Post Genomic Bioinformatic Core

后基因组生物信息核心

• 批准号: 7641045
• 负责人: RICHARD A SLAYDEN
• 金额: $ 16.79万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7641045
• 关键词: Animal Model; Animals; Antiviral Therapy; BCL2 gene; Bacteriophages; Bioinformatics; Biological Assay; Bioterrorism; Budgets; Burkholderia; Cells; Colorado; Congresses; Core Facility; DNA Sequence; Daily; Data; Data Analyses; Detection; Development; Event; Experimental Designs; Francisella tularensis; Future; Genome; Genomics; Goals; Health Sciences; Image Analysis; Infection; Libraries; Mass Spectrum Analysis; Microarray Analysis; Mining; Molecular; Mus; Oligonucleotides; Operative Surgical Procedures; Pathogenesis; Polymerase Chain Reaction; Preclinical Drug Evaluation; Preparation; Printing; Production; Proteins; Proteomics; Reagent; Recombinant Proteins; Recombinants; Research; Research Personnel; Research Project Grants; Resource Development; Resources; Reverse Transcriptase Polymerase Chain Reaction; Role; Sampling; Screening procedure; Services; Support of Research; Techniques; Technology; Time; Universities; Utah; Vaccines; Validation; Viral; Virus; Work; Yersinia pestis; Zoonoses; arbovirus disease; base; biodefense; comparative; data acquisition; design; detector; disorder control; experience; high throughput screening; inhibitor/antagonist; interest; mouse genome; novel; novel diagnostics; novel vaccines; pathogen; pharmacophore; programs; tandem mass spectrometry; tool

Introduction: The Genomic/Proteomics core facility will provide support to the entire Region VIII RCE. This core will function in three broad ways: (1) the core will provide specific services that are deemed universal in terms of the development and execution of post-genomic studies. To include the development and printing of genome DMA-based microarrays, array data acquisition and analysis, DMA sequencing and proteomic support such as tandem mass spectrometry and 2-D PAGE image analysis. (2) the Genomics/Proteomics Core will provide technical assistance to investigators as needed with the design of oligonucleotides for real-time-PCR and their application, proteomic analysis via 2-D-PAGE and mass spectrometry, and high throughput production of recombinant proteins and development of purification techniques and Bioinformatics. (3) Develop new postgenomic platforms for drug screening The Genomics/Proteomics Core will support the projects of the RCE in obtaining, analyzing and interpreting post-genomic information. A central theme of this RCE proposal is to develop new vaccines, diagnostics and novel chemotherapeutics against pathogens that are bioweapon agents. This core will develop post-genomic tools and resources, and accordingly provide support to each of the research projects so that these projects may exploit current and future genomic data. Project interactions: The Post Genomics Bioinformatics Core has direct connections to proposed research plans of several RCE Nodes: II.A. Bacterial Zoonoses Disease Control and Biodefense: The Post Genomics Bioinformatics Core will support this research node by providing DMA microarrays, and performing proteomic-related mass spectrometry. The core will also assist in the validation of post-genomic data via RT-PCR and the production of recombinant products. In addition Aim 3. of the core will be integral with the development of novel inhibitors by providing HT screening on compound libraries (such as M.A.2., II.A.4.) II.B. Arboviral Disease Control and Biodefense: The major contribution to this project by the Post Genomics Bioinformatics Core is envisioned to be primarily in providing support for use of the mouse DMA microarrays, immunoregulatory DMA microarray and RT-PCR technologies. The Viral Zoonoses Program will also benefit from the production of recombinant products and mass spectrometry support (section D.1.f.). II.C. UCHSC Molecular Pathogenesis of Burkholderia Select Agents: The University of Colorado Health Sciences Project encompassing Projects II.C.1.-II.C.6. will be supported by the Post Genomics Bioinformatics Core in a similar fashion to the other projects. The core will support post-genomic studies involving DMA microarrays, molecular identification of proteins by mass spectrometry and high throughput screening. II.D. Development of Bacteriophage Amplification Reagents and Immuno-detectors for Y. pestis and F. tularensis (CSM). The Post Genomics Bioinformatics Core will provide bioinformatics support for comparative genomics and genome mining. II.G. Antiviral therapies for potential Bioterrorism Viruses (Utah State University Research Project): The Utah State University Research Project (Project II.G.2.) will obtain assistance making a VEE construct (TC-83 vaccine strain) expressing Bcl-2 gene. Other support could include whole mouse array and aspects of recombinant protein production. The cores in this RCE proposal have an integral relationship to each other. The Post Genomics Bioinformatics Core will support the Animal Core (I.B.S.a) by providing tools and reagents to help characterize the animal models of infection on the genomic scale. This support will primarily come in the form of the whole genome mouse array, the immunoregulatory direct array and the real time PCR technologies for sequence detection. In addition, work done in the Product Development and Manufacturing Core (I.B.S.b.) will benefit from the Post Genomics Bioinformatics Core facility because of the recombinant products support and mass spectrometry support.

