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Coxiella Cultivation Core

柯克斯体培养核心

基本信息

批准号：
7641042
负责人：
Michael F Minnick
金额：
$ 22.76万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7641042
关键词：
Acute Aerosols Alveolar Macrophages Attenuated Bacteria Basic Science Candidate Disease Gene Cells Characteristics Clinical Cloning Collaborations Complement Containment Core Facility Coupled Coxiella Coxiella burnetii Cytolysis Data Development Dust Endocarditis Environment Gene Expression General Practitioners Generations Genes Goals Head Hepatitis Human Human Resources In Vitro Individual Infection Insect Bites Invasive Knock-out Life Cycle Stages Location Mammals Milk Mind Modeling Molecular Molecular Profiling Montana Morphogenesis Mus Mutagenesis Natural History Nature Open Reading Frames Organism Parasites Pathogenesis Phase Pneumonia Polyvalent Vaccine Population Proteins Purpose Range Relative (related person)Research Role Route Services Shuttle Vectors Site-Directed Mutagenesis Spontaneous abortion Staging System Testing Ticks Time Training Universities Vaccine Design Vaccine Production Variant Virulence Virulence Factors Work base biosafety level 3 facility cell type design experience extracellular genetic manipulation in vivo infancy mutant pathogen programs research study trait transmission process

项目摘要

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly. These characteristics, coupled with C. burnetifs highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii"\s essential to the proposed research, a dedicated core facility with BSL-3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core is to make available to each of the 4 projects expertise in the cultivation, enumeration, and archival of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, but will be headed by Mike Minnick of the University of Montana. Collaborations among projects. Core A will provide services to each of the 4 projects of the Program Project. These services will include making standardized inocula of phase 1 and 2 Coxiella available to all the cores, as well of the services of Coxiella enumeration and archival of Coxiella organisms. In addition, projects 2, 3 and 4 have no experience with working with Coxiella, thus, the Core will provide this additional critical service to those projects. Therefore, the core is integral and critical to the goals of each of the projects and the goal of the Program Project.

专性细胞内病原体的繁殖和定量是劳动密集型和昂贵的。这些