TAKASHIMA DIVERSITY SUPPLEMENT TO: REGULATION OF GAMETE USE AND NEURAL PATHWAYS IN REPRODUCTION

高岛多样性补充：配子使用和生殖神经通路的调节

• 批准号: 10764479
• 负责人: ANDREW G CLARK
• 金额: $ 8.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-24 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10764479
• 关键词: Academic Training; Affect; Area; Biological Assay; Biology; Data; Development; Doctor of Philosophy; Drosophila genus; Educational process of instructing; Educational workshop; Ejaculation; Encapsulated; Ethics; Female; Fertilization; Germ Cells; Goals; Immunofluorescence Microscopy; Latina; Learning; Lipid Binding; Mammals; Mentors; Modeling; Neural Pathways; Partner in relationship; Phenotype; Process; Production; Proteins; RNA; Regulation; Reproduction; Research; Research Training; Resources; Science; Seminal; Statistical Data Interpretation; Statistical Models; System; Training; Variant; Vesicle; Work; career; career development; doctoral student; exosome; experience; father role; genetic analysis; genetic variant; genome wide association study; graduate student; interest; male; meetings; reproductive; reproductive success; response; skills; sperm cell; student mentoring; symposium

Project Summary This is a Diversity Supplement to support the development of the career of Yoko Takashima, a second year PhD student who is engaged in work related to R01 HD059060, “Regulation of Gamete Use and Neural Pathways in Reproduction”. Her work focuses on three primary aims related to the determinants of reproductive success. The first aim is to elucidate the functional consequences of the transfer of exosomes (vesicles) from males to females during mating, a phenomenon seen in mammals and in Drosophila. The function of these transferred exosomes, minute lipid-bound vesicles that encapsulate and transfer proteins and RNAs, is not well- understood in any system. In Drosophila they have been suggested to affect the process of sperm competition, in which ejaculates from successive males compete for fertilization opportunities. Ms. Takashima will assess the variation in exosome production, transfer, and its effects on sperm competition, allowing her to apply genome-wide association (GWAS) approaches to identify previously unknown genetic variants that impact exosome biology. In her second aim, Ms. Takashima will dissect the mechanism(s) employed by exosome contents to impact female post-mating responses, including a specific assay of sperm competitive ability. Finally, in Aim 3, these data will be incorporated into a promising statistical modeling approach, the Bradley-Terry model, to quantify the effects of perturbed seminal exosomes on male competitive-reproductive success. We have devised a detailed mentoring plan that will provide Ms. Takashima tailored support for both her scientific development and to guide her in achieving her career goals. She will receive academic and research training, training in presentational skills and responsible conduct in research, teaching/mentoring experience, and guidance to complete an impactful PhD. She will also receive additional guidance to help her choose among career options that she is considering; this will involve one-on-one guidance from her mentors, networking that we will set up for her with colleagues and alumni in careers she is exploring, as well as workshops and resources at Cornell and at conferences she will attend.

项目摘要 这是一份支持高岛洋子事业发展的多元化补充资料， 从事R01 HD059060相关工作的二年级博士生，《规定》 生殖中配子的使用和神经通路“。她的工作集中在三个主要目标上 与生殖成功的决定因素有关。第一个目标是阐明功能 交配过程中从雄性向雌性转移外体(囊泡)的后果 哺乳动物和果蝇身上的现象。这些被转移的外切体的功能， 包裹和运输蛋白质和RNA的微小脂质结合囊泡不是很好- 在任何系统中都能理解。在果蝇中，它们被认为影响了 精子竞争，即连续从雄性射精的精子竞争受精 机遇。Takashima女士将评估外切体生产、转移和其 对精子竞争的影响，使她能够应用全基因组关联(GWAS) 识别影响外显体生物学的以前未知的遗传变异的方法。在她身上 第二个目的，高岛女士将剖析exosome Contents使用的机制(S) 影响雌性交配后的反应，包括精子竞争能力的特定测试。 最后，在目标3中，这些数据将被纳入一个有希望的统计建模方法， Bradley-Terry模型，量化受扰的精液外精体对男性的影响 竞争-繁殖的成功。我们已经制定了详细的指导计划，将提供 高岛女士为她的科学发展量身定做了支持，并指导她实现 她的职业目标。她将接受学术和研究培训，演示培训 在研究、教学/指导经验和指导方面的技能和负责任的行为 完成一个有影响力的博士学位。她还将接受额外的指导，帮助她在 她正在考虑的职业选择；这将涉及她的导师的一对一指导， 我们将为她与她正在探索的职业领域的同事和校友建立联系，因为 以及康奈尔大学的研讨会和资源，以及她将参加的会议。