Coxiella Cultivation Core

柯克斯体培养核心

• 批准号: 7641042
• 负责人: Michael F Minnick
• 金额: $ 22.76万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7641042
• 关键词: Acute; Aerosols; Alveolar Macrophages; Attenuated; Bacteria; Basic Science; Candidate Disease Gene; Cells; Characteristics; Clinical; Cloning; Collaborations; Complement; Containment; Core Facility; Coupled; Coxiella; Coxiella burnetii; Cytolysis; Data; Development; Dust; Endocarditis; Environment; Gene Expression; General Practitioners; Generations; Genes; Goals; Head; Hepatitis; Human; Human Resources; In Vitro; Individual; Infection; Insect Bites; Invasive; Knock-out; Life Cycle Stages; Location; Mammals; Milk; Mind; Modeling; Molecular; Molecular Profiling; Montana; Morphogenesis; Mus; Mutagenesis; Natural History; Nature; Open Reading Frames; Organism; Parasites; Pathogenesis; Phase; Pneumonia; Polyvalent Vaccine; Population; Proteins; Purpose; Range; Relative (related person); Research; Role; Route; Services; Shuttle Vectors; Site-Directed Mutagenesis; Spontaneous abortion; Staging; System; Testing; Ticks; Time; Training; Universities; Vaccine Design; Vaccine Production; Variant; Virulence; Virulence Factors; Work; base; biosafety level 3 facility; cell type; design; experience; extracellular; genetic manipulation; in vivo; infancy; mutant; pathogen; programs; research study; trait; transmission process

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly. These characteristics, coupled with C. burnetifs highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii"\s essential to the proposed research, a dedicated core facility with BSL-3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core is to make available to each of the 4 projects expertise in the cultivation, enumeration, and archival of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, but will be headed by Mike Minnick of the University of Montana. Collaborations among projects. Core A will provide services to each of the 4 projects of the Program Project. These services will include making standardized inocula of phase 1 and 2 Coxiella available to all the cores, as well of the services of Coxiella enumeration and archival of Coxiella organisms. In addition, projects 2, 3 and 4 have no experience with working with Coxiella, thus, the Core will provide this additional critical service to those projects. Therefore, the core is integral and critical to the goals of each of the projects and the goal of the Program Project.

专性细胞内病原体的繁殖和定量都是劳动密集型和昂贵的。这些 特性，再加上C. burnetifs高度传染性和选择代理指定，复杂 最基本的研究工作。然而，由于C.伯内特氏 对拟议的研究至关重要的是，一个专用的核心设施，具有BSL-3安全壳，训练有素的技术 人员，并提出了一个档案系统。因此，核心的目的是使每个人都能获得 在4个项目中，拥有1期和2期贝氏柯克斯体培养、计数和存档方面的专业知识。 核心将位于蒙大拿州立大学，BSL-3设施的位置，但将领导 蒙大拿大学的迈克·明尼克。 项目之间的合作。核心A将为该计划的4个项目提供服务 项目这些服务将包括向所有人提供1期和2期柯克斯体的标准化接种 的核心，以及服务的柯克斯体计数和档案柯克斯体生物。此外，本发明还提供了一种方法， 项目2、3和4没有与Coxiella合作的经验，因此，核心将提供额外的 这些项目的关键服务。因此，核心是不可或缺的，对每个国家的目标至关重要。 项目和计划的目标。