Imaging spatial transcriptome at single cell resolution to study eye regeneration in old age

摘要 老年病的治疗对老年人的生活质量至关重要。一个很有前途的策略 就是用再生医学来减缓衰老或者逆转衰老。然而，茎的再生能力 细胞及其支持微环境在老年时严重受损。因此，为了恢复功能， 或维持成体干细胞的年轻功能， 老年是关键的研究方向，使再生医学能够对抗与年龄相关的疾病。在这里， 我们建议使用尖端的空间转录组学方法Seq-Scope来全面检查 性真涡虫眼祖细胞及其生态位细胞的衰老、再生和复壮， 组织再生的冠军。我们的目标是确定促进再生的分子途径， 在年老时恢复活力。拟议的实验将定义数据集的根本重要性， 识别小生境细胞以及老化和再生的变化，这将推动长期的机制研究。

ABSTRACT To cure age-related diseases is of critical importance for the life quality of elderly people. One promising strategy is to slow down aging or reverse aging with regenerative medicine. However, the regenerative capacity of stem cells and their supportive microenvironments are drastically impaired in old age. Hence, to rejuvenate functions of aged adult stem cells and their microenvironments or to maintain the youthful functions of adult stem cells in old age are critical research directions to empower regenerative medicine to fight age-associated diseases. Here, we propose to use a cutting-edge spatial transcriptomics method, Seq-Scope, to comprehensively examine the aging, regeneration and rejuvenation of eye progenitor cells and their niche cells in the sexual planarians, a champion of tissue regeneration. We aim to determine the molecular pathways that facilitate regeneration and rejuvenation in old age. The proposed experiments will define datasets of fundamental importance in the identification of niche cells and changes in aging and regeneration that will fuel long-term mechanistic studies.