Coxiella Cultivation Core

柯克斯体培养核心

基本信息

批准号：
7641042
负责人：
Michael F Minnick
金额：
$ 22.76万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7641042
关键词：
Acute Aerosols Alveolar Macrophages Attenuated Bacteria Basic Science Candidate Disease Gene Cells Characteristics Clinical Cloning Collaborations Complement Containment Core Facility Coupled Coxiella Coxiella burnetii Cytolysis Data Development Dust Endocarditis Environment Gene Expression General Practitioners Generations Genes Goals Head Hepatitis Human Human Resources In Vitro Individual Infection Insect Bites Invasive Knock-out Life Cycle Stages Location Mammals Milk Mind Modeling Molecular Molecular Profiling Montana Morphogenesis Mus Mutagenesis Natural History Nature Open Reading Frames Organism Parasites Pathogenesis Phase Pneumonia Polyvalent Vaccine Population Proteins Purpose Range Relative (related person)Research Role Route Services Shuttle Vectors Site-Directed Mutagenesis Spontaneous abortion Staging System Testing Ticks Time Training Universities Vaccine Design Vaccine Production Variant Virulence Virulence Factors Work base biosafety level 3 facility cell type design experience extracellular genetic manipulation in vivo infancy mutant pathogen programs research study trait transmission process

项目摘要

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly. These characteristics, coupled with C. burnetifs highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii"\s essential to the proposed research, a dedicated core facility with BSL-3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core is to make available to each of the 4 projects expertise in the cultivation, enumeration, and archival of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, but will be headed by Mike Minnick of the University of Montana. Collaborations among projects. Core A will provide services to each of the 4 projects of the Program Project. These services will include making standardized inocula of phase 1 and 2 Coxiella available to all the cores, as well of the services of Coxiella enumeration and archival of Coxiella organisms. In addition, projects 2, 3 and 4 have no experience with working with Coxiella, thus, the Core will provide this additional critical service to those projects. Therefore, the core is integral and critical to the goals of each of the projects and the goal of the Program Project.

义务细胞内病原体的传播和量化既是劳动密集型且昂贵的。这些特征，加上C. burnetifs高度传染性和精选药物名称，使其复杂最基本的研究努力。但是，由于高度纯化和枚举的C. burnetii“ \ s 对于拟议研究的必不可少提出了人员和档案系统。因此，核心的目的是向每个人提供在第1和2阶段Coxiella burnetii的耕种，枚举和档案中的4个项目专业知识中。核心将位于蒙大拿州立大学（BSL-3设施的位置），但将领导蒙大拿大学的迈克·米尼克（Mike Minnick）。项目之间的合作。核心A将为程序的四个项目提供服务项目。这些服务将包括使所有人都可以使用标准化的1和2 Coxiella的接种物核心以及柯西拉有机体的库克西拉枚举和档案的核心。此外，项目2、3和4没有与Coxiella合作的经验，因此，核心将提供此额外这些项目的关键服务。因此，核心对每个目标的目标都是不可或缺的项目和计划项目的目标。