Protein Expression Core

蛋白质表达核心

• 批准号: 7706297
• 负责人: MARLENE BELFORT
• 金额: $ 30.11万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7706297
• 关键词: Animals; Antibodies; Antigens; Area; Biological Assay; Development; Diagnostic; Emergency Situation; Goals; Hand; Investigation; Molecular Genetics; Pilot Projects; Production; Protein Biochemistry; Protein Fragment; Proteins; Quality Control; Recombinant Proteins; Research Personnel; Scientist; Services; Standards of Weights and Measures; System; Therapeutic; Time; Translations; Vaccines; Work; design; expression vector; novel vaccines; protein expression; vaccine development; vector

The Protein Expression Core will draw on the significant strengths of the Wadsworth Center in the areas of molecular genetics and protein biochemistry. The goal of the Protein Production Core will be to facilitate the investigative, developmental, and pilot studies proposed in various themes of this application. The staff of the Core will assist investigators in the translation of their studies to quality controlled standard assays, vaccines or therapeutics. Staff of the Protein Expression Core will work with investigators to develop expression systems for the antigens required for their studies either using commercial vectors or customized expression vectors. The facility will provide a complete protein expression and purification service. The staff of the Protein Production Core will produce highly purified standardized lots of recombinant protein antigens, recombinant proteins or protein fragments, purified antibodies and modified proteins as needed by the investigators for the development of multiplex diagnostic assays and novel vaccines. In the case of vaccine development, the Core will provide proteins with low bacterial component contamination suitable for use in animal studies. The Core is designed to provide both advisory and hands on support to scientists, who need larger amounts of purified recombinant proteins for their investigations. A goal of the Core will be to prepare stable stocks of proteins and expression strains so that in a time of emergency production of these materials will be rapid.

蛋白质表达核心将利用沃兹沃思中心在 分子遗传学和蛋白质生物化学。蛋白质生产核心的目标是促进 在本应用的各个主题中提出的调查，发育和试点研究。工作人员 核心将协助研究人员将研究转化为质量控制的标准测定法， 疫苗或治疗剂。蛋白质表达核心的工作人员将与研究人员合作开发 使用商业向量或定制的研究所需的抗原的表达系统 表达向量。该设施将提供完整的蛋白质表达和纯化服务。工作人员 蛋白质生产的核心将产生高度纯化的标准化大量重组蛋白抗原， 重组蛋白或蛋白质片段，纯化的抗体和修饰的蛋白 研究人员开发多重诊断测定和新型疫苗。如果是疫苗 开发，核心将为蛋白质提供低细菌成分污染的污染物 动物研究。该核心旨在为需要的科学家提供咨询和支持 大量纯化的重组蛋白进行研究。核心的目标是准备 蛋白质和表达菌株的稳定库存，因此在紧急生产这些材料的时候 会很快。