High-throughput Screening Core

高通量筛选（HTS）核心实验室（Core B）的目的是创建和进行HTS 筛选小分子，以确定那些可以成为开发癌症治疗药物的先导物， 癌症研究中的工具。此外，HTS实验室进行基因组RNAi筛选，以确定新的 用于开发癌症疗法的靶点。 核心B管理UTSW化学化合物文件，目前包括20多万种小类药物 有机化合物核心B为PPG的项目开发分析，筛选并分析 筛选数据。HTS实验室进行验证感兴趣化合物所需的二次筛选 在初步筛选实验中发现。与PPG的研究人员合作， 核心B和化学核心优先考虑HTS实验中鉴定的感兴趣的化合物， 管理这些化合物的补给和储存。HTS核心维护Oracle数据库， 筛选数据，以便在任何HTS实验中感兴趣的化合物的活性可以与所有HTS实验进行比较。 用UTSW复合文件进行的其他HTS实验。HTS核心还管理和 为PPG的项目进行人类基因组siRNA文库的HTS筛选实验。 除了对NCI资助的PPG成员进行HTS筛选外，核心B还提供培训和建议 UT西南综合癌症中心的成员对开发和执行 小分子和基因组siRNA HTS实验，并将来自这些实验的数据存储为 UTSW筛查数据库 HTS设施通过为癌症研究人员提供数百种 数千种结构不同的化合物，可用于癌症的药物治疗或工具 研究可以识别。

The purpose of the high-throughput screening (HTS) core laboratory (Core B) is to create and conduct HTS screens of small molecules to identify those that can become leads for developing cancer therapeutics or tools in cancer research. In addition, the HTS laboratory conducts genomic RNAi screens to identify novel targets for developing cancer therapeutics. Core B manages the UTSW chemical compound file currently including more than 200,000 small drug-like organic compounds. Core B develops assays for the projects of the PPG,screens them and analyzes screening data. The HTS lab conducts secondary screens required to validate compounds of interest identified during primary screening experiments. Working with the investigators of the PPG, scientists in Core B and the Chemistry Core prioritize the compounds of interest identified in HTS experiments and manage resupply and storage of those compounds. The HTS core maintains an Oracle database of screening data so that the activity of compounds of interest in any HTS experiment can be compared to all other HTS experiments conducted with the UTSW compound file. The HTS Core also manages and conducts HTS screening experiments of a human genomic siRNA library for the Projects of the PPG. In addition to conducting HTS screens for members of this NCI funded PPG, Core B trains and advises members of the Comprehensive Cancer Center at UT Southwestern on the development and execution of small molecule and genomic siRNA HTS experiments and stores the data from such experiments as part of the UTSW screening database. The HTS facility advances cancer research by providing cancer researchers access to hundreds of thousands of structurally diverse compounds from which leads for drug therapeutics or tools for cancer research can be identified.