Histology and Imaging Core

组织学和成像核心

• 批准号: 7746214
• 负责人: Christopher C Norbury
• 金额: $ 22.47万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7746214
• 关键词: Animals; Area; Arts; Biohazardous Substance; Biological Response Modifiers; CD4 Positive T Lymphocytes; Cells; Chickenpox; Complex; Containment; Core Facility; Data; Dendritic Cells; Dependence; Disease; Enterovirus; Equipment; Event; Exposure to; Flavivirus; Fostering; Health; Histology; Housing; Human; Human Resources; Image; Image Analysis; Imagery; Immune; Immune system; Individual; Infection; Infectious Ectromelia; Infiltration; Institutes; Interferon Type I; Investigation; Laboratories; Life; Liver; MHC Class II Genes; Maintenance; Measles; Methods; Microscopic; Microscopy; Morbillivirus; Mouse Pox Virus; Mumps; Mus; Optics; Pathway interactions; Peripheral; Poliomyelitis; Poxviridae; Preparation; Process; Relative (related person); Research; Research Personnel; Research Project Grants; Role; Route; Rubella; Rubivirus; Rubulavirus; Sampling; Skin; Smallpox; Specimen; Spleen; Staining method; Stains; Techniques; Technology; Time; Tissue Stains; Tissues; Virion; Virus; Virus Diseases; Virus Replication; West Nile virus; Yellow Fever; cost; cryostat; design; digital imaging; experience; foot; image processing; insight; instrumentation; lymph nodes; meetings; optical imaging; pathogen; prevent; programs; research study; response; solute; trafficking

The Projects within this Center study the interplay between Ectromelia virus (ECTV), the causative agent of mousepox (the smallpox of the mouse), and its natural host, the mouse. The interplay between ECTV and the immune system of the host can take place at many different levels. It can occur at a tissue level, in which release of immune mediators such as type I interferons alter infection in the skin (Project 1) or lymph node, spleen and liver (Project 2) by ECTV., It can also occur at the cellular level, in which immune cell infiltration and interaction with ECTV infected cells will alter virus replication and spread (Project 1, 3). Finally, it can occur at an intracellular level, in which the trafficking of internalized ECTV, and the endocytic routes used during ECTV infection, can alter the mechanisms by which the immune system senses infection (Project 3 + Aim 2 of this Core). This Core (C) will allow the visualization of all of these events, by providing timely, cost-effective and expert histology and microscopy support for all three Projects within the Center. The equipment and expertise that will be provided by this Core are aimed at reducing the startup time and degree of expertise that would be necessary for individual users if they were required to design and execute experiments and image processing. In addition, the Core will allow imaging of live virus in Biosafety Level 2 containment, which is not possible in shared facilities. In addition to providing support for all three Projects, Aim 2 of this Core will investigate an area of research that bridges areas of investigation in Projects 1 and 3, namely the endocytic processes involved in ECTV internalization and infection, and the presentation of virus-derived components in complex with MHC Class II. Thus, the Core will enhance both the scientific capabilities of the Center and will foster interactions between the individual Projects.

该中心的项目研究了鼠痘病毒（ECTV）与致病因子之间的相互作用。 鼠痘（小鼠的天花）及其自然宿主小鼠。ECTV之间的相互作用 并且宿主的免疫系统可以在许多不同的水平上发生。它可以发生在组织水平， 其中免疫介质如I型干扰素的释放改变皮肤中的感染（项目1），或 淋巴结、脾脏和肝脏（项目2），它也可以发生在细胞水平，其中免疫 细胞浸润和与ECTV感染细胞的相互作用将改变病毒的复制和传播（项目1，3）。 最后，它可以发生在细胞内水平，其中内化的ECTV和内吞的ECTV的运输被抑制。 在ECTV感染期间使用的途径，可以改变免疫系统感知的机制， 感染（本核心的项目3 +目标2）。该核心（C）将允许所有这些事件的可视化， 为所有三个项目提供及时，具有成本效益和专家组织学和显微镜支持， 中心该核心将提供的设备和专业知识旨在减少启动时间， 如果要求个别用户进行设计， 并进行实验和图像处理。此外，核心将允许活病毒成像， 生物安全2级防护，这在共用设施中是不可能的。除了支持 在这三个项目中，本核心的目标2将研究一个连接研究领域的研究领域 在项目1和3中，即涉及ECTV内化和感染的内吞过程，以及 病毒衍生组分与II类MHC复合物的呈递。因此，核心将增强 中心的科学能力，并将促进各个项目之间的互动。