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Histology

组织学

基本信息

批准号：
7746112
负责人：
Sun-Sang Joseph Sung
金额：
$ 12.74万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-18 至 2014-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7746112
关键词：
Adenovirus Infections Adenoviruses Affinity Animals Antigen-Presenting Cells Applications Grants Binding CD8 receptor CD8B1 gene Cell Adhesion Molecules Cell Communication Cell physiology Cell surface Cells Color Competence Confocal Microscopy Core Facility Cytokine Receptors Data Dendritic Cells Detection Development Effector Cell Experimental Designs Flow Cytometry Fluorescent Antibody Technique Freezing Frozen Sections Gastrointestinal tract structure Gene Proteins Genes Goals Hematopoietic Hepatocyte Histology Human Image Imagery Immune Immune response Immunofluorescence Immunologic Immunohistochemistry In Vitro Individual Infection Infection Control Infiltration Inflammation Inflammatory Response Influenza Injection of therapeutic agent Injury Interferon Type I Interferon Type II Interleukin-10 KLRD1 gene Laboratories LacZ Genes Leucocytic infiltrate Leukocytes Liver Lung Lymph Node Tissue Lymphoid Tissue MHC Class I Genes Mediating Methods Microscope Microscopic Microscopy Mononuclear Mouse Strains Mus Myeloid Cells Natural Immunity Natural Killer Cells Organ Oryctolagus cuniculus Paraffin Paraffin Embedding Pathogenesis Physiology Play Population Predisposition Preparation Production Proteins Publishing Reagent Receptor Activation Regulation Reproductive Biology Research Personnel Resistance Role Route Sampling Services Site Slide Spleen Staining method Stains Structure of lymph node of thorax Surface Surface Antigens T-Lymphocyte TNFSF4 gene Technetium Tc 99m ciprofloxacin Temperature Tissue Embedding Tissue Sample Tissue Stains Tissues Training Universities Viral Viral Antigens Virginia Virus Virus Diseases Work base cell type cytokine cytomegalovirus glycoprotein gp34 experience flu in vivo influenzavirus interest lymph nodes macrophage monocyte paraform programs promoter protein expression research study resistance mechanism response spatial relationship subcutaneous tissue fixing

项目摘要

The Histology Core will provide support for histological and immunological staining of tissue samples from Projects 1-4. Appropriately prepared tissues will be embedded in paraffin or OCT for sectioning and staining. Routine histological stains will be performed. Single or 2-color immunohistochemical staining will be performed with the ABC method. For immunofluorescence, either fixed or frozen sections will be stained by Ab against lineage-specific markers for leukocyte populations, or surface costimulation molecules, adhesion molecules, receptors, and cytokines. Up to 4 color immunoflourescence staining will be performed for confocal microscopy analysis. The Histology core will also develop new reagents for studying effector cell function and development during virus infections and work closely with the Flow Cytometry Core to define Ab staining conditions and marker expression by leukocytes during virus infection. The usage of the core by the 4 projects are as follows: Project 1: Staining of mouse lung and lymph node tissues for inflammation, infiltrating cell population, and the expression of cytokine and surface receptors by CD8+ T cells, NK cells, dendritic cells, and other hematopoietic cells during influenza virus infection. Project 2: Staining of mouse liver cells for infiltrating leukocyte populations and their Ag expression and cytokine production during adenovirus infection; Reagents to block the interaction of CD8+ T cells with NK cells, dendritic cells and other cell types will be developed by the Core. Project 3: Spleen sections staining for infiltrating cells, cytokine production, and CD8+ T cell interaction with NK cells and dendritic cells during MCMV infection in susceptible and resistant mouse strains. Project 4: Staining of mouse lung and lymphoid tissues for infiltrating leukocyte subsets, surface molecules, and cytokines during influenza infection and blocking of CD8+ T cell interaction with accessory cells. The long term aim of the core is to provide support for the acquisition of immunological information on CD8+ effector cell development and function in viral infections and the influences of other cell types on CD8+ T cell functions.

组织学核心将为下列组织样本的组织学和免疫学染色提供支持项目1-4。将适当准备的组织包埋在石蜡或OCT中进行切片染色。将进行常规的组织学染色。单色或双色免疫组织化学染色将用ABC法进行。对于免疫荧光，无论是固定切片还是冰冻切片都会被染色。通过针对白细胞群体的谱系特异性标志物或表面共刺激分子的抗体，黏附分子、受体和细胞因子。将进行多达4种颜色的免疫荧光染色用于共聚焦显微镜分析。组织学核心还将开发用于研究效应器的新试剂病毒感染期间的细胞功能和发育，并与流式细胞仪核心密切合作，以明确抗体染色条件及病毒感染过程中白细胞的标志物表达。的用法这4个项目的核心内容如下：项目1：对小鼠肺和淋巴组织进行炎症、浸润性细胞群和 CD8+T细胞、NK细胞、树突状细胞等细胞因子和表面受体的表达流感病毒感染期间的造血细胞。项目2：小鼠肝细胞浸润性白细胞群染色及其抗原表达和腺病毒感染过程中细胞因子的产生；阻断CD8+T细胞与NK细胞相互作用的试剂核心将开发细胞、树突状细胞和其他类型的细胞。项目3：脾切片染色以检测浸润性细胞、细胞因子的产生和CD8+T细胞与易感和耐药小鼠MCMV感染过程中的NK细胞和树突状细胞。项目4：对小鼠肺和淋巴组织进行染色，观察白细胞亚群、表面分子、以及流感感染过程中的细胞因子和阻断CD8+T细胞与辅助细胞的相互作用。该核心的长期目标是为获取CD8+的免疫学信息提供支持病毒感染中效应细胞的发育和功能及其他细胞类型对CD8+T细胞的影响功能。