A BABOON MODEL OF DEGENERATIVE DISC DISEASE: A PILOT STUDY TO EVALUATE

椎间盘退变性疾病的狒狒模型：一项评估试点研究

• 批准号: 7562453
• 负责人: Stephen F Butler
• 金额: $ 0.33万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562453
• 关键词: Acute; Adult; Back; Biomechanics; Cartilage; Characteristics; Chronic; Computer Retrieval of Information on Scientific Projects Database; Disease; Functional disorder; Funding; Gene Chips; Genetic; Grant; Histology; Human; Hydrogels; Implant; Injury; Institution; Intervertebral disc structure; Literature; Lumbar Disc Degenerative Disorder; Magnetic Resonance Imaging; Mammals; Miniature Swine; Modeling; Monitor; Monkeys; Movement; Mus; Neck Pain; Operative Surgical Procedures; Oryctolagus cuniculus; Papio; Pattern; Pilot Projects; Primates; Process; Rattus; Reporting; Research; Research Personnel; Resources; Sheep; Source; Structure; Techniques; Technology; Time; Tissues; United States National Institutes of Health; Vascular blood supply; Vertebral column; Water; bone; in vivo Model; repaired; response; size; spine bone structure; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Vertebral discs are cushions of cartilage between each bone of the spine allowing for movement. An outer ring of tough fibrous cartilage that surrounds an inner water-filled center forms the discs. Discs are the largest structures in the body without a blood supply. Because of this, damaged or degenerated disc tissue is extremely limited in its ability to repair itself. An estimated 5 million adults in the US alone suffer with chronic back or neck pain primarily due to disc degeneration. There is no proven model for induced lumbar disc degeneration in the baboon, and research has generally been limited to normal spines with acute injury. This study will develop a degenerated lumbar disc model in the baboon. A proven model would become an invaluable tool for the study of the degenerated disc and its treatment with implants like dynamic stabilization and biologics like hydrogels. The results of this study will be used to determine that surgical technique and time point which best produce mild-to-moderate degenerative disc disease in people. It is hypothesized that an appropriate injury to the disc will induce a reproducible degenerative response that replicates degenerative disc disease in the human lumbar spine. The literature reports the use of mice, rats, rabbits, sheep, minipigs, monkeys, and baboons as in vivo models for study of the lumbar spine. The primate model better reproduces the biomechanics of an erect spine than the sheep model. Compared to smaller mammals, the baboon may better reproduce the pathophysiology of the human spine because of its relatively large size. In fact, the intervertebral disc of the baboon demonstrates characteristic patterns of degeneration similar to those in humans. Additionally, genetic homology between humans and primates may also facilitate analyses using immunohistochemical and gene chip technologies. In this pilot study, six baboons will undergo disc surgery and two different types of injury will be caused at three different levels. The degeneration will be monitored with radiological and magnetic resonance imaging. After the baboons are euthanized at the end of the study, the spines will be processed for histology.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 椎骨椎间盘是脊柱的每个骨头之间的软骨垫子，允许运动。围绕着内水中心的坚韧纤维软骨的外环形成圆盘。 椎间盘是体内最大的结构，没有血液供应。因此，受损或退化的盘组织的修复能力极为有限。 仅在美国，估计有500万成年人患有慢性背部或颈部疼痛，主要是由于椎间盘变性。 狒狒中没有诱发腰椎椎间盘变性的良好模型，并且研究通常仅限于正常的棘突。这项研究将在狒狒中开发退化的腰椎模型。一个经过验证的模型将成为研究退化椎间盘的宝贵工具及其对动态稳定和水凝胶等生物制剂等植入物的处理。这项研究的结果将用于确定最能在人们中产生轻度到中度退行性椎间盘疾病的手术技术和时间点。假设对椎间盘的适当损伤将引起可重复的退行性反应，从而复制人类腰椎的退行性椎间盘疾病。 文献报告说，将小鼠，大鼠，兔子，绵羊，迷你鸡，猴子和狒狒用作体内模型进行腰椎研究。与绵羊模型相比，灵长类动物模型可以更好地再现直立脊柱的生物力学。与较小的哺乳动物相比，狒狒可能会更好地再现人脊柱的病理生物生物生物生物生理，因为它的尺寸相对较大。实际上，狒狒的椎间盘表现出与人类类似的变性模式。另外，人类和灵长类动物之间的遗传同源性也可能促进使用免疫组织化学和基因芯片技术进行分析。 在这项试点研究中，将六个狒狒接受椎间盘手术，并在三个不同的水平下造成两种不同类型的损伤。退化将通过放射学和磁共振成像进行监测。 在研究结束时，狒狒安乐死后，将处理脊柱以进行组织学。