption Beta-Blockers in patients with stable ischemic heart disease without REduced LV ejection fraction, ongoing Ischemia, or Arrhythmias: a randomized Trial with blinded Endpoints (ABbreviate)
在没有左心室射血分数降低、持续性缺血或心律失常的稳定型缺血性心脏病患者中停用β受体阻滞剂:一项盲法终点随机试验(ABbreviate)
- 批准号:
481560 - 财政年份:2023
- 资助金额:
$ 24.9万 - 项目类别:
Operating Grants
Collaborative Research: SCIPE: CyberInfrastructure Professionals InnoVating and brOadening the adoption of advanced Technologies (CI PIVOT)
合作研究:SCIPE:网络基础设施专业人员创新和扩大先进技术的采用 (CI PIVOT)
- 批准号:
2321091 - 财政年份:2023
- 资助金额:
$ 24.9万 - 项目类别:
Standard Grant