Multi-analyte Approach for Earlier Detection of Cancers in Non Plasma Biofluids

用于早期检测非血浆生物流体中癌症的多分析物方法

• 批准号: 10763308
• 负责人: Nishant Agrawal
• 金额: $ 98.71万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-19 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10763308
• 关键词: Achievement; Affect; Agreement; Aneuploidy; Area; Bioinformatics; Biological Assay; Biology; Blood; Brain; CLIA certified; Cancer Detection; Cerebrospinal Fluid; Cessation of life; Circulation; Clinical; Coin; Collaborations; DNA; Data; Detection; Diagnosis; Diagnostic; Early Diagnosis; Epigenetic Process; Funding; Genomic Library; Goals; Head and Neck Cancer; Head and neck structure; Individual; Industrialization; Industry Collaboration; Industry Standard; Intervention Trial; Libraries; Liquid substance; Logistic Regressions; Malignant - descriptor; Malignant Neoplasms; Malignant neoplasm of brain; Medical; Methods; Methylation; Modality; Modeling; Molecular; Morbidity - disease rate; Mutation; Neoplasms; Organ; Outcome; Output; Performance; Plasma; Procedures; Process; Protein Secretion; Protocols documentation; Quality Control; Saliva; Screening for cancer; Signal Transduction; Site; Specificity; Target Populations; Technology; Testing; Translating; Tumor-Derived; Validation; Woman; Work; brain abnormalities; brain magnetic resonance imaging; burden of illness; cancer biomarkers; cancer cell; cancer diagnosis; cancer risk; cancer type; case control; clinical application; clinical implementation; clinical translation; cohort; cost; detection assay; experience; improved; industry partner; liquid biopsy; machine learning algorithm; member; minimally invasive; mortality; neoplastic; novel; oral lesion; parent grant; prospective; sample collection; screening; specific biomarkers; standard of care; tumor; tumor DNA

PROJECT SUMMARY Early detection of cancer provides one of the most effective ways to reduce cancer related morbidity and mortality. However, most malignancies including brain and head and neck cancers do not have effective minimally invasive strategies for early detection. Unfortunately, early detection is hindered by several factors, including the lack of suitable tests and cost. One of the chief challenges in developing non-invasive tests is the identification of the appropriate biofluid and specific markers. While blood-based testing has shown promise, many cancers are not easily detected in the circulation. Over the past two decades, DNA released from cancer cells has emerged as a specific biomarker of cancer. We have previously developed sensitive methods for the simultaneous detection of multiple DNA based analytes from a small quantity of starting material in a variety of biospecimens. We have extensive preliminary results that biofluids, such as cerebrospinal fluid (CSF) and saliva, associated with certain cancers are significantly enriched in released tumor DNA (rtDNA) compared to blood. In addition, our group has an ongoing relationship with an industrial partner to translate our promising technology into the clinical realm. This proposal will build on those strengths to develop assays for the sensitive and specific detection of brain and head and neck cancer in CSF and saliva, respectively. A non-plasma based “liquid biopsy” approach for the earlier detection of cancers difficult to detect in the blood would represent a significant medical advance.

项目概要 癌症的早期检测提供了减少癌症相关的最有效方法之一 发病率和死亡率。然而，大多数恶性肿瘤，包括脑部和头颈部肿瘤 癌症没有有效的早期检测微创策略。很遗憾， 早期检测受到多种因素的阻碍，包括缺乏适当的测试和成本。一 开发非侵入性检测的主要挑战之一是确定适当的方法 生物流体和特异性标记物。虽然基于血液的检测已显示出希望，但许多癌症 在循环中不易被检测到。过去二十年来，癌症释放的DNA 细胞已成为癌症的特异性生物标志物。我们之前开发过敏感 从少量样品中同时检测多种基于 DNA 的分析物的方法 各种生物样本的起始材料。我们有广泛的初步结果 与某些癌症相关的生物体液，例如脑脊液 (CSF) 和唾液 与血液相比，释放的肿瘤 DNA (rtDNA) 显着富集。另外，我们组 与工业合作伙伴保持着持续的合作关系，将我们有前景的技术转化为 临床领域。该提案将建立在这些优势的基础上，开发用于 灵敏且特异地检测脑脊液和唾液中的脑癌和头颈癌， 分别。用于早期癌症检测的非血浆“液体活检”方法 很难在血液中检测到，这将代表着重大的医学进步。

项目成果

Response rate and molecular correlates to encorafenib and binimetinib in BRAF-V600E mutant high-grade glioma.

BRAF-V600E 突变型高级别胶质瘤中恩科拉非尼和 binimetinib 的缓解率和分子相关性。

• DOI: 10.1158/1078-0432.ccr-23-3241
• 发表时间: 2024
• 期刊: Clinical cancer research : an official journal of the American Association for Cancer Research
• 作者: Schreck,KarisaC;Strowd,RoyE;Nabors,LouisB;Ellingson,BenjaminM;Chang,Michael;Tan,SzeK;Abdullaev,Zied;Turakulov,Rust;Aldape,Kenneth;Danda,Neeraja;Desideri,Serena;Fisher,Joy;Iacoboni,Michaella;Surakus,Trisha;Rudek,MichelleA
• 通讯作者: Rudek,MichelleA