简介：基因组学/蛋白质组学核心设施将为整个区域VIII RCE提供支持。这一核心 将以三种广泛的方式发挥作用：（1）核心将提供被认为是普遍的具体服务， 后基因组研究的开发和执行。包括基因组的开发和打印 基于DMA的微阵列、阵列数据采集和分析、DMA测序和蛋白质组学支持，如 串联质谱和2-D PAGE图像分析。(2)基因组学/蛋白质组学核心将提供 根据需要为研究人员提供技术援助，设计用于实时PCR的寡核苷酸， 应用，通过2-D-PAGE和质谱进行蛋白质组学分析，以及高通量生产 重组蛋白质和纯化技术和生物信息学的发展。(3)开发新的后基因组 基因组学/蛋白质组学核心将支持RCE的项目， 获取、分析和解释后基因组信息。该RCE提案的一个中心主题是 开发新的疫苗、诊断方法和新的化学疗法，以对付作为生物武器剂的病原体。 该核心将开发后基因组工具和资源，并相应地为每个研究提供支持 这些项目可以利用当前和未来的基因组数据。项目互动：The Post 基因组学生物信息学核心与几个RCE节点的拟议研究计划有直接联系： 细菌性人畜共患病疾病控制和生物防御：后基因组学生物信息学核心将支持 通过提供DMA微阵列和进行蛋白质组学相关的质谱分析，这一研究节点。的 核心还将协助通过RT-PCR验证后基因组数据和生产重组 产品.此外，目标3。核心将通过提供HT与新型抑制剂的开发不可分割 在化合物文库上筛选（如M.A.2.，（第二.A.4段）二.b.虫媒病毒疾病控制和生物防御： 后基因组学生物信息学核心对该项目的主要贡献预计主要在 为使用小鼠DNA微阵列、免疫调节DNA微阵列和RT-PCR提供支持 技术.病毒性人畜共患病方案也将受益于重组产品的生产， 质谱支持（第D.1.f.节）。二.c.伯克霍尔德氏菌的UCHSC分子致病机制 代理人：科罗拉多大学健康科学项目，包括项目II.C.1.-二.C.6.将 由后基因组学生物信息学核心以类似于其他项目的方式支持。芯会 支持后基因组研究，包括DMA微阵列，通过质量进行蛋白质的分子鉴定 光谱法和高通量筛选。二、D.噬菌体扩增试剂的研究进展 免疫检测仪检测Y. pestis和F.土拉热菌（CSM）。后基因组学生物信息学核心将 为比较基因组学和基因组挖掘提供生物信息学支持。二. G.抗病毒治疗 潜在的生物恐怖主义病毒（犹他州州立大学研究项目）：犹他州州立大学研究 项目（项目二.G.2.）将获得援助，以制造表达Bcl-2的VEE构建体（TC-83疫苗株 基因其他支持可能包括整个小鼠阵列和重组蛋白生产方面。核 在该RCE建议中，它们彼此之间具有不可分割关系。后基因组学生物信息学核心将 通过提供工具和试剂来支持动物核心（I.B.S.a），以帮助表征以下动物模型： 基因组规模的感染。这种支持将主要以全基因组小鼠阵列的形式出现， 免疫调节直接芯片和真实的时间PCR技术进行序列检测。此外，工作 在产品开发和制造中心（I.B.S.b.）完成将受益于后基因组学 生物信息学核心设施，因为重组产品的支持和质谱分析的支持。