Mouse Core

非侵入性映射特定分子，细胞和病理生理信息的能力， 小鼠模型和异种移植将是该计划成功的关键。具体来说，成像在 活体动物在检测癌症发生和进展期间的早期异常方面是必不可少的， 建立成像-表型相关性，验证靶点和相关生物标志物， 治疗剂的治疗功效。核心服务于两个特定目的：a）作为高科技核心， 智力资源，以执行和协助成像实验和B）作为创新研究核心 以进一步开发不同项目所需的新成像方法。 核心提供的成像技术范围从微观到宏观分辨率 技术，所有高度优化的鼠标成像。具体的成像技术包括活体共焦 显微镜，荧光反射成像，光纤荧光成像，荧光介导 断层扫描（FMT）、生物发光成像、单光子计算机发射断层扫描（SPECT）， 正电子发射断层扫描（PET）、X射线计算机断层扫描（CT）和磁共振成像。一 最近开发的基于网络的生物信息学平台（CMIR-MIPortal）将确保获得成像 研究，并使基因组/蛋白质组数据与表型或分子 成像数据。该系统是我们基于网络的通信系统的一个组成部分， 50常成员之间的信息交流。核心也将作为一个中央管道， 合作者（DePinho和Jacks博士）获得独特的小鼠模型，并在合作中验证 新型显像剂的应用。最后，核心将协助所有实验病理学研究，以确保 最高程度的专业知识。

The ability to non-invasively map specific molecular, cellular and pathophysiologic information in mouse models and in xenotransplants will be critical to the success of the program. Specifically, imaging in live animals will be essential in detecting early abnormalities during cancer initiation and progression, in establishing imaging-phenotype correlations, in validating targets and linked biomarkers, and in assessing therapeutic efficacy of therapeutic agents. The core serves two specific purposes: a) as a high-tech core and intellectual resource to perform and assist with imaging experiments and b) as an innovative research core to further develop novel imaging approaches required in the different projects. The imaging technologies provided by the core range from microscopic to macroscopic resolution techniques, all highly optimized for mouse imaging. Specific imaging techniques include intravital confocal microscopy, fluorescence reflectance imaging, fiberoptic fluorescence imaging, fluorescence-mediated tomography (FMT), bioluminescence imaging, single photon computed emission tomography (SPECT), positron emission tomography (PET), x-ray computed tomography (CT) and magnetic resonance imaging. A recently developed web-based bioinformatics platform (CMIR-MIPortal¿) will assure access to imaging studies and enable refined combination and query of genomic/proteomic data with phenotypic or molecular imaging data. This system is one component of our web-based communication system that permits regular exchange of information among the P50 membership. The core will also act as a central conduit to our collaborators (Drs. dePinho and Jacks) to gain access to unique mouse models and in collaboration, validate the use of novel imaging agent. Finally, the Core will assist with all experimental pathology studies to assure the highest degree of expertise.