Histology and Imaging Core

组织学和成像核心

• 批准号: 7746214
• 负责人: Christopher C Norbury
• 金额: $ 22.47万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7746214
• 关键词: Animals; Area; Arts; Biohazardous Substance; Biological Response Modifiers; CD4 Positive T Lymphocytes; Cells; Chickenpox; Complex; Containment; Core Facility; Data; Dendritic Cells; Dependence; Disease; Enterovirus; Equipment; Event; Exposure to; Flavivirus; Fostering; Health; Histology; Housing; Human; Human Resources; Image; Image Analysis; Imagery; Immune; Immune system; Individual; Infection; Infectious Ectromelia; Infiltration; Institutes; Interferon Type I; Investigation; Laboratories; Life; Liver; MHC Class II Genes; Maintenance; Measles; Methods; Microscopic; Microscopy; Morbillivirus; Mouse Pox Virus; Mumps; Mus; Optics; Pathway interactions; Peripheral; Poliomyelitis; Poxviridae; Preparation; Process; Relative (related person); Research; Research Personnel; Research Project Grants; Role; Route; Rubella; Rubivirus; Rubulavirus; Sampling; Skin; Smallpox; Specimen; Spleen; Staining method; Stains; Techniques; Technology; Time; Tissue Stains; Tissues; Virion; Virus; Virus Diseases; Virus Replication; West Nile virus; Yellow Fever; cost; cryostat; design; digital imaging; experience; foot; image processing; insight; instrumentation; lymph nodes; meetings; optical imaging; pathogen; prevent; programs; research study; response; solute; trafficking

The Projects within this Center study the interplay between Ectromelia virus (ECTV), the causative agent of mousepox (the smallpox of the mouse), and its natural host, the mouse. The interplay between ECTV and the immune system of the host can take place at many different levels. It can occur at a tissue level, in which release of immune mediators such as type I interferons alter infection in the skin (Project 1) or lymph node, spleen and liver (Project 2) by ECTV., It can also occur at the cellular level, in which immune cell infiltration and interaction with ECTV infected cells will alter virus replication and spread (Project 1, 3). Finally, it can occur at an intracellular level, in which the trafficking of internalized ECTV, and the endocytic routes used during ECTV infection, can alter the mechanisms by which the immune system senses infection (Project 3 + Aim 2 of this Core). This Core (C) will allow the visualization of all of these events, by providing timely, cost-effective and expert histology and microscopy support for all three Projects within the Center. The equipment and expertise that will be provided by this Core are aimed at reducing the startup time and degree of expertise that would be necessary for individual users if they were required to design and execute experiments and image processing. In addition, the Core will allow imaging of live virus in Biosafety Level 2 containment, which is not possible in shared facilities. In addition to providing support for all three Projects, Aim 2 of this Core will investigate an area of research that bridges areas of investigation in Projects 1 and 3, namely the endocytic processes involved in ECTV internalization and infection, and the presentation of virus-derived components in complex with MHC Class II. Thus, the Core will enhance both the scientific capabilities of the Center and will foster interactions between the individual Projects.

本中心的研究项目主要是研究电虫病毒（ECTV）与致病菌之间的相互作用