Imaging spatial transcriptome at single cell resolution to study eye regeneration in old age

以单细胞分辨率对空间转录组进行成像以研究老年眼再生

• 批准号: 10785595
• 负责人: Longhua Guo
• 金额: $ 19.5万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-30 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10785595
• 关键词: Age; Age related macular degeneration; Aging; Animal Model; Animals; Architecture; Atlases; Body part; Cell Communication; Cells; Collaborations; Data; Data Set; Development; Disease; Elderly; Eye; Fresh Water; Gene Expression; Genes; Goals; Head; Image; Impairment; Knowledge; Laboratory culture; Learning; Methods; Molecular; Natural regeneration; Output; Pathway interactions; Patients; Pattern; Persons; Phenotype; Pigments; Planarians; Platyhelminths; Process; Production; Quality of life; Regenerative Medicine; Regenerative capacity; Regulation; Rejuvenation; Reporting; Research; Resolution; Signal Transduction; Slide; Technology; Therapeutic; Tissues; Work; adult stem cell; age related; aged; cell type; differential expression; empowerment; experimental study; eye regeneration; fighting; human old age (65+); improved; molecular dynamics; next generation; older patient; progenitor; regenerative; repaired; single cell mRNA sequencing; stem cell biology; stem cell function; stem cell technology; stem cells; theories; tissue regeneration; tool; transcriptome; transcriptomics

ABSTRACT To cure age-related diseases is of critical importance for the life quality of elderly people. One promising strategy is to slow down aging or reverse aging with regenerative medicine. However, the regenerative capacity of stem cells and their supportive microenvironments are drastically impaired in old age. Hence, to rejuvenate functions of aged adult stem cells and their microenvironments or to maintain the youthful functions of adult stem cells in old age are critical research directions to empower regenerative medicine to fight age-associated diseases. Here, we propose to use a cutting-edge spatial transcriptomics method, Seq-Scope, to comprehensively examine the aging, regeneration and rejuvenation of eye progenitor cells and their niche cells in the sexual planarians, a champion of tissue regeneration. We aim to determine the molecular pathways that facilitate regeneration and rejuvenation in old age. The proposed experiments will define datasets of fundamental importance in the identification of niche cells and changes in aging and regeneration that will fuel long-term mechanistic studies.

摘要 老年病的治疗对老年人的生活质量至关重要。一个很有前途的策略 就是用再生医学来减缓衰老或者逆转衰老。然而，茎的再生能力 细胞及其支持微环境在老年时严重受损。因此，为了恢复功能， 或维持成体干细胞的年轻功能， 老年是关键的研究方向，使再生医学能够对抗与年龄相关的疾病。在这里， 我们建议使用尖端的空间转录组学方法Seq-Scope来全面检查 性真涡虫眼祖细胞及其生态位细胞的衰老、再生和复壮， 组织再生的冠军我们的目标是确定促进再生的分子途径， 在年老时恢复活力。拟议的实验将定义数据集的根本重要性， 识别小生境细胞以及老化和再生的变化，这将推动长期的机制研究。