IMMUNE FUNCTIONS OF THE MACROPHAGE MAN/GLCNAC RECEPTOR

小鼠巨噬细胞Man/GlcNAc受体参与许多重要的免疫功能， 功能协调发展的 为了了解这种蛋白质的功能和生化特性， 受体，提出以下建议：（1）检查的影响， 免疫复合物对Man/GlcNAc受体功能的影响 固定化的复合物 例如DNP-抗DNP抗体或单克隆抗绵羊serythrocyte-sheep 红细胞血影将被用于调节巨噬细胞受体， 研究酵母聚糖的吞噬作用。 可溶性复合物，如TNP-牛 血清白蛋白-抗TNP抗体将用于竞争Man/GLcNAc 受体介导的辣根过氧化物酶摄取;（2）衍生 通过分离骨髓细胞建立Man/GlcNAc受体阳性细胞系 和肺泡和腹腔巨噬细胞与SV 4 O Ori- DNA;（3） Man/GlcNAc受体的鉴定和表征：（a） 免疫沉淀和聚丙烯酰胺凝胶电泳 巨噬细胞表面蛋白的抗鼠巨噬细胞异源抗血清 Man/GlcNAc受体阴性细胞系广泛吸收;（B） 通过大鼠X大鼠融合开发单克隆抗体并筛选 抗小鼠受体活性。 [125I]-抗大鼠第二抗体标记 的杂交瘤上清液处理的巨噬细胞，以及阻断 巨噬细胞对酵母聚糖和辣根过氧化物酶的摄取 将上清液用于筛选;（c）亲和标记的 通过甘露糖或辣根的标记缀合物的Man/GlcNAc受体 过氧化物酶寡糖;和（4）检查的功能作用， Man/GlcNAc受体指示巨噬细胞分泌H202 和 巨噬细胞与肿瘤细胞的结合。 寡聚核苷酸结合至 聚合物、珠或基质将用于触发巨噬细胞 分泌，而细胞糖蛋白，糖脂和寡糖 从糖缀合物中释放的糖将用于溶液中或偶联到 基质竞争肿瘤细胞与巨噬细胞的结合。 这些 实验将进一步阐明碳水化合物在免疫中的作用。 功能和Man/GlcNAc受体在巨噬细胞功能中的作用。 （MB）

The mouse macrophage Man/GlcNAc receptor serves many important immune functions. To understand the function and biochemical properties of this receptor, the following is proposed: (1) examination of the effects of immune complexes on Man/GlcNAc receptor function. Immobilized complexes such as DNP-anti DNP antibody or monoclonal anti-sheep serythrocyte-sheep erythrocyte ghosts will be used to modulate macrophage receptors of the phagocytosis of zymosan studied. Soluble complexes such as TNP-bovine serum albumin-anti-TNP antibody will be used to compete for Man/GLcNAc receptor-mediated horseradish peroxidase uptake; (2) derivation of Man/GlcNAc receptor-positive cell lines by transfecting bone marrow cells and alveolar and peritoneal macrophages with SV4O Ori- DNA; (3) identification and characterization of the Man/GlcNAc receptor by (a) immunoprecipitation and polyacrylamide gel electrophoretic analysis of macrophage surface proteins by an antimouse macrophage heteroantiserum extensively absorbed with Man/GlcNAc receptor-negative cell line; (b) development of monoclonal antibodies by rat X rat fusions and screening for anti-mouse receptor activity. [125I]-anti-rat second antibody-labeling of hybridoma supernatant-treated macrophages, as well as blockage of macrophage uptake of zymosan and horseradish peroxidase by hybridoma supernatants will be used for screening; (c) affinity-labeling of the Man/GlcNAc receptor by labeled conjugates of mannose or horseradish peroxidase oligosaccharides; and (4) examination of the functional role of the Man/GlcNAc receptor in indicating macrophage secretion of H202 and macrophage-binding to tumor cells. Oligosaccharides conjugated to polymers, beads or substrates will be used for the triggering of macrophage secretion while cellular glycoprotein, glycolipids and oligosaccharides released from the glycoconjugates will be used in solution or coupled to substrates to compete for binding of tumor cells to macrophages. These experiments will further clarify the role of carbohydrates in immune functions and the role of the Man/GlcNAc receptor in macrophage functions. (MB)