CD8 T cell Immunity to Influenza

CD8 T 细胞对流感的免疫

• 批准号: 7657178
• 负责人: Ann Arvin
• 金额: $ 16.19万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7657178
• 关键词: Acute; Address; Adhesions; Adult; Age; Animal Model; Antigens; Antiviral Agents; Attenuated; Attenuated Live Virus Vaccine; Attenuated Vaccines; B-Lymphocytes; Blood specimen; CD28 gene; CD4 Positive T Lymphocytes; CD8B1 gene; Cell physiology; Characteristics; Child; Clinical Research; Color; Data Set; Detection; Elderly; Evaluation; Event; Exposure to; Flow Cytometry; Frequencies; Human; Immune; Immune response; Immunity; Immunization; Immunology; Individual; Infection; Influenza; Influenza A virus; Investigation; Knowledge; Life; Medical Surveillance; Memory; Methods; NCAM1 gene; Nose; Numbers; Pattern; Phenotype; Play; Polymerase Chain Reaction; Population; Predisposition; Production; Research Project Grants; Risk; Role; Seasons; T memory cell; T-Lymphocyte; Techniques; Testing; Thinking; Time; Translational Research; Vaccination; Vaccines; Virus; Virus Diseases; Week; age group; base; biodefense; chemokine receptor; cohort; human subject; influenza virus vaccine; pandemic disease; pathogen; peripheral blood; prospective; research study; response; tool; young adult

The objective of this project is to acquire new knowledge about the role of the CD8 T cells in protecting against influenza infection. The CD8 T cell response is thought to play a pivotal role in clearing infection and establishing protective immunity from studies in animal models. However, the quantitative and qualitative characteristics of the influenza A virus-specific CD8 T cell response to natural infection and vaccines have not been investigated systematically in the human host. We propose to evaluate influenza A virus-specific CD8 T cells in child populations and adults. Experiments will use multi-color flow cytometry-based techniques which overcome technical obstacles to assessing CD8 T cell immunity in human populations, including children, to characterize CD8 T cells specific for influenza A antigens. Prospective evaluations will be done in all age cohorts who are immunized with inactivated or live attenuated influenza vaccines and in children with natural influenza A infection. The Specific Aims are: 1. To characterize the influenza A-specific CD8 T cell response before and after administration of inactivated influenza vaccine or live attenuated influenza vaccine to children, young adults, and older adults. We hypothesize that immunization will induce effector and memory CD8 T cell responses at different time points, as characterized by specific patterns of multiple phenotypes and T cell functions. We expect responses to differ by vaccine type, and age. 2. To characterize the number, phenotype and functions of influenza A virus-specific effector and memory CD8 T cells in children with acute influenza. To evaluate antiviral CD8 T cell responses in children allows study of the primary CD8 T cell response in naTve exposed, subjects. We hypothesize that CD8 T cell responses induced by natural infection will resemble responses to live vaccine. This project is a component of our coordinated efforts addressing T cell and B cell responses as well as natural killer responses to influenza A using the same cohort of human subjects and will provide new information on the CD8 T cell immunity in the context of the overall host responses to influenza A. This information will be critical for providing enhanced protection during a potential influenza A pandemic while it is naturally occurring or a bioterrorist event.

该项目的目的是获得有关CD8 T细胞在保护免疫系统中的作用的新知识。 预防流感感染。CD8 T细胞应答被认为在清除 感染和建立动物模型研究的保护性免疫。但 甲型流感病毒特异性CD8 T细胞应答的定量和定性特征 自然感染和疫苗尚未在人类宿主中进行系统研究。我们 建议评估儿童和成人中甲型流感病毒特异性CD8 T细胞。实验 将使用多色流式细胞术为基础的技术，克服技术障碍，评估 包括儿童在内的人群中的CD8 T细胞免疫，以表征CD8 T细胞特异性 甲型流感抗原。将在所有年龄组中进行前瞻性评价， 灭活或减毒活流感疫苗和自然感染甲型流感的儿童。的 具体目标是：1。表征在使用前和后的甲型流感特异性CD8 T细胞应答 对儿童、青少年和儿童接种灭活流感疫苗或减毒活流感疫苗 成年人和老年人。我们假设免疫将诱导效应和记忆CD8 T细胞， 在不同时间点的反应，其特征在于多种表型和T 细胞功能。我们预计反应会因疫苗类型和年龄而异。2.为了描述这个数字， 甲型流感病毒特异性效应和记忆CD8 T细胞的表型和功能 急性流感为了评估儿童抗病毒CD8 T细胞应答，可以研究原发性CD8 T细胞 未暴露受试者中的T细胞应答。我们假设，CD8诱导的T细胞反应， 自然感染将类似于对活疫苗的反应。该项目是我们协调的一个组成部分 致力于解决T细胞和B细胞应答以及自然杀伤细胞对甲型流感的应答， 同一组人类受试者，并将提供关于CD8 T细胞免疫的新信息。 宿主对甲型流感总体反应的背景。这些信息对于提供 在潜在的甲型流感大流行期间加强保护，而它是自然发生的， 生物恐怖事件