A BABOON MODEL OF DEGENERATIVE DISC DISEASE: A PILOT STUDY TO EVALUATE

椎间盘退变性疾病的狒狒模型：一项评估试点研究

• 批准号: 7562453
• 负责人: Stephen F Butler
• 金额: $ 0.33万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562453
• 关键词: Acute; Adult; Back; Biomechanics; Cartilage; Characteristics; Chronic; Computer Retrieval of Information on Scientific Projects Database; Disease; Functional disorder; Funding; Gene Chips; Genetic; Grant; Histology; Human; Hydrogels; Implant; Injury; Institution; Intervertebral disc structure; Literature; Lumbar Disc Degenerative Disorder; Magnetic Resonance Imaging; Mammals; Miniature Swine; Modeling; Monitor; Monkeys; Movement; Mus; Neck Pain; Operative Surgical Procedures; Oryctolagus cuniculus; Papio; Pattern; Pilot Projects; Primates; Process; Rattus; Reporting; Research; Research Personnel; Resources; Sheep; Source; Structure; Techniques; Technology; Time; Tissues; United States National Institutes of Health; Vascular blood supply; Vertebral column; Water; bone; in vivo Model; repaired; response; size; spine bone structure; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Vertebral discs are cushions of cartilage between each bone of the spine allowing for movement. An outer ring of tough fibrous cartilage that surrounds an inner water-filled center forms the discs. Discs are the largest structures in the body without a blood supply. Because of this, damaged or degenerated disc tissue is extremely limited in its ability to repair itself. An estimated 5 million adults in the US alone suffer with chronic back or neck pain primarily due to disc degeneration. There is no proven model for induced lumbar disc degeneration in the baboon, and research has generally been limited to normal spines with acute injury. This study will develop a degenerated lumbar disc model in the baboon. A proven model would become an invaluable tool for the study of the degenerated disc and its treatment with implants like dynamic stabilization and biologics like hydrogels. The results of this study will be used to determine that surgical technique and time point which best produce mild-to-moderate degenerative disc disease in people. It is hypothesized that an appropriate injury to the disc will induce a reproducible degenerative response that replicates degenerative disc disease in the human lumbar spine. The literature reports the use of mice, rats, rabbits, sheep, minipigs, monkeys, and baboons as in vivo models for study of the lumbar spine. The primate model better reproduces the biomechanics of an erect spine than the sheep model. Compared to smaller mammals, the baboon may better reproduce the pathophysiology of the human spine because of its relatively large size. In fact, the intervertebral disc of the baboon demonstrates characteristic patterns of degeneration similar to those in humans. Additionally, genetic homology between humans and primates may also facilitate analyses using immunohistochemical and gene chip technologies. In this pilot study, six baboons will undergo disc surgery and two different types of injury will be caused at three different levels. The degeneration will be monitored with radiological and magnetic resonance imaging. After the baboons are euthanized at the end of the study, the spines will be processed for histology.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 椎盘是脊椎每根骨头之间的软骨垫，允许运动。由坚韧的纤维软骨组成的外环环绕着内部充满水的中心，形成了椎间盘。椎间盘是体内最大的结构，没有血液供应。正因为如此，受损或退化的椎间盘组织自我修复的能力极其有限。据估计，仅在美国就有500万成年人患有慢性背部或颈部疼痛，主要原因是椎间盘退变。 目前还没有得到证实的在狒狒身上诱导腰椎间盘退行性变的模型，研究通常局限于有急性损伤的正常脊柱。这项研究将在狒狒身上建立一个退变的腰椎间盘模型。经过验证的模型将成为研究退变的椎间盘及其植入物(如动态稳定器)和生物制剂(如水凝胶)治疗的无价工具。这项研究的结果将被用来确定在人类中最容易产生轻度至中度退行性腰椎间盘疾病的手术技术和时间点。据推测，对椎间盘的适当损伤将引起可重复的退变反应，从而在人类腰椎复制退行性腰椎间盘疾病。 文献报道使用小鼠、大鼠、兔子、绵羊、小型猪、猴子和狒狒作为腰椎研究的活体模型。灵长类动物模型比绵羊模型更好地再现了直立脊柱的生物力学。与体型较小的哺乳动物相比，由于其体型相对较大，它可能会更好地再现人类脊椎的病理生理。事实上，狒狒的椎间盘表现出与人类相似的典型退变模式。此外，人类和灵长类动物之间的基因同源性也可能有助于使用免疫组织化学和基因芯片技术进行分析。 在这项初步研究中，六只狒狒将接受椎间盘手术，并将在三个不同的水平造成两种不同类型的伤害。退化将通过放射和磁共振成像进行监测。在研究结束时对狒狒实施安乐死后，将对脊椎进行组织学处理。