Imaging and reversible inactivation of indirect inputs to area MT

区域 MT 间接输入的成像和可逆失活

• 批准号: 7622634
• 负责人: Jonathan J Nassi
• 金额: $ 5.01万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7622634
• 关键词: Affect; Behavior; Behavioral; Brain; Brain region; Cells; Chills; Complex; Disease; Eye Movements; Generations; Goals; Image; Implant; Label; Lead; Link; Lunate Sulcus; Macaca; Measures; Mediating; Mental disorders; Methanol; Microscopy; Monkeys; Motion; Motion Perception; Parkinson Disease; Pathway interactions; Perception; Performance; Primates; Process; Property; Research; Role; Schizophrenia; Source; Symptoms; Vision Disparity; Visual; Visual Cortex; Visual Motion; Visual system structure; area MT; awake; insight; interest; mental age; neural circuit; neuromechanism; response; two-photon

DESCRIPTION (provided by applicant): The primary goal is to investigate the functional organization of motion computations in visual cortex. Primate area MT is intimately linked to the perception of motion and the generation of eye movements, yet little is known regarding the functional role of each of its multiple sources of convergent input. This information is critical to our understanding of how neural circuits underlie perception and behavior, and may have important implications for disorders of the visual system. The proposed studies will focus on primate area MT and will assess the specific contributions of inputs from V2 using reversible inactivation and two photon microscopy. We will first retrogradely label cells that project from V2 to MT and image their activity using two-photon microscopy. The visual response properties of MT-projecting cells will be characterized and compared to other cells nearby and across functional compartments within V2. Of particular interest will be response properties thought to be computed first within MT as well as those thought to be relayed to MT through V2 rather than other known input pathways. Next, we will reversibly inactivate V2 with chilled methanol perfused through coils implanted into the lunate sulcus. MT response properties and behavioral performance can then be measured in the awake, behaving macaque monkey with and without an active V2. Special emphasis will be given to investigating the role of V2 in providing MT with direction of motion and binocular disparity information. This research will lead to a better understanding of how our brain processes a visual scene and mediates our ability to perceive moving objects. Not only will this information elucidate the complex processes of the healthy brain, but it will also give us important insight into the brain regions affected by aging and mental disease. In particular, several mental disorders such as schizophrenia and Parkinson's disease have selective deficits in visual motion perception, and a better understanding of the neural mechanisms involved in these conditions may inform efforts to alleviate these symptoms

描述（由申请人提供）：主要目标是研究视觉皮层运动计算的功能组织。灵长类区域 MT 与运动感知和眼球运动的产生密切相关，但对其多个汇聚输入源的功能作用知之甚少。这些信息对于我们理解神经回路如何构成感知和行为至关重要，并且可能对视觉系统疾病产生重要影响。拟议的研究将重点关注灵长类动物区域 MT，并将使用可逆失活和双光子显微镜评估 V2 输入的具体贡献。我们将首先逆行标记从 V2 投射到 MT 的细胞，并使用双光子显微镜对其活动进行成像。 MT 投射细胞的视觉反应特性将被表征，并与 V2 内功能区室附近的其他细胞进行比较。特别令人感兴趣的是被认为首先在 MT 内计算的响应属性以及被认为通过 V2 而不是其他已知输入路径中继到 MT 的响应属性。接下来，我们将通过植入月骨沟的线圈灌注冷冻甲醇来可逆地灭活 V2。然后可以在清醒、有行为的猕猴（有或没有活跃 V2）中测量 MT 反应特性和行为表现。将特别强调研究 V2 在为 MT 提供运动方向和双眼视差信息方面的作用。这项研究将有助于更好地理解我们的大脑如何处理视觉场景并调节我们感知移动物体的能力。这些信息不仅可以阐明健康大脑的复杂过程，而且还可以让我们深入了解受衰老和精神疾病影响的大脑区域。特别是，精神分裂症和帕金森病等几种精神疾病在视觉运动感知方面存在选择性缺陷，更好地了解这些疾病所涉及的神经机制可能有助于缓解这些症状