Coxiella Cultivation Core

专性细胞内病原体的繁殖和量化既是劳动密集型的，也是昂贵的。这些 伯氏梭菌的特性，加上高度传染性的性质和选定的药剂指定，使情况变得复杂 最基础的研究努力。然而，由于高度纯化和计数的伯氏梭菌“\S” 对拟议的研究至关重要，一个配备BSL-3安全壳的专用核心设施，训练有素的技术人员 人员，并提出了档案系统。因此，核心的目的是使每个人都可以使用 在4个项目中，有1期和2期贝氏柯克斯体的培养、计数和存档方面的专门知识。 核心将位于BSL-3设施所在地蒙大拿州立大学，但将负责 作者是蒙大拿大学的迈克·明尼克。 项目之间的协作。核心A将为该计划的4个项目中的每个项目提供服务 项目。这些服务将包括向所有人提供标准化的1期和2期柯克斯体疫苗接种 核心，以及柯克斯体计数和柯克斯体档案服务。此外, 项目2、3和4没有与Coxiella合作的经验，因此，核心将提供这一额外的 为这些项目提供关键服务。因此，核心是不可或缺的，对每一个 项目和计划项目的目标。

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly. These characteristics, coupled with C. burnetifs highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii"\s essential to the proposed research, a dedicated core facility with BSL-3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core is to make available to each of the 4 projects expertise in the cultivation, enumeration, and archival of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, but will be headed by Mike Minnick of the University of Montana. Collaborations among projects. Core A will provide services to each of the 4 projects of the Program Project. These services will include making standardized inocula of phase 1 and 2 Coxiella available to all the cores, as well of the services of Coxiella enumeration and archival of Coxiella organisms. In addition, projects 2, 3 and 4 have no experience with working with Coxiella, thus, the Core will provide this additional critical service to those projects. Therefore, the core is integral and critical to the goals of each of the projects and the goal of the Program Project.